Spag16 (NM_001271533) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4921511D23Rik; 4930524F24Rik; 4930585K05Rik; AV261009; Pf20; Wdr29
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229744 representing NM_001271533 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTCCGTCTGGGGTCCCGCCGCTGCGGGTGCTGGAGGAGCTGGGCATTGGCTTGTCACCCACCG GCGAGGTGACAGAGGCGGTGACCTCCGAGGGCGCCTACTACCTGGAGCAGGTCACCATAACGGAAACATC GGAAGATGAATGCGAATATGAAGAGATACCAGATGACAATTTCAGCATTCCTGAAGGAGAAGAAGATCTG GAAAAAGCAATTCACATCATTGGAGAGCAAGCCAGAGACATCCATATTTTGGAACAACAAACCATTCTTC CTGCAAGGAATGTAATGCAGGAAGCCATAGAAGATTTTCTCTGCAATTTCCTGATCAAAATGGGGATGAC CAGAACTCTGGACTGCTTCCAGGCCGAGTGGTATGAGTTAATACAGAAGAAAGGAAGTGACTTTAAAGGG CTCGGTAATGTTCCCGATGTCTACAGCCAGGTGATGCTTTTAGAGACTGAGAACAAAAACTTGAAGAAGG AATTGAAGCACTTCAAACAGGCAGCTGAAAAGGCCAAAGAAGATCTACTGAGAACTCAGAAGGAACGGGA CTTCCATCGCATGCATCATAAGCGCATAGTGCAAGAAAAGAACAAGCTCATTGCTGACCTCAAAGGGCTG AAATTGCATTATGCATCATACGAACCAACTATAAGGGTGTTACATGAGAAGCATCATGCTTTATTGAAGG AGAAAATGCTGACCTCTTTGGAAAGAGATCGAGCTGTTGGGAAGATTTCTGGGCTTCAAGCAACACTGAA GAACATAGACATGGGACATATTCAGGTTCCTGTAATTAAAGGATCTTATGAGAGTGCTTCTATCACAAGA GAGTCAGGAGACAGAGCTGGTCACAGTTGTGAGAAAGAAAACAGTTCAGAGGGCCCAACTCAGAAAAGTC TTCGTGAAGCCAGGGAAGAAGTTGGATATAAATCAAAGTTGAAGAATGAGAAAAAGCCATCTTCAACAGA GTCCAAGAGGGTCCAACCGAGTCCAACAGAGTCTCATATGGTCAGTTCACGATCATCATCGCTCTACCCA ACTCCTTCCTTCCATCAGCAGCAGTCAAGAGGGAACTTTCCAACTTGGAATAAGATCACACCTCAACAGG GCTCCATTAGCACAAGAGAGCAAAGTACAGGGACACTCAGAAAAAGACCACCGAAGACCTCTTGTCTCCA TCAGCATGAGTCAAATGGAAGATTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229744 representing NM_001271533 Red=Cloning site Green=Tags(s) MAAPSGVPPLRVLEELGIGLSPTGEVTEAVTSEGAYYLEQVTITETSEDECEYEEIPDDNFSIPEGEEDL EKAIHIIGEQARDIHILEQQTILPARNVMQEAIEDFLCNFLIKMGMTRTLDCFQAEWYELIQKKGSDFKG LGNVPDVYSQVMLLETENKNLKKELKHFKQAAEKAKEDLLRTQKERDFHRMHHKRIVQEKNKLIADLKGL KLHYASYEPTIRVLHEKHHALLKEKMLTSLERDRAVGKISGLQATLKNIDMGHIQVPVIKGSYESASITR ESGDRAGHSCEKENSSEGPTQKSLREAREEVGYKSKLKNEKKPSSTESKRVQPSPTESHMVSSRSSSLYP TPSFHQQQSRGNFPTWNKITPQQGSISTREQSTGTLRKRPPKTSCLHQHESNGRF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271533
ORF Size	1215 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271533.1, NP_001258462.1
RefSeq Size	3589 bp
RefSeq ORF	1218 bp
Locus ID	66722
UniProt ID	Q8K450
MW	46.4 kDa
Gene Summary	Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.