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Erap1 (NM_030711) Mouse Tagged ORF Clone
CAT＃: MR224333
- TrueORF®
Erap1 (Myc-DDK-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1)
ORF Plasmid: tGFP
"NM_030711" in other vectors (6)
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CNY 6,808.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Arts1; ERAAP; PILSA; PILSAP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224333 representing NM_030711
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCTCTTCTTCCCCTAGTATTGACATTTTTATCTGTGTCATCTCCTTCTTGGTGTCAGAACAGTG ATATAGAATCTCTAAAAGCTAGTAATGGAGACTCATTCCCTTGGAATAATATGCGACTTCCTGAGTATAT GACCCCGATTCATTATGATCTCATGATCCATGCAAACCTCAGCACTCTGACTTTCTGGGGAAAAACAGAA GTAGAAATCATAGCTAGCCGGCCCACCAGCACCATTATTATGCATAGTCACCACCTGCAAATATCTAAGG CCACCCTCAGAAGAGGAGCTGGAGAGATGCTGTCTGAAGAACCACTGAAGGTCCTGGAATATCCTGCTCA TGAGCAAGTTGCACTGCTGGCTGCCCAGCCGCTTCTTGCTGGGTCCCTGTACACAGTTATCATCGACTAT GCTGCCAACCTGTCTGAGAGTTTCCATGGATTCTATAAGAGCACCTACAGAACACAGGAAGGTGAAATGA GAATACTTGCAGCAACACAGTTTGAACCCACAGCTGCTAGAATGGCTTTTCCCTGCTTTGATGAACCTGC CCTCAAGGCAAGTTTTTCCATCAAGATAAAGAGGGATCCAAGGCACCTGGCCATCTCCAACATGCCCCTG GTGAAATCTGTGAATGTTGCTGAAGGACTCATAGAAGACCATTTTGACATCACTGTGAAGATGAGTACCT ACCTAGTGGCCTTCATCATTTCTGATTTTAAGTCTGTGAGCAAGATGACTAAGAGTGGAGTCAAGGTTTC TGTGTATGCTGTGCCAGACAAGATAAATCAAGCCGATTATGCTCTGGATGCTGCAGTGACTCTTCTAGAG TTTTATGAGGATTATTTCAACATTCCATATCCTTTACCCAAGCAAGATCTTGCTGCAATCCCAGACTTTC AGTCTGGTGCTATGGAAAACTGGGGACTGACCACATACAGAGAGTCCTCTCTGTTATACGATAAAGAAAA GTCTTCTGCATCTAGTAAGCTTGGCATCACAATGATTGTGTCCCATGAACTGGCTCACCAGTGGTTCGGG AACCTGGTCACCATGGAATGGTGGAATGACCTTTGGCTCAATGAAGGATTTGCCAAATTTATGGAGTTTG TGTCTGTCACTGTGACCCATCCTGAACTAAAAGTTGAAGACTATTTCTTTGGCAAGTGTTTTAATGCAAT GGAAGTTGATGCATTAAACTCCTCTCACCCTGTATCCACACCTGTGGAGAATCCTGCGCAGATTCGGGAG ATGTTTGATGATGTTTCTTATGAAAAGGGAGCTTGTATTCTGAATATGCTAAGAGATTATCTGAGTGCAG ATACATTTAAAAGAGGAATTGTACAGTATCTCCAAAAGTATAGTTATAAAAACACAAAAAACGAGGACCT GTGGAATAGCATGATGCATATTTGCCCTACAGATGGCACACAAACAATGGATGGCTTCTGCTCTAGAAGC CAACACTCATCATCAACCTCACACTGGCGTCAGGAGGTTGTAGATGTAAAGACCATGATGAACACATGGA CACTGCAGAAGGGCTTTCCTCTGATAACCATCACTGTGAGTGGGCGGAATGTGCACATGAAGCAAGAACA CTACATGAAGGGTTCAGAACGCTTCCCAGAGACTGGGTATTTGTGGCATGTTCCACTGACATTCATTACC AGCAAATCAGACTCAGTCCAGAGATTTTTGCTGAAGACAAAGACAGATGTGCTCATCCTCCCAGAAGCAG TGCAGTGGATCAAATTTAACGTGGGAATGAATGGCTATTACATTGTGCATTACGCGGATGATGGATGGGC TTCTCTGAGTGGCCTTTTAAAAGAAGCGCACACAACAATCAGCAGTAATGATCGGGCAAGTCTCATCAAC AATGCATTTCAGCTGGTCAGCATTGAAAAGTTATCAATAGAAAAAGCCCTGGATTTAACCCTGTACTTGA AAAATGAAACTGAAATTATGCCCATATTCCAAGCTTTGAATGAACTCATACCTATGTATAAGTTAATGGA GAAAAGAGATATGATTGAGGTGGAAACTCAATTTAAGGACTTCCTTCTCAAGTTGCTGAAGGACCTTATT GATAAGCAGACTTGGACAGATGAAGGCTCTGTCTCAGAGAGGATGCTCAGGAGCCAGCTCCTCCTCCTCG