Clock (NM_001289826) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5330400M04Rik; KAT13D
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229053 representing NM_001289826 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTTTACCGTAAGCTGTAGTAAAATGAGCTCAATTGTTGACAGAGATGACAGTAGTATTTTTGATG GATTGGTGGAAGAAGATGACAAGGACAAAGCAAAAAGAGTATCTAGAAACAAATCAGAAAAGAAACGTAG AGATCAGTTCAATGTCCTCATTAAGGAGCTGGGGTCTATGCTTCCTGGTAACGCGAGAAAGATGGACAAG TCTACTGTTCTACAGAAGAGCATTGATTTTTTGCGCAAACATAAAGAGACCACTGCACAGTCAGATGCTA GTGAGATTCGACAGGACTGGAAACCCACATTCCTTAGTAATGAAGAGTTTACACAGTTAATGTTAGAGGC TCTTGATGGTTTTTTTTTAGCGATCATGACAGATGGAAGTATAATATATGTATCTGAGAGTGTAACTTCG TTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTTATCCCAGAGGGAGAACATT CAGAGGTTTATAAGATACTCTCTACTCATCTGCTGGAAAGTGACTCATTAACCCCTGAGTACTTAAAATC AAAAAATCAGTTAGAATTCTGTTGTCACATGCTTCGAGGAACAATAGACCCAAAGGAGCCATCCACCTAT GAATATGTGAGATTTATAGGAAATTTTAAATCTTTAACCAGTGTATCAACTTCAACACACAATGGTTTTG AAGGAACTATACAACGCACACATAGGCCTTCTTATGAAGATAGAGTTTGTTTTGTAGCTACTGTCAGATT AGCTACACCTCAGTTCATCAAGGAAATGTGTACTGTTGAAGAACCAAATGAAGAGTTTACATCTAGACAC AGTTTAGAATGGAAGTTTCTATTTTTAGATCACAGGGCACCACCAATAATAGGCTATTTGCCATTTGAAG TCTTGGGAACATCAGGCTATGATTACTATCATGTGGATGACCTAGAAAATCTGGCAAAATGTCACGAGCA CTTAATGCAATATGGAAAAGGCAAATCGTGTTACTATAGATTCCTGACCAAAGGCCAGCAGTGGATATGG CTTCAGACTCATTATTATATTACTTACCATCAGTGGAATTCAAGGCCAGAGTTCATTGTTTGTACTCACA CTGTAGTAAGTTATGCAGAAGTTAGGGCTGAAAGACGGCGAGAACTTGGCATTGAAGAGTCTCTTCCTGA GACAGCTGCTGACAAAAGCCAAGATTCTGGGTCTGACAATCGTATCAACACAGTGAGTCTCAAGGAAGCA CTGGAAAGGTTTGATCACAGCCCAACTCCTTCTGCCTCCTCTAGAAGCTCACGAAAGTCATCTCACACCG CAGTCTCAGACCCTTCCTCCACACCGACAAAGATCCCTACTGATACTAGCACTCCTCCCAGACAGCATTT GCCAGCTCATGAAAAGATGACACAGCGGAGGTCGTCCTTCAGCAGTCAGTCCATAAACTCCCAGTCAGTT GGTCCATCATTAACACAGCCAGCGATGTCTCAAGCTGCAAATTTACCAATTCCACAAGGCATGTCACAGT TTCAGTTTTCAGCTCAGTTAGGAGCCATGCAGCATCTAAAAGACCAGCTAGAGCAGCGGACACGGATGAT AGAGGCAAATATTCATCGGCAGCAAGAAGAACTAAGGAAAATTCAAGAGCAACTTCAGATGGTCCATGGT CAAGGGCTACAGATGTTTTTGCAGCAATCAAACCCTGGATTGAATTTTGGTTCTGTTCAACTTTCCTCTG GAAATTCTAATATCCAGCAACTCACACCTGTAAATATGCAAGGCCAGGTTGTCCCTGCTAACCAGGTTCA GAGTGGACATATCAGCACAGGCCAGCACATGATACAGCAACAGACTTTACAAAGTACATCAACTCAGCAG AGTCAACAGAGTGTAATGAGTGGACACAGTCAGCAGACGTCTCTTCCAAGTCAGACACCGAGCACTCTCA CAGCCCCACTGTACAATACGATGGTGATTTCCCAGCCTGCAGCTGGGAGCATGGTCCAGATTCCATCCAG TATGCCACAGAACAGTACCCAGAGTGCTACAGTCACTACGTTCACTCAGGACAGACAGATAAGATTTTCT CAAGGTCAGCAACTTGTGACCAAATTAGTGACTGCTCCTGTAGCTTGTGGGGCCGTCATGGTACCAAGTA CCATGCTTATGGGTCAGGTGGTGACTGCCTATCCTACCTTCGCCACACAACAGCAGCAGGCACAGACATT ATCGGTAACACAACAGCAGCAGCAGCAGCAGCAGCAGCCACCACAGCAACAGCAACAACAACAGCAGAGT TCCCAGGAACAGCAGCTTCCTTCAGTTCAGCAGCCAGCTCAGGCCCAGCTGGGCCAGCCACCACAGCAGT TCTTACAGACATCTAGGTTGCTCCACGGGAATCCTTCGACACAGCTCATCCTCTCTGCTGCCTTTCCACT ACAACAGAGCACTTTCCCTCCTTCGCACCACCAGCAACACCAGCCTCAGCAGCAACAGCAGCTTCCTCGG CACAGGACTGACAGCCTGACTGACCCTTCCAAGGTCCAGCCACAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289826
Insert Size	2568 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289826.1, NP_001276755.1
RefSeq Size	9758 bp
RefSeq ORF	2568 bp
Locus ID	12753
UniProt ID	O08785
Gene Summary	The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with Arntl (Bmal1) that binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Polymorphisms in this gene may be associated with behavioral changes, obesity, and metabolic syndrome. Two transcripts encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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