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Asah2 (NM_018830) Mouse Tagged ORF Clone
CAT＃: MR224900
- TrueORF®
Asah2 (Myc-DDK-tagged) - Mouse N-acylsphingosine amidohydrolase 2 (Asah2)
ORF Plasmid: tGFP
"NM_018830" in other vectors (4)
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CNY 8,304.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI585898 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224900 representing NM_018830
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGCGAACCTTCTCCACCTTGGAGGCATTCCTCATTTTCCTTCTGGTAATAATGACAGTCATCA CAGTGGCCCTTCTCACCCTCTTGTTTGTTACCAGTGGGACCATTGAAAACCACAAAGATTCAGGAAATCA CTGGTTTTCAACCACTCTGGGCTCCACGACAACCCAGCCCCCTCCAATTACACAGACTCCAAACTTCCCT TCATTTCGGAACTTCAGTGGCTACTACATTGGCGTTGGGAGAGCAGATTGCACAGGACAAGTGTCAGATA TCAATTTGATGGGCTATGGCAAAAATGGCCAGAATGCACGGGGTCTCCTCACCAGGCTGTTCAGCCGTGC TTTTATCTTGGCGGATCCAGATGGGTCAAATCGAATGGCATTTGTGAGCGTGGAACTATGTATGATTTCC CAACGACTGAGGTTGGAGGTCCTGAAGAGACTAGAGAGTAAATATGGCTCTCTGTATCGAAGAGACAATG TTATCCTGAGTGCCATTCACACACACTCTGGCCCAGCAGGGTTTTTCCAATATACACTCTATATACTCGC CAGCGAGGGATTCAGCAACCGGACCTTTCAGTACATAGTCTCTGGGATCATGAAGAGCATTGATATAGCT CACACAAATCTTAAACCAGGCAAAATCTTTATCAACAAAGGAAATGTTGCTAATGTGCAGATCAACCGAA GCCCCTCCTCTTACCTTCTGAATCCACAGTCAGAGAGAGCAAGGTATTCTTCAAACACAGACAAGGAAAT GCTGGTCTTGAAACTGGTGGATTTGAATGGAGAAGACTTGGGTCTTATCAGCTGGTTTGCCATCCACCCC GTGAGCATGAACAATAGCAACCACTTTGTGAATAGTGACAATATGGGCTATGCGGCTTACCTTTTTGAGC AAGAAAAGAACAAAGGCTATCTGCCTGGACAGGGACCGTTTGTAGCAGGCTTTGCTTCATCAAATCTCGG AGACGTGTCACCCAACATTCTTGGCCCGCATTGTGTCAACACAGGGGAGTCTTGTGACAACGACAAGAGC ACCTGTCCCAACGGTGGGCCTAGCATGTGCATGGCCAGCGGACCTGGACAAGACATGTTTGAGAGCACAC ACATTATAGGACGGATCATCTATCAGAAGGCCAAGGAGCTGTATGCCTCTGCCTCCCAGGAGGTGACCGG CCCAGTGCTTGCAGCTCACCAGTGGGTGAACATGACAGATGTGAGCGTCCAGCTCAATGCCACACACACA GTGAAGACGTGTAAACCTGCCCTGGGCTACAGTTTTGCCGCAGGCACAATTGATGGAGTTTCGGGCCTCA ATATTACACAGGGAACTACGGAAGGGGATCCATTCTGGGACACTCTTCGGGACCAGCTCTTGGGAAAACC ATCTGAAGAGATTGTAGAGTGTCAGAAACCCAAACCAATCCTGCTTCACAGTGGAGAGCTGACGATACCA CATCCTTGGCAACCAGATATTGTTGATGTTCAGATTGTTACCGTTGGGTCCTTGGCCATAGCTGCTATCC CTGGGGAATTAACAACCATGTCGGGACGAAGATTTCGTGAGGCAATTAAAAAAGAATTTGCACTTTATGG GATGAAGGATATGACCGTTGTTATCGCAGGTCTAAGCAATGTTTATACACATTACATTACCACATATGAA GAATACCAGGCTCAGCGGTACGAGGCAGCATCTACAATCTATGGACCACACACCCTGTCTGCATACATCC AACTCTTCAGAGACCTTGCTAAGGCAATTGCTACGGACACAGTAGCCAACATGAGCAGTGGTCCCGAGCC TCCATTCTTCAAAAATCTAATAGCTTCACTTATTCCTAATATTGCGGATAGAGCACCAATTGGCAAACAT TTTGGGGATGTCTTGCAGCCAGCAAAACCTGAATACAGAGTGGGAGAAGTGGTTGAAGTTATATTTGTAG GCGCTAACCCAAAGAATTCAGCAGAGAACCAGACCCATCAAACCTTCCTCACTGTGGAGAAATACGAGGA CTCTGTAGCTGACTGGCAGATAATGTATAACGATGCCTCCTGGGAGACGAGGTTTTATTGGCACAAAGGA ATACTGGGTCTGAGCAATGCAACAATATACTGGCATATTCCAGATACTGCCTACCCTGGAATCTACAGAA TAAGATATTTTGGACACAATCGGAAGCAGGAACTTCTGAAACCCGCTGTCATACTAGCATTTGAAGGAAT TTCTTCTCCTTTTGAAGTTGTCACTACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224900 representing NM_018830
Red=Cloning site Green=Tags(s) MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFP SFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMIS QRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIA HTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHP VSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKS TCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHT VKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIP HPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYE EYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKH FGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKG ILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018830 |
| ORF Size | 2268 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018830.1, NP_061300.1 |
| RefSeq Size | 4835 bp |
| RefSeq ORF | 2271 bp |
| Locus ID | 54447 |
| UniProt ID | Q9JHE3 |
| MW | 84 kDa |
| Gene Summary | Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH (PubMed:10753931, PubMed:10652340, PubMed:16380386). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:14557071). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed:10652340, PubMed:21613224). Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells (PubMed:16126722). Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells (PubMed:16126722). This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract (PubMed:16380386).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221379 | Asah2 (untagged) - Mouse N-acylsphingosine amidohydrolase 2 (Asah2), (10ug) |
CNY 9,790.00 |
|
| MG224900 | Asah2 (tGFP-tagged) - Mouse N-acylsphingosine amidohydrolase 2 (Asah2), (10ug) |
CNY 8,270.00 |
|
| MR224900L3 | Lenti ORF clone of Asah2 (Myc-DDK-tagged) - Mouse N-acylsphingosine amidohydrolase 2 (Asah2) |
CNY 10,704.00 |
|
| MR224900L4 | Lenti ORF clone of Asah2 (mGFP-tagged) - Mouse N-acylsphingosine amidohydrolase 2 (Asah2) |
CNY 9,410.00 |
