Dennd4c (NM_184088) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700065A05Rik; AA420392
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225031 representing NM_184088 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAGAAGACAAAGGACCTAGAGTGACAGACTACTTCGTTGTGGCAGGTCTCACTGATACATCCACTC TTTTGGATCAAGAAATAAATCGTACAGATACTAACTCGATTGGTCCTAAAGCTCCAATCACAGACATTGC TGTTATTATCAAATCAGCTGGGGAAACTGTACCTGAAGGCTACACCTGTGTGGAAGCCACTCCATCAGCA CTCCAAGCAAACTTGAATTATGGAAGTCTGAAGAGCCCAGAGCTTTTCCTGTGCTACAGGAGAGGGAGAG ATAAGCCGCCCCTTACAGATATTGGCGTGCTATATGAAGGGAAAGAAAGGCTTATGCCAGGATGTGAAGT GATCCAAGCCACGCCGTACGGCCGCTGTGCCAATGTCAACAATAGTTCAACTACTTCTCAAAGAATCTTT ATCACCTATCGAAGGGCTCCCCCAGTGCGGTCCCAGAATTCCTTGGCCGTCACTGATATCTGTGTTATTA TAACCAGTAAAGGAGAAACCCCTCCGCATACTTTCTGCAAAGTGGATAAAAATTTAAACTGTGGCATGTG GGGTTCCAACGTGTTTCTGTGTTACAAGAAGTCTGTGCCTGCTTCAAATGCAATAGCATATAAGGCTGGT TTGATTTTTCGATATCCGGAAGAAGACTATGAGTCTTTTCCGCTCTCACCATCTGTGCCTCTCTTCTGCC TTCCTATGGGAGCCACTATTGAATGCTGGGATCCTCAAATCAAATACCCACTTCCTGTTTTCTCAACATT TGTCCTGACAGGGTCTTCAGCTGAAAAGGTATATGGAGCTGCCATCCAGTTTTATGAACCTTACTCTCAA GAACGTCTAACAGAGAAACAACTTACACAGCTGGGCCTATTGACCCTCGTGGAGAAAAGAGTGGTCTCCA AACCCATCAATTCAAACAAGTGCATTTGTTTGCTTTCACACTGGCCTTTTTTTGAAGCTTTTAAGAATTT TCTTATGTTTATCTACAAAGTTTCTGTGTCTGGACCACATCCTCTTCCCATTGAAAAGCACATTTCACAT TTTATGCAAAATATCCCTTTTCCTTCACCACAAAGACCAAGAATCCTCATCCAGCTATCAGTCCATGATG CATTTATATTATCACAGCCAGTGTCCACACCTTTACCATTAAGTGGCGCCAACTTTAGCTCCTTGCTGAT GAATCTGGGTCCTGAGAACTGCGCGACATTGCTGCTGCTGGTTTTGCTCGAGAGTAAGATTCTGCTGCAC TCTCTGAGGCCAGCGGTACTAACTGGGGTAGCCGAAGCTGTCGTTGCTATGATCTTTCCATTTCAGTGGC AGTGCCCATATATCCCCCTCTGTCCTCTTTCATTGGCTGGAGTGCTTAGTGCGCCTTTACCATTTATAGT TGGAGTGGACTCAAGGTACTTTGATCTTCATGACCCACCACAAGATGTTGTCTGCATTGACTTGGATACG AACACATTATATGTAGCTGATGAAAGAAAGAACATAAACTGGAAGCAGCTTCCCAAAAGGCCGTGCAAGA GTCTGCTTGGCACCCTGAGGAGGCTGTATCAGCAGCTCTGCTCAGTTCACCGAAAACCTCAAGAAAGTTC AGCAATTGAGATGACTCCAATTGAAGCAGATTACTCTTGGCAAAAAAAGATGACACAATTGGAGATGGAA ATTCAGGAGACATTTTTGCGATTTATGGCGTCTATTTTAAAAGGGTACAGGTCATATCTCAGACCAATCA CAGAAGCTCCTTCTAATAAAGCCACAGCTGCGGATTCATTGTTTGATCGACAGGGTTTTTTGAAAAGTCG GGATCGTGCCTATACAAAATTCTATACTCTTTTATCCAAAACACAGATTTTTATTCGTTTCATTGAAGAA TGCAGTTTTGTAAGTGATAAAGATACTGGATTGGCATTTTTTGATGATTGCATCGAGAAGTTGTTTCCAG