Clock (NM_001289826) Mouse Tagged ORF Clone
CAT#: MR231264
- TrueORF®
Clock (myc-DDK-tagged) - Mouse circadian locomotor output cycles kaput (Clock), transcript variant 2
"NM_001289826" in other vectors (1)
Need custom modification / cloning service?
Get a free quote
CNY 6,264.00
CNY 7,130.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 5330400M04Rik; KAT13D |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231264 representing NM_001289826
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTTTACCGTAAGCTGTAGTAAAATGAGCTCAATTGTTGACAGAGATGACAGTAGTATTTTTGATG GATTGGTGGAAGAAGATGACAAGGACAAAGCAAAAAGAGTATCTAGAAACAAATCAGAAAAGAAACGTAG AGATCAGTTCAATGTCCTCATTAAGGAGCTGGGGTCTATGCTTCCTGGTAACGCGAGAAAGATGGACAAG TCTACTGTTCTACAGAAGAGCATTGATTTTTTGCGCAAACATAAAGAGACCACTGCACAGTCAGATGCTA GTGAGATTCGACAGGACTGGAAACCCACATTCCTTAGTAATGAAGAGTTTACACAGTTAATGTTAGAGGC TCTTGATGGTTTTTTTTTAGCGATCATGACAGATGGAAGTATAATATATGTATCTGAGAGTGTAACTTCG TTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTTATCCCAGAGGGAGAACATT CAGAGGTTTATAAGATACTCTCTACTCATCTGCTGGAAAGTGACTCATTAACCCCTGAGTACTTAAAATC AAAAAATCAGTTAGAATTCTGTTGTCACATGCTTCGAGGAACAATAGACCCAAAGGAGCCATCCACCTAT GAATATGTGAGATTTATAGGAAATTTTAAATCTTTAACCAGTGTATCAACTTCAACACACAATGGTTTTG AAGGAACTATACAACGCACACATAGGCCTTCTTATGAAGATAGAGTTTGTTTTGTAGCTACTGTCAGATT AGCTACACCTCAGTTCATCAAGGAAATGTGTACTGTTGAAGAACCAAATGAAGAGTTTACATCTAGACAC AGTTTAGAATGGAAGTTTCTATTTTTAGATCACAGGGCACCACCAATAATAGGCTATTTGCCATTTGAAG TCTTGGGAACATCAGGCTATGATTACTATCATGTGGATGACCTAGAAAATCTGGCAAAATGTCACGAGCA CTTAATGCAATATGGAAAAGGCAAATCGTGTTACTATAGATTCCTGACCAAAGGCCAGCAGTGGATATGG CTTCAGACTCATTATTATATTACTTACCATCAGTGGAATTCAAGGCCAGAGTTCATTGTTTGTACTCACA CTGTAGTAAGTTATGCAGAAGTTAGGGCTGAAAGACGGCGAGAACTTGGCATTGAAGAGTCTCTTCCTGA GACAGCTGCTGACAAAAGCCAAGATTCTGGGTCTGACAATCGTATCAACACAGTGAGTCTCAAGGAAGCA CTGGAAAGGTTTGATCACAGCCCAACTCCTTCTGCCTCCTCTAGAAGCTCACGAAAGTCATCTCACACCG CAGTCTCAGACCCTTCCTCCACACCGACAAAGATCCCTACTGATACTAGCACTCCTCCCAGACAGCATTT GCCAGCTCATGAAAAGATGACACAGCGGAGGTCGTCCTTCAGCAGTCAGTCCATAAACTCCCAGTCAGTT GGTCCATCATTAACACAGCCAGCGATGTCTCAAGCTGCAAATTTACCAATTCCACAAGGCATGTCACAGT TTCAGTTTTCAGCTCAGTTAGGAGCCATGCAGCATCTAAAAGACCAGCTAGAGCAGCGGACACGGATGAT AGAGGCAAATATTCATCGGCAGCAAGAAGAACTAAGGAAAATTCAAGAGCAACTTCAGATGGTCCATGGT CAAGGGCTACAGATGTTTTTGCAGCAATCAAACCCTGGATTGAATTTTGGTTCTGTTCAACTTTCCTCTG GAAATTCTAATATCCAGCAACTCACACCTGTAAATATGCAAGGCCAGGTTGTCCCTGCTAACCAGGTTCA GAGTGGACATATCAGCACAGGCCAGCACATGATACAGCAACAGACTTTACAAAGTACATCAACTCAGCAG