Eln (NM_007925) Mouse Tagged ORF Clone
CAT#: MR227515
- TrueORF®
Eln (Myc-DDK-tagged) - Mouse elastin (Eln)
ORF Plasmid: tGFP
"NM_007925" in other vectors (4)
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CNY 6,296.00
CNY 7,130.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI385707; AI480567; E030024M20Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR227515 representing NM_007925
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGTCTGACAGCGGTAGTCCCGCAGCCTGGCGTCTTGCTGATCCTCTTGCTCAACCTCCTCCATC CCGCGCAGCCTGGAGGGGTTCCAGGAGCTGTGCCTGGCGGACTTCCTGGTGGAGTTCCCGGTGGAGTCTA TTATCCAGGGGCTGGTATTGGAGGCCTGGGAGGAGGAGGAGGAGCTCTGGGACCTGGAGGAAAACCACCT AAGCCAGGTGCCGGACTTCTGGGAACGTTTGGAGCAGGTCCTGGAGGACTTGGAGGTGCTGGCCCGGGTG CAGGTCTCGGGGCCTTTCCTGCAGGCACCTTCCCAGGGGCAGGAGCTCTGGTGCCCGGGGGAGCAGCAGG GGCTGCTGCGGCTTATAAAGCTGCCGCCAAAGCTGGGGCTGGGCTTGGTGGCGTTGGCGGAGTCCCAGGT GGTGTTGGCGTTGGTGGAGTTCCAGGTGGTGTTGGAGTTGGCGGAGTCCCAGGTGGTGTTGGAGTTGGTG GAGTCCCTGGCGGTGTTGGTGGTATTGGTGGCATCGGTGGCTTAGGAGTCTCGACAGGTGCTGTGGTGCC CCAAGTCGGAGCTGGCATCGGAGCTGGAGGAAAGCCTGGGAAAGTTCCTGGTGTTGGTCTTCCAGGTGTA TACCCAGGCGGAGTGCTCCCAGGAACAGGAGCTCGGTTCCCTGGTGTGGGGGTGCTCCCTGGAGTTCCCA CTGGCACAGGAGTCAAAGCCAAGGCTCCAGGTGGAGGTGGTGCTTTTGCTGGAATCCCAGGGGTCGGACC CTTTGGGGGTCAGCAGCCTGGTGTCCCACTGGGTTATCCCATCAAAGCACCAAAGCTGCCAGGTGGCTAC GGACTGCCCTATACCAATGGGAAATTGCCCTATGGAGTAGCTGGTGCAGGGGGCAAGGCTGGCTACCCAA CAGGGACAGGGGTCGGATCCCAGGCGGCGGCGGCAGCAGCTAAAGCAGCCAAGTATGGTGCTGGGGGAGC TGGAGTCCTCCCTGGTGTTGGAGGGGGTGGCATTCCTGGTGGTGCTGGCGCAATTCCTGGAATTGGAGGC ATTGCAGGCGCTGGAACTCCTGCAGCAGCAGCTGCTGCAAAGGCTGCTGCTAAGGCTGCTAAGTATGGAG CTGCTGGAGGTTTAGTGCCTGGTGGACCAGGAGTTAGGCTCCCAGGTGCTGGAATCCCAGGTGTTGGTGG CATTCCTGGTGTTGGTGGCATCCCAGGTGTTGGGGGCCCTGGTATTGGAGGTCCAGGCATTGTGGGTGGA CCAGGAGCTGTGTCACCAGCTGCAGCTGCTAAAGCTGCTGCCAAAGCTGCCAAATACGGAGCCAGAGGTG GAGTTGGCATCCCGACATATGGGGTTGGTGCTGGTGGCTTTCCTGGCTATGGTGTTGGAGCTGGAGCAGG ACTTGGAGGTGCAAGCCCAGCTGCTGCTGCTGCCGCCGCCAAAGCTGCTAAGTATGGTGCTGGAGGAGCT GGAGCCCTGGGAGGCCTGGTGCCAGGTGCAGTACCAGGTGCACTGCCAGGTGCAGTACCAGCTGTGCCGG GAGCTGGTGGAGTGCCAGGAGCAGGTACCCCTGCAGCTGCAGCTGCTGCCGCCGCCGCTAAAGCAGCCGC CAAAGCAGGTTTGGGTCCTGGTGTTGGTGGGGTTCCTGGTGGAGTTGGTGTTGGTGGGATTCCCGGTGGA GTTGGTGTTGGTGGGGTTCCTGGTGGAGTTGGCCCTGGTGGTGTTACTGGTATTGGAGCTGGTCCTGGCG GTCTTGGAGGAGCAGGGTCACCGGCTGCCGCTAAATCTGCTGCTAAGGCAGCTGCCAAAGCCCAGTACAG AGCTGCCGCTGGGCTTGGAGCTGGTGTCCCTGGATTTGGGGCTGGTGCTGGTGTCCCCGGATTTGGGGCT GGTGCTGGTGTCCCCGGATTTGGGGCTGGTGCTGGTGTCCCCGGATTTGGGGCTGGTGCTGGTGTCCCTG GATTTGGAGCTGGAGCAGTACCTGGATCGCTGGCTGCATCCAAAGCTGCTAAATATGGAGCAGCAGGTGG CCTTGGTGGCCCTGGAGGTCTCGGTGGCCCTGGAGGTCTCGGTGGACCTGGAGGACTTGGTGGGGCTGGT GTTCCCGGTAGAGTAGCAGGAGCTGCACCCCCTGCTGCTGCCGCTGCTGCTGCCAAAGCTGCTGCTAAGG