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Usp30 (NM_001033202) Mouse Tagged Lenti ORF Clone
CAT＃: MR226441L4
- LentiORF®
Lenti ORF clone of Usp30 (mGFP-tagged) - Mouse ubiquitin specific peptidase 30 (Usp30)
"NM_001033202" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | 6330590F17Rik; AI851327; D5Ertd483e |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR226441).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033202 |
| ORF Size | 1551 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033202.3, NP_001028374.1 |
| RefSeq Size | 2790 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 100756 |
| UniProt ID | Q3UN04 |
| Gene Summary | Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (By similarity). Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (PubMed:24513856).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217339 | Usp30 (untagged) - Mouse ubiquitin specific peptidase 30 (Usp30), (10ug) |
CNY 6,650.00 |
|
| MG226441 | Usp30 (tGFP-tagged) - Mouse ubiquitin specific peptidase 30 (Usp30), (10ug) |
CNY 5,448.00 |
|
| MR226441 | Usp30 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 30 (Usp30) |
CNY 3,848.00 |
|
| MR226441L3 | Lenti ORF clone of Usp30 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 30 (Usp30) |
CNY 6,370.00 |
