Gria1 (NM_001252403) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2900051M01Rik; Glr-1; Glr1; GluA1; Glur-1; GluR-A; gluR-K1; Glur1; GluRA; HIPA1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231235 representing NM_001252403 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTACCGATTCTGTTCCCAGTTCTCCAAAGGAGTGTACGCCATCTTTGGATTTTATGAACGAAGGA CTGTCAACATGCTGACCTCCTTCTGTGGGGCCCTCCATGTGTGCTTCATCACTCCAAGTTTTCCCGTTGA CACATCCAATCAGTTTGTCCTTCAGCTGCGCCCGGAACTACAGGAAGCTCTCATTAGCATTATCGACCAT TACAAGTGGCAGACTTTTGTCTACATTTATGATGCTGACCGGGGCCTGTCAGTCCTGCAGAGAGTCTTGG ATACAGCCGCCGAGAAGAACTGGCAGGTGACGGCTGTCAACATTCTAACAACCACGGAGGAAGGATACCG GATGCTCTTTCAGGACCTGGAGAAGAAAAAGGAGAGGCTGGTGGTGGTGGACTGTGAATCAGAACGCCTC AACGCCATCCTGGGCCAGATTGTGAAGCTAGAAAAGAACGGCATCGGGTACCACTACATCCTCGCCAACC TGGGCTTCATGGACATTGACTTAAATAAGTTCAAGGAGAGTGGAGCCAATGTGACAGGTTTCCAACTGGT GAACTACACAGACACGATCCCAGCCAGAATCATGCAGCAGTGGAGGACAAGTGACGCTCGGGACCACACC AGGGTGGACTGGAAGAGGCCAAAGTACACTTCTGCTCTTACCTATGATGGTGTGAAGGTGATGGCGGAGG CCTTCCAGAGCCTGCGGAGGCAGAGGATTGACATATCCCGGCGAGGGAATGCTGGGGACTGTCTGGCTAA CCCAGCTGTGCCCTGGGGCCAAGGGATCGACATCCAGAGAGCCCTGCAGCAGGTGCGCTTTGAAGGTTTG ACAGGAAATGTGCAGTTTAACGAGAAAGGGCGCCGGACCAACTACACCCTCCATGTGATCGAAATGAAGC ATGATGGAATCCGCAAGATTGGTTACTGGAATGAAGATGATAAATTTGTCCCCGCAGCCACGGACGCTCA GGCTGGAGGGGACAACTCAAGCGTCCAGAATAGAACCTACATCGTCACGACTATCCTCGAAGATCCTTAC GTGATGCTTAAAAAGAATGCCAACCAATTTGAAGGCAATGACCGCTATGAGGGCTACTGCGTGGAACTGG CTGCGGAGATCGCCAAGCACGTGGGCTATTCCTACCGACTTGAGATTGTCAGCGACGGCAAATACGGAGC CCGGGATCCTGACACAAAGGCCTGGAATGGCATGGTGGGAGAGCTAGTCTATGGAAGAGCAGATGTGGCG GTGGCCCCCTTGACCATAACCTTGGTCCGGGAGGAAGTCATCGACTTCTCCAAGCCATTCATGAGTTTGG GAATCTCCATTATGATTAAGAAGCCACAGAAGTCCAAGCCAGGTGTCTTCTCCTTTCTTGACCCTTTGGC CTACGAGATCTGGATGTGTATAGTGTTTGCCTACATTGGAGTGAGCGTCGTCCTCTTCCTGGTCAGCCGT TTCAGTCCTTATGAATGGCACAGTGAAGAGTTTGAAGAAGGACGAGATCAGACAACCAGTGACCAGTCAA ATGAGTTTGGCATATTCAACAGCCTGTGGTTCTCGCTGGGGGCCTTCATGCAGCAAGGATGTGACATTTC CCCCAGGTCCCTGTCTGGACGCATCGTCGGCGGTGTCTGGTGGTTCTTCACTTTGATTATCATCTCCTCA TACACAGCCAACCTGGCTGCCTTCCTGACTGTGGAAAGGATGGTGTCTCCCATCGAGAGTGCAGAGGACC TGGCAAAGCAGACGGAAATTGCTTATGGGACATTGGAAGCAGGATCCACTAAGGAGTTCTTCAGGAGGTC TAAAATCGCTGTGTTTGAGAAGATGTGGACATACATGAAGTCTGCAGAACCGTCTGTGTTTGTTCGGACC ACAGAGGAGGGCATGATCAGAGTGAGAAAGTCTAAAGGCAAATATGCCTACCTCCTGGAGTCCACCATGA ATGAGTACATTGAGCAACGCAAGCCCTGTGACACCATGAAAGTGGGAGGTAACTTGGATTCCAAAGGCTA TGGCATTGCAACACCCAAGGGGTCCGCCCTGAGAGGTCCCGTAAACCTAGCGGTTTTGAAACTCAGTGAG CAAGGCGTCTTAGACAAGCTGAAAAGCAAATGGTGGTACGATAAAGGGGAATGTGGAAGCAAGGACTCCG GAAGTAAGGACAAGACCAGTGCTCTGAGCCTGAGCAATGTGGCAGGCGTGTTCTACATCCTGATTGGAGG GCTGGGATTGGCCATGCTGGTTGCCTTAATCGAGTTCTGCTACAAATCCCGTAGCGAGTCGAAGCGGATG AAGGGTTTCTGTTTGATTCCACAGCAATCCATCAATGAAGCCATACGGACATCGACCCTCCCCAGGAACA GCGGGGCAGGAGCCAGCGGAGGAAGTGGCAGTGGAGAGAATGGCAGAGTGGTCAGCCAGGACTTCCCCAA GTCCATGCAATCCATTCCCTGCATGAGCCACAGTTCAGGGATGCCCTTGGGAGCCACAGGATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231235 representing NM_001252403 Red=Cloning site Green=Tags(s) MTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDH YKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERL NAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDARDHT RVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGL TGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPY VMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVA VAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSR FSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISS YTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRT TEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRGPVNLAVLKLSE QGVLDKLKSKWWYDKGECGSKDSGSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRM KGFCLIPQQSINEAIRTSTLPRNSGAGASGGSGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252403
ORF Size	2514 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252403.1, NP_001239332.1
RefSeq Size	5382 bp
RefSeq ORF	2517 bp
Locus ID	14799
MW	94.4 kDa
Gene Summary	Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.[UniProtKB/Swiss-Prot Function]

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