Erap1 (NM_030711) Mouse Untagged Clone

CAT#: MC222694

Erap1 (untagged) - Mouse endoplasmic reticulum aminopeptidase 1 (Erap1), (10ug)



  "NM_030711" in other vectors (6)

CNY 11,970.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Arts1; ERAAP; PILSA; PILSAP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222694 representing NM_030711
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTCTCTTCTTCCCCTAGTATTGACATTTTTATCTGTGTCATCTCCTTCTTGGTGTCAGAACAGTG
ATATAGAATCTCTAAAAGCTAGTAATGGAGACTCATTCCCTTGGAATAATATGCGACTTCCTGAGTATAT
GACCCCGATTCATTATGATCTCATGATCCATGCAAACCTCAGCACTCTGACTTTCTGGGGAAAAACAGAA
GTAGAAATCATAGCTAGCCGGCCCACCAGCACCATTATTATGCATAGTCACCACCTGCAAATATCTAAGG
CCACCCTCAGAAGAGGAGCTGGAGAGATGCTGTCTGAAGAACCACTGAAGGTCCTGGAATATCCTGCTCA
TGAGCAAGTTGCACTGCTGGCTGCCCAGCCGCTTCTTGCTGGGTCCCTGTACACAGTTATCATCGACTAT
GCTGCCAACCTGTCTGAGAGTTTCCATGGATTCTATAAGAGCACCTACAGAACACAGGAAGGTGAAATGA
GAATACTTGCAGCAACACAGTTTGAACCCACAGCTGCTAGAATGGCTTTTCCCTGCTTTGATGAACCTGC
CCTCAAGGCAAGTTTTTCCATCAAGATAAAGAGGGATCCAAGGCACCTGGCCATCTCCAACATGCCCCTG
GTGAAATCTGTGAATGTTGCTGAAGGACTCATAGAAGACCATTTTGACATCACTGTGAAGATGAGTACCT
ACCTAGTGGCCTTCATCATTTCTGATTTTAAGTCTGTGAGCAAGATGACTAAGAGTGGAGTCAAGGTTTC
TGTGTATGCTGTGCCAGACAAGATAAATCAAGCCGATTATGCTCTGGATGCTGCAGTGACTCTTCTAGAG
TTTTATGAGGATTATTTCAACATTCCATATCCTTTACCCAAGCAAGATCTTGCTGCAATCCCAGACTTTC
AGTCTGGTGCTATGGAAAACTGGGGACTGACCACATACAGAGAGTCCTCTCTGTTATACGATAAAGAAAA
GTCTTCTGCATCTAGTAAGCTTGGCATCACAATGATTGTGTCCCATGAACTGGCTCACCAGTGGTTCGGG
AACCTGGTCACCATGGAATGGTGGAATGACCTTTGGCTCAATGAAGGATTTGCCAAATTTATGGAGTTTG
TGTCTGTCACTGTGACCCATCCTGAACTAAAAGTTGAAGACTATTTCTTTGGCAAGTGTTTTAATGCAAT
GGAAGTTGATGCATTAAACTCCTCTCACCCTGTATCCACACCTGTGGAGAATCCTGCGCAGATTCGGGAG
ATGTTTGATGATGTTTCTTATGAAAAGGGAGCTTGTATTCTGAATATGCTAAGAGATTATCTGAGTGCAG
ATACATTTAAAAGAGGAATTGTACAGTATCTCCAAAAGTATAGTTATAAAAACACAAAAAACGAGGACCT
GTGGAATAGCATGATGCATATTTGCCCTACAGATGGCACACAAACAATGGATGGCTTCTGCTCTAGAAGC
CAACACTCATCATCAACCTCACACTGGCGTCAGGAGGTTGTAGATGTAAAGACCATGATGAACACATGGA
CACTGCAGAAGGGCTTTCCTCTGATAACCATCACTGTGAGTGGGCGGAATGTGCACATGAAGCAAGAACA
CTACATGAAGGGTTCAGAACGCTTCCCAGAGACTGGGTATTTGTGGCATGTTCCACTGACATTCATTACC
AGCAAATCAGACTCAGTCCAGAGATTTTTGCTGAAGACAAAGACAGATGTGCTCATCCTCCCAGAAGCAG
TGCAGTGGATCAAATTTAACGTGGGAATGAATGGCTATTACATTGTGCATTACGCGGATGATGGATGGGC
TTCTCTGAGTGGCCTTTTAAAAGAAGCGCACACAACAATCAGCAGTAATGATCGGGCAAGTCTCATCAAC
AATGCATTTCAGCTGGTCAGCATTGAAAAGTTATCAATAGAAAAAGCCCTGGATTTAACCCTGTACTTGA
AAAATGAAACTGAAATTATGCCCATATTCCAAGCTTTGAATGAACTCATACCTATGTATAAGTTAATGGA
GAAAAGAGATATGATTGAGGTGGAAACTCAATTTAAGGACTTCCTTCTCAAGTTGCTGAAGGACCTTATT
GATAAGCAGACTTGGACAGATGAAGGCTCTGTCTCAGAGAGGATGCTCAGGAGCCAGCTCCTCCTCCTCG
CATGTGTGCGCAATTATCAGCCCTGTGTGCAGAGGGCAGAGCGCTATTTCAGAGAGTGGAAGTCATCTAA
TGGAAACATGAGCATCCCTATTGATGTGACCTTGGCTGTGTTTGCTGTCGGGGCCCAAAACACAGAAGGC
TGGGATTTTCTTTATAGTAAATATCAGTCTTCTTTGTCTAGTACTGAGAAAAGCCAAATTGAATTTTCCC
TCTGTACAAGCAAAGATCCAGAAAAACTTCAGTGGCTTCTAGATCAAAGTTTTAAGGGAGAGATAATAAA
AACTCAGGAATTTCCACATATTCTCACACTCATTGGCAGAAACCCAGTGGGTTATCCATTGGCCTGGAAG
TTTCTGAGGGAAAATTGGAATAAGCTTGTACAGAAATTTGAACTTGGCTCATCGTCCATAGCTCACATGG
TAATGGGAACAACAGACCAGTTTTCCACCAGAGCACGTCTTGAAGAGGTAAAAGGATTCTTCAGTTCCTT
GAAAGAAAACGGTTCTCAGCTCCGTTGTGTTCAACAAACCATTGAGACCATTGAAGAAAACATACGATGG
ATGGATAAGAATTTTGATAAAATAAGACTGTGGCTGCAAAAAGAAAAGCCAGAGTTGCTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_030711
Insert Size 2793 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_030711.4, NP_109636.1
RefSeq Size 4014 bp
RefSeq ORF 2793 bp
Locus ID 80898
UniProt ID Q9EQH2
Gene Summary Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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