Dennd4c (NM_184088) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1700065A05Rik; AA420392
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222752 representing NM_184088 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAGAAGACAAAGGACCTAGAGTGACAGACTACTTCGTTGTGGCAGGTCTCACTGATACATCCACTC TTTTGGATCAAGAAATAAATCGTACAGATACTAACTCGATTGGTCCTAAAGCTCCAATCACAGACATTGC TGTTATTATCAAATCAGCTGGGGAAACTGTACCTGAAGGCTACACCTGTGTGGAAGCCACTCCATCAGCA CTCCAAGCAAACTTGAATTATGGAAGTCTGAAGAGCCCAGAGCTTTTCCTGTGCTACAGGAGAGGGAGAG ATAAGCCGCCCCTTACAGATATTGGCGTGCTATATGAAGGGAAAGAAAGGCTTATGCCAGGATGTGAAGT GATCCAAGCCACGCCGTACGGCCGCTGTGCCAATGTCAACAATAGTTCAACTACTTCTCAAAGAATCTTT ATCACCTATCGAAGGGCTCCCCCAGTGCGGTCCCAGAATTCCTTGGCCGTCACTGATATCTGTGTTATTA TAACCAGTAAAGGAGAAACCCCTCCGCATACTTTCTGCAAAGTGGATAAAAATTTAAACTGTGGCATGTG GGGTTCCAACGTGTTTCTGTGTTACAAGAAGTCTGTGCCTGCTTCAAATGCAATAGCATATAAGGCTGGT TTGATTTTTCGATATCCGGAAGAAGACTATGAGTCTTTTCCGCTCTCACCATCTGTGCCTCTCTTCTGCC TTCCTATGGGAGCCACTATTGAATGCTGGGATCCTCAAATCAAATACCCACTTCCTGTTTTCTCAACATT TGTCCTGACAGGGTCTTCAGCTGAAAAGGTATATGGAGCTGCCATCCAGTTTTATGAACCTTACTCTCAA GAACGTCTAACAGAGAAACAACTTACACAGCTGGGCCTATTGACCCTCGTGGAGAAAAGAGTGGTCTCCA AACCCATCAATTCAAACAAGTGCATTTGTTTGCTTTCACACTGGCCTTTTTTTGAAGCTTTTAAGAATTT TCTTATGTTTATCTACAAAGTTTCTGTGTCTGGACCACATCCTCTTCCCATTGAAAAGCACATTTCACAT TTTATGCAAAATATCCCTTTTCCTTCACCACAAAGACCAAGAATCCTCATCCAGCTATCAGTCCATGATG CATTTATATTATCACAGCCAGTGTCCACACCTTTACCATTAAGTGGCGCCAACTTTAGCTCCTTGCTGAT GAATCTGGGTCCTGAGAACTGCGCGACATTGCTGCTGCTGGTTTTGCTCGAGAGTAAGATTCTGCTGCAC TCTCTGAGGCCAGCGGTACTAACTGGGGTAGCCGAAGCTGTCGTTGCTATGATCTTTCCATTTCAGTGGC AGTGCCCATATATCCCCCTCTGTCCTCTTTCATTGGCTGGAGTGCTTAGTGCGCCTTTACCATTTATAGT TGGAGTGGACTCAAGGTACTTTGATCTTCATGACCCACCACAAGATGTTGTCTGCATTGACTTGGATACG AACACATTATATGTAGCTGATGAAAGAAAGAACATAAACTGGAAGCAGCTTCCCAAAAGGCCGTGCAAGA GTCTGCTTGGCACCCTGAGGAGGCTGTATCAGCAGCTCTGCTCAGTTCACCGAAAACCTCAAGAAAGTTC AGCAATTGAGATGACTCCAATTGAAGCAGATTACTCTTGGCAAAAAAAGATGACACAATTGGAGATGGAA ATTCAGGAGACATTTTTGCGATTTATGGCGTCTATTTTAAAAGGGTACAGGTCATATCTCAGACCAATCA CAGAAGCTCCTTCTAATAAAGCCACAGCTGCGGATTCATTGTTTGATCGACAGGGTTTTTTGAAAAGTCG GGATCGTGCCTATACAAAATTCTATACTCTTTTATCCAAAACACAGATTTTTATTCGTTTCATTGAAGAA