CTGCCCAGTATGGCCTTGGTGGAGCCGGAGGATTGGGAGCCGGTGGACTGGGGGCCGGTGGACTGGGAGC CGGTGGACTGGGAGCTGGTGGACTGGGAGCCGGTGGACTGGGAGCTGGTGGACTGGGAGCCGGTGGACTG GGAGCTGGTGGAGGTGTGTCCCCTGCTGCAGCTGCTAAGGCAGCCAAATATGGTGCTGCTGGCCTTGGAG GTGTCCTAGGAGCCAGGCCATTCCCAGGTGGAGGAGTTGCAGCAAGACCTGGCTTTGGACTTTCTCCCAT TTATCCAGGTGGTGGTGCTGGGGGCCTGGGAGTTGGTGGAAAACCCCCGAAGCCCTATGGAGGAGCCCTT GGAGCCCTGGGATACCAAGGTGGGGGCTGCTTTGGGAAATCCTGTGGCCGGAAGAGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227515 representing NM_007925
Red=Cloning site Green=Tags(s) MAGLTAVVPQPGVLLILLLNLLHPAQPGGVPGAVPGGLPGGVPGGVYYPGAGIGGLGGGGGALGPGGKPP KPGAGLLGTFGAGPGGLGGAGPGAGLGAFPAGTFPGAGALVPGGAAGAAAAYKAAAKAGAGLGGVGGVPG GVGVGGVPGGVGVGGVPGGVGVGGVPGGVGGIGGIGGLGVSTGAVVPQVGAGIGAGGKPGKVPGVGLPGV YPGGVLPGTGARFPGVGVLPGVPTGTGVKAKAPGGGGAFAGIPGVGPFGGQQPGVPLGYPIKAPKLPGGY GLPYTNGKLPYGVAGAGGKAGYPTGTGVGSQAAAAAAKAAKYGAGGAGVLPGVGGGGIPGGAGAIPGIGG IAGAGTPAAAAAAKAAAKAAKYGAAGGLVPGGPGVRLPGAGIPGVGGIPGVGGIPGVGGPGIGGPGIVGG PGAVSPAAAAKAAAKAAKYGARGGVGIPTYGVGAGGFPGYGVGAGAGLGGASPAAAAAAAKAAKYGAGGA GALGGLVPGAVPGALPGAVPAVPGAGGVPGAGTPAAAAAAAAAKAAAKAGLGPGVGGVPGGVGVGGIPGG VGVGGVPGGVGPGGVTGIGAGPGGLGGAGSPAAAKSAAKAAAKAQYRAAAGLGAGVPGFGAGAGVPGFGA GAGVPGFGAGAGVPGFGAGAGVPGFGAGAVPGSLAASKAAKYGAAGGLGGPGGLGGPGGLGGPGGLGGAG VPGRVAGAAPPAAAAAAAKAAAKAAQYGLGGAGGLGAGGLGAGGLGAGGLGAGGLGAGGLGAGGLGAGGL GAGGGVSPAAAAKAAKYGAAGLGGVLGARPFPGGGVAARPGFGLSPIYPGGGAGGLGVGGKPPKPYGGAL GALGYQGGGCFGKSCGRKRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007925 |
ORF Size | 2580 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007925.1, NM_007925.2, NM_007925.3, NM_007925.4, NP_031951.2 |
RefSeq Size | 3781 bp |
RefSeq ORF | 2583 bp |
Locus ID | 13717 |
UniProt ID | P54320 |
MW | 72.4 kDa |
Gene Summary | This gene encodes elastin, the extracellular matrix protein that forms a major structural component of several tissues including lungs and arterial walls. Cleavage of the signal peptide from the encoded precursor generates soluble tropoelastin which undergoes lysine-derived crosslinking to form elastin polymers. Mice lacking the encoded protein exhibit defective lung development, and die of an obstructive arterial disease resulting from subendothelial cell proliferation and reorganization of smooth muscle. [provided by RefSeq, Aug 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222143 | Eln (untagged) - Mouse elastin (Eln), (10ug) |
CNY 10,930.00 |
|
MG227515 | Eln (tGFP-tagged) - Mouse elastin (Eln), (10ug) |
CNY 7,890.00 |
|
MR227515L3 | Lenti ORF clone of Eln (Myc-DDK-tagged) - Mouse elastin (Eln) |
CNY 9,030.00 |
|
MR227515L4 | Lenti ORF clone of Eln (mGFP-tagged) - Mouse elastin (Eln) |
CNY 9,030.00 |