Asah2 (NM_018830) Mouse Tagged ORF Clone

CAT#: MR224900

  • TrueORF®

Asah2 (Myc-DDK-tagged) - Mouse N-acylsphingosine amidohydrolase 2 (Asah2)

ORF Plasmid: DDK tGFP



  "NM_018830" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 8,304.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AI585898
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR224900 representing NM_018830
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAAGCGAACCTTCTCCACCTTGGAGGCATTCCTCATTTTCCTTCTGGTAATAATGACAGTCATCA
CAGTGGCCCTTCTCACCCTCTTGTTTGTTACCAGTGGGACCATTGAAAACCACAAAGATTCAGGAAATCA
CTGGTTTTCAACCACTCTGGGCTCCACGACAACCCAGCCCCCTCCAATTACACAGACTCCAAACTTCCCT
TCATTTCGGAACTTCAGTGGCTACTACATTGGCGTTGGGAGAGCAGATTGCACAGGACAAGTGTCAGATA
TCAATTTGATGGGCTATGGCAAAAATGGCCAGAATGCACGGGGTCTCCTCACCAGGCTGTTCAGCCGTGC
TTTTATCTTGGCGGATCCAGATGGGTCAAATCGAATGGCATTTGTGAGCGTGGAACTATGTATGATTTCC
CAACGACTGAGGTTGGAGGTCCTGAAGAGACTAGAGAGTAAATATGGCTCTCTGTATCGAAGAGACAATG
TTATCCTGAGTGCCATTCACACACACTCTGGCCCAGCAGGGTTTTTCCAATATACACTCTATATACTCGC
CAGCGAGGGATTCAGCAACCGGACCTTTCAGTACATAGTCTCTGGGATCATGAAGAGCATTGATATAGCT
CACACAAATCTTAAACCAGGCAAAATCTTTATCAACAAAGGAAATGTTGCTAATGTGCAGATCAACCGAA
GCCCCTCCTCTTACCTTCTGAATCCACAGTCAGAGAGAGCAAGGTATTCTTCAAACACAGACAAGGAAAT
GCTGGTCTTGAAACTGGTGGATTTGAATGGAGAAGACTTGGGTCTTATCAGCTGGTTTGCCATCCACCCC
GTGAGCATGAACAATAGCAACCACTTTGTGAATAGTGACAATATGGGCTATGCGGCTTACCTTTTTGAGC
AAGAAAAGAACAAAGGCTATCTGCCTGGACAGGGACCGTTTGTAGCAGGCTTTGCTTCATCAAATCTCGG
AGACGTGTCACCCAACATTCTTGGCCCGCATTGTGTCAACACAGGGGAGTCTTGTGACAACGACAAGAGC
ACCTGTCCCAACGGTGGGCCTAGCATGTGCATGGCCAGCGGACCTGGACAAGACATGTTTGAGAGCACAC
ACATTATAGGACGGATCATCTATCAGAAGGCCAAGGAGCTGTATGCCTCTGCCTCCCAGGAGGTGACCGG
CCCAGTGCTTGCAGCTCACCAGTGGGTGAACATGACAGATGTGAGCGTCCAGCTCAATGCCACACACACA
GTGAAGACGTGTAAACCTGCCCTGGGCTACAGTTTTGCCGCAGGCACAATTGATGGAGTTTCGGGCCTCA
ATATTACACAGGGAACTACGGAAGGGGATCCATTCTGGGACACTCTTCGGGACCAGCTCTTGGGAAAACC
ATCTGAAGAGATTGTAGAGTGTCAGAAACCCAAACCAATCCTGCTTCACAGTGGAGAGCTGACGATACCA
CATCCTTGGCAACCAGATATTGTTGATGTTCAGATTGTTACCGTTGGGTCCTTGGCCATAGCTGCTATCC
CTGGGGAATTAACAACCATGTCGGGACGAAGATTTCGTGAGGCAATTAAAAAAGAATTTGCACTTTATGG
GATGAAGGATATGACCGTTGTTATCGCAGGTCTAAGCAATGTTTATACACATTACATTACCACATATGAA
GAATACCAGGCTCAGCGGTACGAGGCAGCATCTACAATCTATGGACCACACACCCTGTCTGCATACATCC
AACTCTTCAGAGACCTTGCTAAGGCAATTGCTACGGACACAGTAGCCAACATGAGCAGTGGTCCCGAGCC
TCCATTCTTCAAAAATCTAATAGCTTCACTTATTCCTAATATTGCGGATAGAGCACCAATTGGCAAACAT
TTTGGGGATGTCTTGCAGCCAGCAAAACCTGAATACAGAGTGGGAGAAGTGGTTGAAGTTATATTTGTAG
GCGCTAACCCAAAGAATTCAGCAGAGAACCAGACCCATCAAACCTTCCTCACTGTGGAGAAATACGAGGA
CTCTGTAGCTGACTGGCAGATAATGTATAACGATGCCTCCTGGGAGACGAGGTTTTATTGGCACAAAGGA
ATACTGGGTCTGAGCAATGCAACAATATACTGGCATATTCCAGATACTGCCTACCCTGGAATCTACAGAA
TAAGATATTTTGGACACAATCGGAAGCAGGAACTTCTGAAACCCGCTGTCATACTAGCATTTGAAGGAAT
TTCTTCTCCTTTTGAAGTTGTCACTACT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR224900 representing NM_018830
Red=Cloning site Green=Tags(s)

MAKRTFSTLEAFLIFLLVIMTVITVALLTLLFVTSGTIENHKDSGNHWFSTTLGSTTTQPPPITQTPNFP
SFRNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILADPDGSNRMAFVSVELCMIS
QRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIA
HTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDLNGEDLGLISWFAIHP
VSMNNSNHFVNSDNMGYAAYLFEQEKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKS
TCPNGGPSMCMASGPGQDMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHT
VKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIP
HPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYGMKDMTVVIAGLSNVYTHYITTYE
EYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSSGPEPPFFKNLIASLIPNIADRAPIGKH
FGDVLQPAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYWHKG
ILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEVVTT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018830
ORF Size 2268 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018830.1, NP_061300.1
RefSeq Size 4835 bp
RefSeq ORF 2271 bp
Locus ID 54447
UniProt ID Q9JHE3
MW 84 kDa
Gene Summary Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH (PubMed:10753931, PubMed:10652340, PubMed:16380386). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:14557071). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed:10652340, PubMed:21613224). Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells (PubMed:16126722). Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells (PubMed:16126722). This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract (PubMed:16380386).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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