AGTCAACAGAGTGTAATGAGTGGACACAGTCAGCAGACGTCTCTTCCAAGTCAGACACCGAGCACTCTCA CAGCCCCACTGTACAATACGATGGTGATTTCCCAGCCTGCAGCTGGGAGCATGGTCCAGATTCCATCCAG TATGCCACAGAACAGTACCCAGAGTGCTACAGTCACTACGTTCACTCAGGACAGACAGATAAGATTTTCT CAAGGTCAGCAACTTGTGACCAAATTAGTGACTGCTCCTGTAGCTTGTGGGGCCGTCATGGTACCAAGTA CCATGCTTATGGGTCAGGTGGTGACTGCCTATCCTACCTTCGCCACACAACAGCAGCAGGCACAGACATT ATCGGTAACACAACAGCAGCAGCAGCAGCAGCAGCAGCCACCACAGCAACAGCAACAACAACAGCAGAGT TCCCAGGAACAGCAGCTTCCTTCAGTTCAGCAGCCAGCTCAGGCCCAGCTGGGCCAGCCACCACAGCAGT TCTTACAGACATCTAGGTTGCTCCACGGGAATCCTTCGACACAGCTCATCCTCTCTGCTGCCTTTCCACT ACAACAGAGCACTTTCCCTCCTTCGCACCACCAGCAACACCAGCCTCAGCAGCAACAGCAGCTTCCTCGG CACAGGACTGACAGCCTGACTGACCCTTCCAAGGTCCAGCCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231264 representing NM_001289826
Red=Cloning site Green=Tags(s) MVFTVSCSKMSSIVDRDDSSIFDGLVEEDDKDKAKRVSRNKSEKKRRDQFNVLIKELGSMLPGNARKMDK STVLQKSIDFLRKHKETTAQSDASEIRQDWKPTFLSNEEFTQLMLEALDGFFLAIMTDGSIIYVSESVTS LLEHLPSDLVDQSIFNFIPEGEHSEVYKILSTHLLESDSLTPEYLKSKNQLEFCCHMLRGTIDPKEPSTY EYVRFIGNFKSLTSVSTSTHNGFEGTIQRTHRPSYEDRVCFVATVRLATPQFIKEMCTVEEPNEEFTSRH SLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHVDDLENLAKCHEHLMQYGKGKSCYYRFLTKGQQWIW LQTHYYITYHQWNSRPEFIVCTHTVVSYAEVRAERRRELGIEESLPETAADKSQDSGSDNRINTVSLKEA LERFDHSPTPSASSRSSRKSSHTAVSDPSSTPTKIPTDTSTPPRQHLPAHEKMTQRRSSFSSQSINSQSV GPSLTQPAMSQAANLPIPQGMSQFQFSAQLGAMQHLKDQLEQRTRMIEANIHRQQEELRKIQEQLQMVHG QGLQMFLQQSNPGLNFGSVQLSSGNSNIQQLTPVNMQGQVVPANQVQSGHISTGQHMIQQQTLQSTSTQQ SQQSVMSGHSQQTSLPSQTPSTLTAPLYNTMVISQPAAGSMVQIPSSMPQNSTQSATVTTFTQDRQIRFS QGQQLVTKLVTAPVACGAVMVPSTMLMGQVVTAYPTFATQQQQAQTLSVTQQQQQQQQQPPQQQQQQQQS SQEQQLPSVQQPAQAQLGQPPQQFLQTSRLLHGNPSTQLILSAAFPLQQSTFPPSHHQQHQPQQQQQLPR HRTDSLTDPSKVQPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001289826 |
ORF Size | 2565 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001289826.1, NP_001276755.1 |
RefSeq Size | 9758 bp |
RefSeq ORF | 2568 bp |
Locus ID | 12753 |
UniProt ID | O08785 |
MW | 96.4 kDa |
Gene Summary | The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with Arntl (Bmal1) that binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Polymorphisms in this gene may be associated with behavioral changes, obesity, and metabolic syndrome. Two transcripts encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC229053 | Clock (untagged) - Mouse circadian locomotor output cycles kaput (Clock), transcript variant 2 |
CNY 6,272.00 |