TGCAGTTTTGTAAGTGATAAAGATACTGGATTGGCATTTTTTGATGATTGCATCGAGAAGTTGTTTCCAG ATAAAGGTGTAGAGAGAACAGAAAAGGTTGACTTGGATTCAGCAGAAGACACCAGATTGATAGAGCTAGA TGATTCACAGAGAAGTGAACACACTGTATTTATAATGCCACCTGAGCCGCCTCCTGATGATGGAAATAAC TTATCACCTCAGTACAGTTACACATACTTTCCAAGATTGGATCTTAAGCTTTTTGACAGCCCACAGAAGC TGAAACTGTGCTTCAATAGACATCCTCCTGGGAGTAGCATTACAAACAGCCCTGCCCTCATGGCTAAAAG GACTAAACAGGAGATAAAAACAGCTCATAAGTTGGCGAAGAGATGTTATACGAATCCACCACAATGGGCC AAGTATCTATTCAGCCACTGTTACAGTCTGTGGTTTATTTGTCTCCCAGCCTATGTTAGAGTTTCTCACC CTAAGGTCCGAGCACTCCAGCAGGCACATGATGTGCTTGTTAAGATGCGGAAGACAGACGTGGATCCACT AGATGAGGTGTGCTATCGAGTAGTCATGCAGCTTTGTGGACTTTGGGTTAATCCTGTTTTAGCAGTGAGA GTCTTATTCGAGATGAAAACTGCTAGAATAAAACCAAATGCTATCACTTATGGCTATTACAATAAGGTAG TTTTGGAGAGCCCATGGCCCAGCAGTACTCGCAGTGGTATTTTCTTGTGGACGAAAGTACGGAATGTTGT ACATGGTTTGGCCCAGTTTAGGCAACCACTTAAAAAGACTGGGCAAAAATCACAGGTCTTTTCAATATCA GGCGGTCAGTCTGACCAGGGCTACGGGTCTAAGGATGAGCTGGTAAAGGAGGGTGCAGATGGTCACGCCC CTGAAGAGCACACGCCACCAGAGCTGACGACCACAGAGCTGCACATCGAAGAGGAGTGTGATATATCTGC CATTGTCTCAAAACACCTGCAGCCTACTCCTGAACCTCAGAGTCCTACGGAGCCTCCTGCATGGGGCAGC AGTATTGTGAAAGTTCCATCTGGGTTATTTGATACCAACAATAGGACAAGTACTGGTAGTACTTCAACTG TGCTATTTTCTACTCAAGCTCCAGTCGAGGATGCAGTCTTCAGTGAGGTTACTAATTTCAAGAAGAATGG TGATAGAGGAGAGAAAAAACAGAAGCATTTCCCTGAGAGGAGCTGTAGCTTTAGTTCTGAAAGTCGAGCG GGGATGCTGCTTAAGAAGAGCAGTTTGGACCTGAATTCAAGCGAAATGGCCATCATGATGGGAGCAGATG CCAAGATCCTCACAGCAGCACTGACATGTCCTAAGACTTCCCCGCCTCATGTCACAAGAACTCACAGCTT TGAGAATGTTAACTGTCATCTAGCTGACAGTAGGACTCGCATGTCAGAAGGCACCCGGGATTCTGAGCAC AGATCATCTCCTGTGCTAGAGATGCTGGAGGAAAGCCAGGAGCTCCTGGAGCCTGTGGTTGGTGATAATG TGGCAGAGACTGCGGCAGAGATGACGTGTAACAGTCTACAAAGTAACAGTCACAGTGACCAGTCCAGAGA CACACAAGCTGGAGCCCAAGATCCAGTGAACAAGAGGAGCAGTTCATATGCTACTAGGAAAGCCATTGAG AGGGAGGATGTGGAGACGGGACTAGATCCGTTGTCTCTTTTAGCCACTGAATGTGTAGAAAAAACGTCTG ATTCTGAAGATAAGTTATTTTCTCCAGTAATTTCACGTAATCTGGCTGATGAAATTGAAAGTTACATGAA CCTAAAAAGCCCACTAGGCAGTAAATCTTGTAGTATGGAATTGCATGGAGAGGGGAACCAGGAGCCTGGC TCTCCTGCTGTGTTTGCTCACCCTTTAGAAAGGAGCTCGAGTCTGCCTTCAGACCGTGGCCCGCCAGCAC GAGACAGCACCGAGACTGAGAAGAGCTCCCCTGCTGTGTCCAGCTCTAAAACTCTGACTGGGCGTTTCAA GCCACAGAGTCCTTACCGCGCTTACAAAGACCGGTCCACTTCCTTATCAGCATTAGTACGTTCTTCACCA AACAGCTCTCTGGGTTCTGTAGTCAATTCTCTATCGGGGCTAAAGTTGGACAATATACTCTCAGGGCCCA