Eln (NM_007925) Mouse Untagged Clone

CAT#: MC222143

Eln (untagged) - Mouse elastin (Eln), (10ug)



  "NM_007925" in other vectors (4)

CNY 10,930.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI385707; AI480567; E030024M20Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222143 representing NM_007925
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGTCTGACAGCGGTAGTCCCGCAGCCTGGCGTCTTGCTGATCCTCTTGCTCAACCTCCTCCATC
CCGCGCAGCCTGGAGGGGTTCCAGGAGCTGTGCCTGGCGGACTTCCTGGTGGAGTTCCCGGTGGAGTCTA
TTATCCAGGGGCTGGTATTGGAGGCCTGGGAGGAGGAGGAGGAGCTCTGGGACCTGGAGGAAAACCACCT
AAGCCAGGTGCCGGACTTCTGGGAACGTTTGGAGCAGGTCCTGGAGGACTTGGAGGTGCTGGCCCGGGTG
CAGGTCTCGGGGCCTTTCCTGCAGGCACCTTCCCAGGGGCAGGAGCTCTGGTGCCCGGGGGAGCAGCAGG
GGCTGCTGCGGCTTATAAAGCTGCCGCCAAAGCTGGGGCTGGGCTTGGTGGCGTTGGCGGAGTCCCAGGT
GGTGTTGGCGTTGGTGGAGTTCCAGGTGGTGTTGGAGTTGGCGGAGTCCCAGGTGGTGTTGGAGTTGGTG
GAGTCCCTGGCGGTGTTGGTGGTATTGGTGGCATCGGTGGCTTAGGAGTCTCGACAGGTGCTGTGGTGCC
CCAAGTCGGAGCTGGCATCGGAGCTGGAGGAAAGCCTGGGAAAGTTCCTGGTGTTGGTCTTCCAGGTGTA
TACCCAGGCGGAGTGCTCCCAGGAACAGGAGCTCGGTTCCCTGGTGTGGGGGTGCTCCCTGGAGTTCCCA
CTGGCACAGGAGTCAAAGCCAAGGCTCCAGGTGGAGGTGGTGCTTTTGCTGGAATCCCAGGGGTCGGACC
CTTTGGGGGTCAGCAGCCTGGTGTCCCACTGGGTTATCCCATCAAAGCACCAAAGCTGCCAGGTGGCTAC
GGACTGCCCTATACCAATGGGAAATTGCCCTATGGAGTAGCTGGTGCAGGGGGCAAGGCTGGCTACCCAA
CAGGGACAGGGGTCGGATCCCAGGCGGCGGCGGCAGCAGCTAAAGCAGCCAAGTATGGTGCTGGGGGAGC
TGGAGTCCTCCCTGGTGTTGGAGGGGGTGGCATTCCTGGTGGTGCTGGCGCAATTCCTGGGATTGGAGGC
ATTGCAGGCGCTGGAACTCCTGCAGCAGCAGCTGCTGCAAAGGCTGCTGCTAAGGCTGCTAAGTATGGAG
CTGCTGGAGGTTTAGTGCCTGGTGGACCAGGAGTTAGGCTCCCAGGTGCTGGAATCCCAGGTGTTGGTGG
CATTCCTGGTGTTGGTGGCATCCCAGGTGTTGGGGGCCCTGGTATTGGAGGTCCAGGCATTGTGGGTGGA
CCAGGAGCTGTGTCACCAGCTGCAGCTGCTAAAGCTGCTGCCAAAGCTGCCAAATACGGAGCCAGAGGTG
GAGTTGGCATCCCGACATATGGGGTTGGTGCTGGTGGCTTTCCTGGCTATGGTGTTGGAGCTGGAGCAGG
ACTTGGAGGTGCAAGCCCAGCTGCTGCTGCTGCCGCCGCCAAAGCTGCTAAGTATGGTGCTGGAGGAGCT
GGAGCCCTGGGAGGCCTGGTGCCAGGTGCAGTACCAGGTGCACTGCCAGGTGCAGTACCAGCTGTGCCGG
GAGCTGGTGGAGTGCCAGGAGCAGGTACCCCTGCAGCTGCAGCTGCTGCCGCCGCCGCTAAAGCAGCCGC
CAAAGCAGGTTTGGGTCCTGGTGTTGGTGGGGTTCCTGGTGGAGTTGGTGTTGGTGGGATTCCCGGTGGA
GTTGGTGTTGGTGGGGTTCCTGGTGGAGTTGGCCCTGGTGGTGTTACTGGTATTGGAGCTGGTCCTGGCG
GTCTTGGAGGAGCAGGGTCACCGGCTGCCGCTAAATCTGCTGCTAAGGCAGCTGCCAAAGCCCAGTACAG
AGCTGCCGCTGGGCTTGGAGCTGGTGTCCCTGGATTTGGGGCTGGTGCTGGTGTCCCCGGATTTGGGGCT
GGTGCTGGTGTCCCCGGATTTGGGGCTGGTGCTGGTGTCCCCGGATTTGGGGCTGGTGCTGGTGTCCCTG
GATTTGGAGCTGGAGCAGTACCTGGATCGCTGGCTGCATCCAAAGCTGCTAAATATGGAGCAGCAGGTGG
CCTTGGTGGCCCTGGAGGTCTCGGTGGCCCTGGAGGTCTCGGTGGACCTGGAGGACTTGGTGGGGCTGGT
GTTCCCGGTAGAGTAGCAGGAGCTGCACCCCCTGCTGCTGCCGCTGCTGCTGCCAAAGCTGCTGCTAAGG
CTGCCCAGTATGGCCTTGGTGGAGCCGGAGGATTGGGAGCCGGTGGACTGGGGGCCGGTGGACTGGGAGC
CGGTGGACTGGGAGCTGGTGGACTGGGAGCCGGTGGACTGGGAGCTGGTGGACTGGGAGCCGGTGGACTG
GGAGCTGGTGGAGGTGTGTCCCCTGCTGCAGCTGCTAAGGCAGCCAAATATGGTGCTGCTGGCCTTGGAG
GTGTCCTAGGAGCCAGGCCATTCCCAGGTGGAGGAGTTGCAGCAAGACCTGGCTTTGGACTTTCTCCCAT
TTATCCAGGTGGTGGTGCTGGGGGCCTGGGAGTTGGTGGAAAACCCCCGAAGCCCTATGGAGGAGCCCTT
GGAGCCCTGGGATACCAAGGTGGGGGCTGCTTTGGGAAATCCTGTGGCCGGAAGAGAAAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_007925
Insert Size 2583 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007925.4, NP_031951.2
RefSeq Size 3865 bp
RefSeq ORF 2583 bp
Locus ID 13717
UniProt ID P54320
Gene Summary This gene encodes elastin, the extracellular matrix protein that forms a major structural component of several tissues including lungs and arterial walls. Cleavage of the signal peptide from the encoded precursor generates soluble tropoelastin which undergoes lysine-derived crosslinking to form elastin polymers. Mice lacking the encoded protein exhibit defective lung development, and die of an obstructive arterial disease resulting from subendothelial cell proliferation and reorganization of smooth muscle. [provided by RefSeq, Aug 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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