ATAAAGGTGTAGAGAGAACAGAAAAGGTTGACTTGGATTCAGCAGAAGACACCAGATTGATAGAGCTAGA TGATTCACAGAGAAGTGAACACACTGTATTTATAATGCCACCTGAGCCGCCTCCTGATGATGGAAATAAC TTATCACCTCAGTACAGTTACACATACTTTCCAAGATTGGATCTTAAGCTTTTTGACAGCCCACAGAAGC TGAAACTGTGCTTCAATAGACATCCTCCTGGGAGTAGCATTACAAACAGCCCTGCCCTCATGGCTAAAAG GACTAAACAGGAGATAAAAACAGCTCATAAGTTGGCGAAGAGATGTTATACGAATCCACCACAATGGGCC AAGTATCTATTCAGCCACTGTTACAGTCTGTGGTTTATTTGTCTCCCAGCCTATGTTAGAGTTTCTCACC CTAAGGTCCGAGCACTCCAGCAGGCACATGATGTGCTTGTTAAGATGCGGAAGACAGACGTGGATCCACT AGATGAGGTGTGCTATCGAGTAGTCATGCAGCTTTGTGGACTTTGGGTTAATCCTGTTTTAGCAGTGAGA GTCTTATTCGAGATGAAAACTGCTAGAATAAAACCAAATGCTATCACTTATGGCTATTACAATAAGGTAG TTTTGGAGAGCCCATGGCCCAGCAGTACTCGCAGTGGTATTTTCTTGTGGACGAAAGTACGGAATGTTGT ACATGGTTTGGCCCAGTTTAGGCAACCACTTAAAAAGACTGGGCAAAAATCACAGGTCTTTTCAATATCA GGCGGTCAGTCTGACCAGGGCTACGGGTCTAAGGATGAGCTGGTAAAGGAGGGTGCAGATGGTCACGCCC CTGAAGAGCACACGCCACCAGAGCTGACGACCACAGAGCTGCACATCGAAGAGGAGTGTGATATATCTGC CATTGTCTCAAAACACCTGCAGCCTACTCCTGAACCTCAGAGTCCTACGGAGCCTCCTGCATGGGGCAGC AGTATTGTGAAAGTTCCATCTGGGTTATTTGATACCAACAATAGGACAAGTACTGGTAGTACTTCAACTG TGCTATTTTCTACTCAAGCTCCAGTCGAGGATGCAGTCTTCAGTGAGGTTACTAATTTCAAGAAGAATGG TGATAGAGGAGAGAAAAAACAGAAGCATTTCCCTGAGAGGAGCTGTAGCTTTAGTTCTGAAAGTCGAGCG GGGATGCTGCTTAAGAAGAGCAGTTTGGACCTGAATTCAAGCGAAATGGCCATCATGATGGGAGCAGATG CCAAGATCCTCACAGCAGCACTGACATGTCCTAAGACTTCCCCGCCTCATGTCACAAGAACTCACAGCTT TGAGAATGTTAACTGTCATCTAGCTGACAGTAGGACTCGCATGTCAGAAGGCACCCGGGATTCTGAGCAC AGATCATCTCCTGTGCTAGAGATGCTGGAGGAAAGCCAGGAGCTCCTGGAGCCTGTGGTTGGTGATAATG TGGCAGAGACTGCGGCAGAGATGACGTGTAACAGTCTACAAAGTAACAGTCACAGTGACCAGTCCAGAGA CACACAAGCTGGAGCCCAAGATCCAGTGAACAAGAGGAGCAGTTCATATGCTACTAGGAAAGCCATTGAG AGGGAGGATGTGGAGACGGGACTAGATCCGTTGTCTCTTTTAGCCACTGAATGTGTAGAAAAAACGTCTG ATTCTGAAGATAAGTTATTTTCTCCAGTAATTTCACGTAATCTGGCTGATGAAATTGAAAGTTACATGAA CCTAAAAAGCCCACTAGGCAGTAAATCTTGTAGTATGGAATTGCATGGAGAGGGGAACCAGGAGCCTGGC TCTCCTGCTGTGTTTGCTCACCCTTTAGAAAGGAGCTCGAGTCTGCCTTCAGACCGTGGCCCGCCAGCAC GAGACAGCACCGAGACTGAGAAGAGCTCCCCTGCTGTGTCCAGCTCTAAAACTCTGACTGGGCGTTTCAA GCCACAGAGTCCTTACCGCGCTTACAAAGACCGGTCCACTTCCTTATCAGCATTAGTACGTTCTTCACCA AACAGCTCTCTGGGTTCTGTAGTCAATTCTCTATCGGGGCTAAAGTTGGACAATATACTCTCAGGGCCCA AGATAGATGTCCTAAAATCTAGTATGAAACAAGCAGCAACGGTGGCCAGTAAGATGTGGGTGGCTGTAGC