Gria1 (NM_001252403) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2900051M01Rik; Glr-1; Glr1; GluA1; Glur-1; GluR-A; gluR-K1; Glur1; GluRA; HIPA1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229024 representing NM_001252403 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTACCGATTCTGTTCCCAGTTCTCCAAAGGAGTGTACGCCATCTTTGGATTTTATGAACGAAGGA CTGTCAACATGCTGACCTCCTTCTGTGGGGCCCTCCATGTGTGCTTCATCACTCCAAGTTTTCCCGTTGA CACATCCAATCAGTTTGTCCTTCAGCTGCGCCCGGAACTACAGGAAGCTCTCATTAGCATTATCGACCAT TACAAGTGGCAGACTTTTGTCTACATTTATGATGCTGACCGGGGCCTGTCAGTCCTGCAGAGAGTCTTGG ATACAGCCGCCGAGAAGAACTGGCAGGTGACGGCTGTCAACATTCTAACAACCACGGAGGAAGGATACCG GATGCTCTTTCAGGACCTGGAGAAGAAAAAGGAGAGGCTGGTGGTGGTGGACTGTGAATCAGAACGCCTC AACGCCATCCTGGGCCAGATTGTGAAGCTAGAAAAGAACGGCATCGGGTACCACTACATCCTCGCCAACC TGGGCTTCATGGACATTGACTTAAATAAGTTCAAGGAGAGTGGAGCCAATGTGACAGGTTTCCAACTGGT GAACTACACAGACACGATCCCAGCCAGAATCATGCAGCAGTGGAGGACAAGTGACGCTCGGGACCACACC AGGGTGGACTGGAAGAGGCCAAAGTACACTTCTGCTCTTACCTATGATGGTGTGAAGGTGATGGCGGAGG CCTTCCAGAGCCTGCGGAGGCAGAGGATTGACATATCCCGGCGAGGGAATGCTGGGGACTGTCTGGCTAA CCCAGCTGTGCCCTGGGGCCAAGGGATCGACATCCAGAGAGCCCTGCAGCAGGTGCGCTTTGAAGGTTTG ACAGGAAATGTGCAGTTTAACGAGAAAGGGCGCCGGACCAACTACACCCTCCATGTGATCGAAATGAAGC ATGATGGAATCCGCAAGATTGGTTACTGGAATGAAGATGATAAATTTGTCCCCGCAGCCACGGACGCTCA GGCTGGAGGGGACAACTCAAGCGTCCAGAATAGAACCTACATCGTCACGACTATCCTCGAAGATCCTTAC GTGATGCTTAAAAAGAATGCCAACCAATTTGAAGGCAATGACCGCTATGAGGGCTACTGCGTGGAACTGG CTGCGGAGATCGCCAAGCACGTGGGCTATTCCTACCGACTTGAGATTGTCAGCGACGGCAAATACGGAGC CCGGGATCCTGACACAAAGGCCTGGAATGGCATGGTGGGAGAGCTAGTCTATGGAAGAGCAGATGTGGCG GTGGCCCCCTTGACCATAACCTTGGTCCGGGAGGAAGTCATCGACTTCTCCAAGCCATTCATGAGTTTGG GAATCTCCATTATGATTAAGAAGCCACAGAAGTCCAAGCCAGGTGTCTTCTCCTTTCTTGACCCTTTGGC CTACGAGATCTGGATGTGTATAGTGTTTGCCTACATTGGAGTGAGCGTCGTCCTCTTCCTGGTCAGCCGT TTCAGTCCTTATGAATGGCACAGTGAAGAGTTTGAAGAAGGACGAGATCAGACAACCAGTGACCAGTCAA ATGAGTTTGGCATATTCAACAGCCTGTGGTTCTCGCTGGGGGCCTTCATGCAGCAAGGATGTGACATTTC CCCCAGGTCCCTGTCTGGACGCATCGTCGGCGGTGTCTGGTGGTTCTTCACTTTGATTATCATCTCCTCA TACACAGCCAACCTGGCTGCCTTCCTGACTGTGGAAAGGATGGTGTCTCCCATCGAGAGTGCAGAGGACC TGGCAAAGCAGACGGAAATTGCTTATGGGACATTGGAAGCAGGATCCACTAAGGAGTTCTTCAGGAGGTC TAAAATCGCTGTGTTTGAGAAGATGTGGACATACATGAAGTCTGCAGAACCGTCTGTGTTTGTTCGGACC ACAGAGGAGGGCATGATCAGAGTGAGAAAGTCTAAAGGCAAATATGCCTACCTCCTGGAGTCCACCATGA ATGAGTACATTGAGCAACGCAAGCCCTGTGACACCATGAAAGTGGGAGGTAACTTGGATTCCAAAGGCTA TGGCATTGCAACACCCAAGGGGTCCGCCCTGAGAGGTCCCGTAAACCTAGCGGTTTTGAAACTCAGTGAG CAAGGCGTCTTAGACAAGCTGAAAAGCAAATGGTGGTACGATAAAGGGGAATGTGGAAGCAAGGACTCCG GAAGTAAGGACAAGACCAGTGCTCTGAGCCTGAGCAATGTGGCAGGCGTGTTCTACATCCTGATTGGAGG GCTGGGATTGGCCATGCTGGTTGCCTTAATCGAGTTCTGCTACAAATCCCGTAGCGAGTCGAAGCGGATG AAGGGTTTCTGTTTGATTCCACAGCAATCCATCAATGAAGCCATACGGACATCGACCCTCCCCAGGAACA GCGGGGCAGGAGCCAGCGGAGGAAGTGGCAGTGGAGAGAATGGCAGAGTGGTCAGCCAGGACTTCCCCAA GTCCATGCAATCCATTCCCTGCATGAGCCACAGTTCAGGGATGCCCTTGGGAGCCACAGGATTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252403
Insert Size	2517 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252403.1, NP_001239332.1
RefSeq Size	5382 bp
RefSeq ORF	2517 bp
Locus ID	14799
Gene Summary	Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and uses a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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