AGATAGATGTCCTAAAATCTAGTATGAAACAAGCAGCAACGGTGGCCAGTAAGATGTGGGTGGCTGTAGC ATCTGCCTATAGCTACTCAGATGACGAGGAGGAAACTAATAAAGATTACAGTTTCCCAGCTGGCCTAGAA GACCATCATATAGTGGGAGAGACTCTGTCGCCTAACACAAGTGTCTCAGGGTTGGTTCCCAGTGAACTGA CCCAGAGCAACACAAGCCTCGGCAGTAGTAGCAGCAGTGGAGACGTTGGAAAACTGCAGTGTCCAGCAGG TGAAGTTCCATTTTCAAGAAATATCAAAGGGCAGGACTTTGAAAAGTCAGACCATGGTTCTTCTCAGAAT ACCAGTATGTCTAGCATCTATCAAAATTGTGCGATGGAGGTTTTGATGTCGAGTTGCTCACAGTGCAGAG CTTGTGGCGCTCTGGTTTATGATGAGGAAATCATGGCTGGATGGACAGCAGATGACTCAAATTTGAATAC AACCTGCCCGTTCTGTAAAAGCAACTTCTTGCCTCTTCTCAATGTAGAATTTAAAGACCTGCGAGGCTCT GCCAGCTTTTTCTTGAAACCAAGCACGTCTGGTGACAGTTTACAAAGCGGCAGCATTCCATCAGCCAGTG AGCCCTCTGAACACAAACCCACGTCCAGTTCAGCAGAACCTGACCTGATCAGCTTTATGGATTTCTCAAA ACACAGTGAGACCATAACAGAAGAAGCAAGTTATACAGTCGAATCAAGTGATGAAATAAAGAAAACCAAT GGTGATGTCCAGAGTGTGAAAATGTCATCTGTGCCTAACAGTTTATCTAAGCGAAACGTATCTTTGACTC GAAGTCACAGTGTTGGAGGCCCTCTGCAGAATATCGACTTCTCCCAGCGACCATTTCATGGCGTCTCAAC AGTCAGTCTTCCAAGCAGTCTGCAGGAAGATGTGGACCATCTAGGCAAGCGACCCAGTCCTCCTCCTGTG TCTGTGCCCTATTTGAGTCCTCTAGTGCTTCGGAAGGAACTTGAATCTTTGCTAGAAAATGAAGGTGATC AGGTCATCCATACATCCTCATTCATCAATCAACACCCAATCATTTTCTGGAACCTGGTTTGGTATTTCAG ACGTTTGGACCTTCCTAGCAACTTGCCAGGCCTTATTCTCACCTCTGAGCATTGTAATGGAGGTGTGCAG CTTCCTCTATCATCTTTGTCCCAGGACAGCAAACTGGTGTATATTCAACTGCTGTGGGATAATATCAACC TTCATCAGGAACCAGGAGAACCTCTGTATGTCTCTTGGAGGAACTTGAATTCTGAGAAGAAGCCGTCACT TCTGTCAGAGCAGCAGCAGGCAGCCAGCGCTTTAGTGGAGACGATCAGGCAGAGCATCCAGCAGAATGAC GTTCTCAAGCCCATCAACCTGCTTTCCCAGCAAATGAAGCCAGGCACGAAACGGCAGAGGAGTTTATACA GAGAAATCCTTTTCTTATCATTAGTGTCTCTTGGGAGAGAAAACATTGATATTGAGGCTTTTGACAATGA ATATGGACTCGCATACAGAAGTCTGCCTTCAGAGAGTCTTGAAAGGTTACAGAGAATCGATGCCCCACCC AGTATCAGTGTGGAGTGGTGCAGGAAGTGTTTTGGAGCGCCTCTCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222752 representing NM_184088 Red=Cloning site Green=Tags(s) MIEDKGPRVTDYFVVAGLTDTSTLLDQEINRTDTNSIGPKAPITDIAVIIKSAGETVPEGYTCVEATPSA LQANLNYGSLKSPELFLCYRRGRDKPPLTDIGVLYEGKERLMPGCEVIQATPYGRCANVNNSSTTSQRIF ITYRRAPPVRSQNSLAVTDICVIITSKGETPPHTFCKVDKNLNCGMWGSNVFLCYKKSVPASNAIAYKAG LIFRYPEEDYESFPLSPSVPLFCLPMGATIECWDPQIKYPLPVFSTFVLTGSSAEKVYGAAIQFYEPYSQ ERLTEKQLTQLGLLTLVEKRVVSKPINSNKCICLLSHWPFFEAFKNFLMFIYKVSVSGPHPLPIEKHISH