ATCTGCCTATAGCTACTCAGATGACGAGGAGGAAACTAATAAAGATTACAGTTTCCCAGCTGGCCTAGAA GACCATCATATAGTGGGAGAGACTCTGTCGCCTAACACAAGTGTCTCAGGGTTGGTTCCCAGTGAACTGA CCCAGAGCAACACAAGCCTCGGCAGTAGTAGCAGCAGTGGAGACGTTGGAAAACTGCAGTGTCCAGCAGG TGAAGTTCCATTTTCAAGAAATATCAAAGGGCAGGACTTTGAAAAGTCAGACCATGGTTCTTCTCAGAAT ACCAGTATGTCTAGCATCTATCAAAATTGTGCGATGGAGGTTTTGATGTCGAGTTGCTCACAGTGCAGAG CTTGTGGCGCTCTGGTTTATGATGAGGAAATCATGGCTGGATGGACAGCAGATGACTCAAATTTGAATAC AACCTGCCCGTTCTGTAAAAGCAACTTCTTGCCTCTTCTCAATGTAGAATTTAAAGACCTGCGAGGCTCT GCCAGCTTTTTCTTGAAACCAAGCACGTCTGGTGACAGTTTACAAAGCGGCAGCATTCCATCAGCCAGTG AGCCCTCTGAACACAAACCCACGTCCAGTTCAGCAGAACCTGACCTGATCAGCTTTATGGATTTCTCAAA ACACAGTGAGACCATAACAGAAGAAGCAAGTTATACAGTCGAATCAAGTGATGAAATAAAGAAAACCAAT GGTGATGTCCAGAGTGTGAAAATGTCATCTGTGCCTAACAGTTTATCTAAGCGAAACGTATCTTTGACTC GAAGTCACAGTGTTGGAGGCCCTCTGCAGAATATCGACTTCTCCCAGCGACCATTTCATGGCGTCTCAAC AGTCAGTCTTCCAAGCAGTCTGCAGGAAGATGTGGACCATCTAGGCAAGCGACCCAGTCCTCCTCCTGTG TCTGTGCCCTATTTGAGTCCTCTAGTGCTTCGGAAGGAACTTGAATCTTTGCTAGAAAATGAAGGTGATC AGGTCATCCATACATCCTCATTCATCAATCAACACCCAATCATTTTCTGGAACCTGGTTTGGTATTTCAG ACGTTTGGACCTTCCTAGCAACTTGCCAGGCCTTATTCTCACCTCTGAGCATTGTAATGGAGGTGTGCAG CTTCCTCTATCATCTTTGTCCCAGGACAGCAAACTGGTGTATATTCAACTGCTGTGGGATAATATCAACC TTCATCAGGAACCAGGAGAACCTCTGTATGTCTCTTGGAGGAACTTGAATTCTGAGAAGAAGCCGTCACT TCTGTCAGAGCAGCAGCAGGCAGCCAGCGCTTTAGTGGAGACGATCAGGCAGAGCATCCAGCAGAATGAC GTTCTCAAGCCCATCAACCTGCTTTCCCAGCAAATGAAGCCAGGCACGAAACGGCAGAGGAGTTTATACA GAGAAATCCTTTTCTTATCATTAGTGTCTCTTGGGAGAGAAAACATTGATATTGAGGCTTTTGACAATGA ATATGGACTCGCATACAGAAGTCTGCCTTCAGAGAGTCTTGAAAGGTTACAGAGAATCGATGCCCCACCC AGTATCAGTGTGGAGTGGTGCAGGAAGTGTTTTGGAGCGCCTCTCATTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_184088
Insert Size	5721 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_184088.1, NP_908976.1
RefSeq Size	7793 bp
RefSeq ORF	5721 bp
Locus ID	329877
UniProt ID	A6H8H2
Gene Summary	Guanine nucleotide exchange factor (GEF) activating RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB10 into its active GTP-bound form. Thereby, stimulates SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane in response to insulin.[UniProtKB/Swiss-Prot Function]

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