CATGTGTGCGCAATTATCAGCCCTGTGTGCAGAGGGCAGAGCGCTATTTCAGAGAGTGGAAGTCATCTAA TGGAAACATGAGCATCCCTATTGATGTGACCTTGGCTGTGTTTGCTGTCGGGGCCCAAAACACAGAAGGC TGGGATTTTCTTTATAGTAAATATCAGTCTTCTTTGTCTAGTACTGAGAAAAGCCAAATTGAATTTTCCC TCTGTACAAGCAAAGATCCAGAAAAACTTCAGTGGCTTCTAGATCAAAGTTTTAAGGGAGAGATAATAAA AACTCAGGAATTTCCACATATTCTCACACTCATTGGCAGAAACCCAGTGGGTTATCCATTGGCCTGGAAG TTTCTGAGGGAAAATTGGAATAAGCTTGTACAGAAATTTGAACTTGGCTCATCGTCCATAGCTCACATGG TAATGGGAACAACAGACCAGTTTTCCACCAGAGCACGTCTTGAAGAGGTAAAAGGATTCTTCAGTTCCTT GAAAGAAAACGGTTCTCAGCTCCGTTGTGTTCAACAAACCATTGAGACCATTGAAGAAAACATACGATGG ATGGATAAGAATTTTGATAAAATAAGACTGTGGCTGCAAAAAGAAAAGCCAGAGTTGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224333 representing NM_030711
Red=Cloning site Green=Tags(s) MPSLLPLVLTFLSVSSPSWCQNSDIESLKASNGDSFPWNNMRLPEYMTPIHYDLMIHANLSTLTFWGKTE VEIIASRPTSTIIMHSHHLQISKATLRRGAGEMLSEEPLKVLEYPAHEQVALLAAQPLLAGSLYTVIIDY AANLSESFHGFYKSTYRTQEGEMRILAATQFEPTAARMAFPCFDEPALKASFSIKIKRDPRHLAISNMPL VKSVNVAEGLIEDHFDITVKMSTYLVAFIISDFKSVSKMTKSGVKVSVYAVPDKINQADYALDAAVTLLE FYEDYFNIPYPLPKQDLAAIPDFQSGAMENWGLTTYRESSLLYDKEKSSASSKLGITMIVSHELAHQWFG NLVTMEWWNDLWLNEGFAKFMEFVSVTVTHPELKVEDYFFGKCFNAMEVDALNSSHPVSTPVENPAQIRE MFDDVSYEKGACILNMLRDYLSADTFKRGIVQYLQKYSYKNTKNEDLWNSMMHICPTDGTQTMDGFCSRS QHSSSTSHWRQEVVDVKTMMNTWTLQKGFPLITITVSGRNVHMKQEHYMKGSERFPETGYLWHVPLTFIT SKSDSVQRFLLKTKTDVLILPEAVQWIKFNVGMNGYYIVHYADDGWASLSGLLKEAHTTISSNDRASLIN NAFQLVSIEKLSIEKALDLTLYLKNETEIMPIFQALNELIPMYKLMEKRDMIEVETQFKDFLLKLLKDLI DKQTWTDEGSVSERMLRSQLLLLACVRNYQPCVQRAERYFREWKSSNGNMSIPIDVTLAVFAVGAQNTEG WDFLYSKYQSSLSSTEKSQIEFSLCTSKDPEKLQWLLDQSFKGEIIKTQEFPHILTLIGRNPVGYPLAWK FLRENWNKLVQKFELGSSSIAHMVMGTTDQFSTRARLEEVKGFFSSLKENGSQLRCVQQTIETIEENIRW MDKNFDKIRLWLQKEKPELL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030711 |
| ORF Size | 2790 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030711.5 |
| RefSeq Size | 4014 bp |
| RefSeq ORF | 2793 bp |
| Locus ID | 80898 |
| UniProt ID | Q9EQH2 |
| MW | 107 kDa |
| Gene Summary | Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222694 | Erap1 (untagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1), (10ug) |
CNY 11,970.00 |
|
| MG224333 | Erap1 (tGFP-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1), (10ug) |
CNY 7,890.00 |
|
| MR224333L1 | Lenti ORF clone of Erap1 (Myc-DDK-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1) |
CNY 9,030.00 |
|
| MR224333L2 | Lenti ORF clone of Erap1 (mGFP-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1) |
CNY 9,208.00 |
|
| MR224333L3 | Lenti ORF clone of Erap1 (Myc-DDK-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1) |
CNY 9,030.00 |
|
| MR224333L4 | Lenti ORF clone of Erap1 (mGFP-tagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1) |
CNY 9,030.00 |
