Ap1g1 (NM_001301211) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA409002; Adtg; AU041323; AW551707; D8Ertd374e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231214 representing NM_001301211 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCCCCCATCAGATTGCGGGAGCTGATCCGGACCATCCGGACAGCCCGAACCCAAGCTGAGGAAC GAGAAATGATCCAGAAAGAATGTGCTGCAATCCGGTCCTCATTTAGAGAAGAAGACAATACATACCGGTG TCGGAATGTGGCAAAGTTACTATATATGCACATGCTGGGCTACCCTGCTCACTTTGGACAGTTGGAGTGC CTCAAGCTAATCGCCTCACAAAAATTCACAGACAAACGCATTGGCTATTTAGGAGCAATGCTATTATTAG ATGAAAGACAAGATGTGCATCTTCTCATGACCAACTGTATCAAGAATGATCTTAATCATAGCACGCAGTT TGTGCAGGGGCTAGCACTTTGTACCCTCGGCTGCATGGGCTCCTCGGAGATGTGCAGAGATCTTGCGGGA GAGGTAGAAAAGCTTCTGAAAACCTCCAACTCTTACTTAAGAAAAAAGGCAGCACTGTGTGCTGTTCACG TCATCAGGAAGGTTCCTGAACTTATGGAGATGTTTTTACCAGCAACAAAAAATTTATTGAATGAGAAGAA CCATGGTGTCCTTCACACGTCTGTAGTCCTCCTCACAGAGATGTGTGAACGGAGCCCAGACATGCTCGCA CATTTCAGAAAGCTTGTGCCCCAATTAGTTCGTATTCTAAAGAACCTCATCATGTCCGGATATTCCCCAG AACATGATGTCTCTGGTATCAGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAGAATTTTAGG ACGGAATGACGATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAATACAGAGACT AGTAAAAATGTAGGAAATGCTATTCTGTATGAGACGGTTTTGACTATCATGGATATTAAATCAGAGAGTG GATTGCGAGTTCTAGCCATAAATATTCTTGGTCGATTCTTATTGAACAATGACAAAAATATTAGATATGT TGCTCTGACATCTCTGTTGAAGACTGTGCAGACAGATCATAACGCTGTACAGAGGCATAGAAGCACAATT GTGGACTGTCTGAAAGATCTGGATGTCTCCATAAAAAGACGTGCAATGGAACTGAGTTTTGCCCTGGTGA ATGGGAATAATATCCGAGGCATGATGAAAGAATTACTTTATTTTCTGGATTCCTGTGAGCCAGAATTTAA AGCTGATTGTGCATCTGGAATCTTCCTTGCTGCAGAAAAGTACGCACCTTCCAAACGGTGGCATATAGAT ACAATTATGCGTGTCTTGACAACGGCAGGAAGTTATGTTCGCGATGATGCAGTTCCTAACTTGATTCAGT TAATAACCAACAGTGTGGAGATGCACGCTTACACCGTCCAGCGCCTGTACAAGGCGATTCTCGGTGACTA TTCTCAACAACCCTTGGTACAAGTGGCTGCGTGGTGTATAGGTGAATATGGCGATCTTCTTGTGTCTGGC CAGTGTGAAGAGGAAGAGCCTATTCAGGTGACAGAAGATGAAGTGCTGGATATTTTAGAAAGTGTCCTGA TCTCTAATATGTCCACCTCTGTGACAAGAGGCTATGCTCTCACTGCCATTATGAAGCTGTCTACCCGATT CACCTGTACTGTAAACCGAATTAAGAAAGTGGTTTCCATCTATGGGAGCAGCATCGACGTAGAGCTCCAG CAGAGGGCAGTAGAGTACAACGCACTTTTTAAGAAGTATGACCACATGAGGTCTGCCCTACTTGAAAGAA TGCCTGTCATGGAAAAAGTGACCACAAATGGCCCTTCGGAGATCGTGCAGACAAATGGAGAGACAGAACC AGCGCCCCTAGAGACTAAACCACCACCCTCAGGGCCACAACCCACAAGCCAGGCCAATGATCTATTGGAT TTGTTGGGAGGAAATGACATAACACCTGTTATCCCAACTGCACCTACAAGCAAACCAGCATCAGCTGGTG GAGAACTTCTCGACTTGCTAGGAGACATCACCCTGACAGGTGCTCCAGCTGCTGCTCCTACCCCTGCCTC AGTGCCACAGATATCCCAGCCCCCCTTCTTGTTGGATGGGCTTTCCTCACAGCCTCTCTTCAATGACATC GCTCCAGGCATCCCCTCCATCACAGCGTACAGTAAGAACGGCTTGAAGATAGAGTTCACCTTTGAACGGT CAAACACCAACCCCAGCGTAACAGTGATAACGATACAGGCCTCTAACAGCACAGAGCTAGACATGACGGA CTTTGTTTTCCAGGCTGCAGTACCAAAGACATTCCAGCTGCAGCTTCTGTCTCCTAGCAGCAGCGTTGTC CCAGCCTTTAATACAGGGACCATCACACAAGTCATTAAAGTTCTGAATCCACAGAAGCAACAGCTGCGAA TGCGGATCAAGCTCACATATAATCACAAGGGCTCGGCAATGCAAGATCTAGCAGAAGTGAACAATTTCCC CCCTCAGTCCTGGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231214 representing NM_001301211 Red=Cloning site Green=Tags(s) MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLGYPAHFGQLEC LKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAG EVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATKNLLNEKNHGVLHTSVVLLTEMCERSPDMLA HFRKLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATNTET SKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHRSTI VDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRWHID TIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCIGEYGDLLVSG QCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQ QRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPSEIVQTNGETEPAPLETKPPPSGPQPTSQANDLLD LLGGNDITPVIPTAPTSKPASAGGELLDLLGDITLTGAPAAAPTPASVPQISQPPFLLDGLSSQPLFNDI APGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSVV PAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301211
ORF Size	2466 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301211.1, NP_001288140.1
RefSeq Size	6882 bp
RefSeq ORF	2469 bp
Locus ID	11765
UniProt ID	P22892
MW	91.8 kDa
Gene Summary	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.[UniProtKB/Swiss-Prot Function]

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