Spag16 (NM_001271533) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4921511D23Rik; 4930524F24Rik; 4930585K05Rik; AV261009; Pf20; Wdr29
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227533 representing NM_001271533 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTCCGTCTGGGGTCCCGCCGCTGCGGGTGCTGGAGGAGCTGGGCATTGGCTTGTCACCCACCG GCGAGGTGACAGAGGCGGTGACCTCCGAGGGCGCCTACTACCTGGAGCAGGTCACCATAACGGAAACATC GGAAGATGAATGCGAATATGAAGAGATACCAGATGACAATTTCAGCATTCCTGAAGGAGAAGAAGATCTG GAAAAAGCAATTCACATCATTGGAGAGCAAGCCAGAGACATCCATATTTTGGAACAACAAACCATTCTTC CTGCAAGGAATGTAATGCAGGAAGCCATAGAAGATTTTCTCTGCAATTTCCTGATCAAAATGGGGATGAC CAGAACTCTGGACTGCTTCCAGGCCGAGTGGTATGAGTTAATACAGAAGAAAGGAAGTGACTTTAAAGGG CTCGGTAATGTTCCCGATGTCTACAGCCAGGTGATGCTTTTAGAGACTGAGAACAAAAACTTGAAGAAGG AATTGAAGCACTTCAAACAGGCAGCTGAAAAGGCCAAAGAAGATCTACTGAGAACTCAGAAGGAACGGGA CTTCCATCGCATGCATCATAAGCGCATAGTGCAAGAAAAGAACAAGCTCATTGCTGACCTCAAAGGGCTG AAATTGCATTATGCATCATACGAACCAACTATAAGGGTGTTACATGAGAAGCATCATGCTTTATTGAAGG AGAAAATGCTGACCTCTTTGGAAAGAGATCGAGCTGTTGGGAAGATTTCTGGGCTTCAAGCAACACTGAA GAACATAGACATGGGACATATTCAGGTTCCTGTAATTAAAGGATCTTATGAGAGTGCTTCTATCACAAGA GAGTCAGGAGACAGAGCTGGTCACAGTTGTGAGAAAGAAAACAGTTCAGAGGGCCCAACTCAGAAAAGTC TTCGTGAAGCCAGGGAAGAAGTTGGATATAAATCAAAGTTGAAGAATGAGAAAAAGCCATCTTCAACAGA GTCCAAGAGGGTCCAACCGAGTCCAACAGAGTCTCATATGGTCAGTTCACGATCATCATCGCTCTACCCA ACTCCTTCCTTCCATCAGCAGCAGTCAAGAGGGAACTTTCCAACTTGGAATAAGATCACACCTCAACAGG GCTCCATTAGCACAAGAGAGCAAAGTACAGGGACACTCAGAAAAAGACCACCGAAGACCTCTTGTCTCCA TCAGCATGAGTCAAATGGAAGATTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271533
Insert Size	1218 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271533.1, NP_001258462.1
RefSeq Size	3589 bp
RefSeq ORF	1218 bp
Locus ID	66722
UniProt ID	Q8K450
Gene Summary	Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks several exons and includes an alternate 3' terminal exon compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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