FMQNIPFPSPQRPRILIQLSVHDAFILSQPVSTPLPLSGANFSSLLMNLGPENCATLLLLVLLESKILLH SLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLAGVLSAPLPFIVGVDSRYFDLHDPPQDVVCIDLDT NTLYVADERKNINWKQLPKRPCKSLLGTLRRLYQQLCSVHRKPQESSAIEMTPIEADYSWQKKMTQLEME IQETFLRFMASILKGYRSYLRPITEAPSNKATAADSLFDRQGFLKSRDRAYTKFYTLLSKTQIFIRFIEE CSFVSDKDTGLAFFDDCIEKLFPDKGVERTEKVDLDSAEDTRLIELDDSQRSEHTVFIMPPEPPPDDGNN LSPQYSYTYFPRLDLKLFDSPQKLKLCFNRHPPGSSITNSPALMAKRTKQEIKTAHKLAKRCYTNPPQWA KYLFSHCYSLWFICLPAYVRVSHPKVRALQQAHDVLVKMRKTDVDPLDEVCYRVVMQLCGLWVNPVLAVR VLFEMKTARIKPNAITYGYYNKVVLESPWPSSTRSGIFLWTKVRNVVHGLAQFRQPLKKTGQKSQVFSIS GGQSDQGYGSKDELVKEGADGHAPEEHTPPELTTTELHIEEECDISAIVSKHLQPTPEPQSPTEPPAWGS SIVKVPSGLFDTNNRTSTGSTSTVLFSTQAPVEDAVFSEVTNFKKNGDRGEKKQKHFPERSCSFSSESRA GMLLKKSSLDLNSSEMAIMMGADAKILTAALTCPKTSPPHVTRTHSFENVNCHLADSRTRMSEGTRDSEH RSSPVLEMLEESQELLEPVVGDNVAETAAEMTCNSLQSNSHSDQSRDTQAGAQDPVNKRSSSYATRKAIE REDVETGLDPLSLLATECVEKTSDSEDKLFSPVISRNLADEIESYMNLKSPLGSKSCSMELHGEGNQEPG SPAVFAHPLERSSSLPSDRGPPARDSTETEKSSPAVSSSKTLTGRFKPQSPYRAYKDRSTSLSALVRSSP NSSLGSVVNSLSGLKLDNILSGPKIDVLKSSMKQAATVASKMWVAVASAYSYSDDEEETNKDYSFPAGLE DHHIVGETLSPNTSVSGLVPSELTQSNTSLGSSSSSGDVGKLQCPAGEVPFSRNIKGQDFEKSDHGSSQN TSMSSIYQNCAMEVLMSSCSQCRACGALVYDEEIMAGWTADDSNLNTTCPFCKSNFLPLLNVEFKDLRGS ASFFLKPSTSGDSLQSGSIPSASEPSEHKPTSSSAEPDLISFMDFSKHSETITEEASYTVESSDEIKKTN GDVQSVKMSSVPNSLSKRNVSLTRSHSVGGPLQNIDFSQRPFHGVSTVSLPSSLQEDVDHLGKRPSPPPV SVPYLSPLVLRKELESLLENEGDQVIHTSSFINQHPIIFWNLVWYFRRLDLPSNLPGLILTSEHCNGGVQ LPLSSLSQDSKLVYIQLLWDNINLHQEPGEPLYVSWRNLNSEKKPSLLSEQQQAASALVETIRQSIQQND VLKPINLLSQQMKPGTKRQRSLYREILFLSLVSLGRENIDIEAFDNEYGLAYRSLPSESLERLQRIDAPP SISVEWCRKCFGAPLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_184088
ORF Size	5718 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_184088.1, NP_908976.1
RefSeq Size	7793 bp
RefSeq ORF	5721 bp
Locus ID	329877
UniProt ID	A6H8H2
MW	211.5 kDa
Gene Summary	Guanine nucleotide exchange factor (GEF) activating RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB10 into its active GTP-bound form. Thereby, stimulates SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane in response to insulin.[UniProtKB/Swiss-Prot Function]

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