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Dmrt1 (NM_015826) Mouse Tagged ORF Clone
CAT＃: MR225528
- TrueORF®
Dmrt1 (Myc-DDK-tagged) - Mouse doublesex and mab-3 related transcription factor 1 (Dmrt1)
ORF Plasmid: tGFP
"NM_015826" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225528 representing NM_015826
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAACGACGACACATTCGGCAAGCCCTCTACCCCGACGGAGGTTCCTCACGCTCCCGGGGCCCCGC CGCAGGGCAAAGCCGGAGGTTACAGCAAAGCTGCCGGGGCGATGGCTGGAGCGGCTGGAGGCTCGGGCGC GGGGGGCAGCGGAGGTGCCTCGGGCTCCGGGCCGTCGGGCCTGGGTTCTGGAAGCAAGAAGTCCCCGAGG CTGCCCAAATGCGCTCGCTGCAGGAACCACGGCTACGCGTCGCCGCTCAAGGGCCACAAGCGCTTCTGCA TGTGGCGGGATTGCCAGTGCAAGAAGTGCAGCCTGATTGCGGAGCGACAGCGGGTGATGGCCGCGCAGGT GGCCCTGAGAAGACAGCAGGCCCAGGAAGAAGAACTGGGTATCAGCCACCCAATTCCGCTGCCCAGCGCA GCCGAGCTCCTGGTCAAAAGAGAGAATAATGCCAGCAACCCGTGCCTGATGGCCGAGAACAGCAGCTCTG CCCAGCCCCCACCGGCCAGCACCCCAACCCCCGCTGCCTCAGAGGGACGCATGGTCATCCAGGATATTCC TGCTGTCACCAGCAGAGGGCATATGGAGAACACATCTGACCTGGTATCAGACCCCGCCTACTACAGCAGC TTTTACCAGCCTTCTCTGTTTCCTTACTACAACAATCTGTACAACTACCCCCAGTATTCCATGGCCTTGT CTGCTGAGTCCTCCTCAGGGGAGGTGGGGAATTCCCTCGGGGGATCCCCCGTGAAGAACAGCCTGCGGAG TCTCCCAGCACCTTACGTGCCTGCTCAGACTGGAAACCAGTGGCAGATGAAGACCTCAGAGAGCCGCCAC CCAGTGAGCTCCCAGTACCGGATGCACTCATACTACGGGCCTCCCTCCTACCTGGGCCAGAGCATGTCCC AGATCTTTACCTTCGAGGAAGGCCCCTCCTACTCAGAAGCCAAAGCCAGTGTGTTCTCGCCCCCCAGCAG TCAAGATTCTGGCTTGGTCTCCCTCTCCAGCAGCTCTCCGATGAGCAACGAGAGCTCGAAGGGAGTTCTG GAATGTGAGTCTGCGTCCTCGGAACCTAGCAGCTACGCCGTCAACCAGGTCCTGGAAGAGGATGAGGACG AG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR225528 representing NM_015826
Red=Cloning site Green=Tags(s) MPNDDTFGKPSTPTEVPHAPGAPPQGKAGGYSKAAGAMAGAAGGSGAGGSGGASGSGPSGLGSGSKKSPR LPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCSLIAERQRVMAAQVALRRQQAQEEELGISHPIPLPSA AELLVKRENNASNPCLMAENSSSAQPPPASTPTPAASEGRMVIQDIPAVTSRGHMENTSDLVSDPAYYSS FYQPSLFPYYNNLYNYPQYSMALSAESSSGEVGNSLGGSPVKNSLRSLPAPYVPAQTGNQWQMKTSESRH PVSSQYRMHSYYGPPSYLGQSMSQIFTFEEGPSYSEAKASVFSPPSSQDSGLVSLSSSSPMSNESSKGVL ECESASSEPSSYAVNQVLEEDEDE SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015826 |
| ORF Size | 1122 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015826.5, NP_056641.2 |
| RefSeq Size | 2251 bp |
| RefSeq ORF | 1125 bp |
| Locus ID | 50796 |
| UniProt ID | Q9QZ59 |
| MW | 39.9 kDa |
| Gene Summary | Transcription factor that plays a key role in male sex determination and differentiation by controlling testis development and male germ cell proliferation. Plays a central role in spermatogonia by inhibiting meiosis in undifferentiated spermatogonia and promoting mitosis, leading to spermatogonial development and allowing abundant and continuous production of sperm. Acts both as a transcription repressor and activator: prevents meiosis by restricting retinoic acid (RA)-dependent transcription and repressing STRA8 expression and promotes spermatogonial development by activating spermatogonial differentiation genes, such as SOHLH1. Also plays a key role in postnatal sex maintenance by maintaining testis determination and preventing feminization: represses transcription of female promoting genes such as FOXL2 and activates male-specific genes. May act as a tumor suppressor. May also play a minor role in oogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209870 | Dmrt1 (untagged) - Mouse doublesex and mab-3 related transcription factor 1 (Dmrt1), (10ug) |
CNY 3,656.00 |
|
| MG225528 | Dmrt1 (tGFP-tagged) - Mouse doublesex and mab-3 related transcription factor 1 (Dmrt1), (10ug) |
CNY 3,140.00 |
|
| MR225528L3 | Lenti ORF clone of Dmrt1 (Myc-DDK-tagged) - Mouse doublesex and mab-3 related transcription factor 1 (Dmrt1) |
CNY 4,750.00 |
|
| MR225528L4 | Lenti ORF clone of Dmrt1 (mGFP-tagged) - Mouse doublesex and mab-3 related transcription factor 1 (Dmrt1) |
CNY 4,750.00 |
