Akap5 (NM_001101471) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	3526401B18Rik; AKAP-5; AKAP 150; AKAP150; BB098886; Gm258; P150
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222726 representing NM_001101471, codon optimized. Due to the complexity of NM_001101471, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACTAGCGTCAGTGAAATCCAGGTGGAGACGAAAGACGAGAAGGGCCCCGTTGCCGCCTCCCCAC AGAAGGAGAGGCAGGAGCGGAAGACCGCAACGCTTTGTTTTAAGCGCAGGAAGAAAGCCAACAAAACTAA ACCTAAAGCCGGTTCCAGAACCGCGGAGGAGACAAAGAAGCATACCCCTGAGGCTGGCGGCAGCGGCCAG AGGCAGCCTGCCGGGGCCTGGGCTTCAATAAAGGGCCTCGTGACCCACCGGAAGAGGAGCGAGCCAGCCA AGAAACAGAAGCCTCCAGAGGCTGAGGTCCAGCCAGAGGACGGGGCTTTGCCTAAAAAAAAGGCCAAGTC TAGGCTGAAGTTCCCGTGCCTGAGGTTTTCCCGCGGCGCTAAGAGATCTAGGCACTCAAAGTTGACCGAA GATTCCGGATACGTGCGCGTGCAGGGGGAGGCCGATGATTTGGAGATTAAAGCTCAGACTCAGCCCGACG ACCAAGCCATCCAGGCTGGTTCAACACAGGGACTCCAGGAAGGCGTTCTGGTGCGCGATGGAAAAAAGAG CCAGGAGAGTCATATCAGCAACTCCGTGACAAGCGGCGAGAACGTCATTGCTATTGAACTGGAACTTGAG AATAAGTCATCAGCCATCCAGATGGGAACCCCCGAGCTGGAGAAAGAGACTAAGGTTATTACTGAAAAGC CATCTGTGCAGACACAGCGGGCTAGCCTCCTCGAGAGCTCTGCGGCCGGAAGTCCCCGATCTGTGACTTC TGCCGCTCCACCTAGCCCAGCAACTACCCACCAGCACTCACTGGAGGAGCCCTCCAATGGCATACGGGAA TCTGCCCCATCAGGCAAAGATGACCGGAGGAAAACAGCTGCTGAGGAGAAGAAATCAGGCGAGACAGCCT TGGGTCAAGCAGAGGAAGCCGCTGTGGGCCAGGCTGATAAAAGAGCACTGAGCCAGGCTGGAGAAGCGAC AGCCGGCCACCCAGAGGAGGCCACCGTCATTCAGGCCGAATCACAGGCCAAGGAGGGAAAGTTGTCCCAG GCCGAAGAGACAACAGTAGCACAAGCCAAGGAGACTGTCCTGTCCCAGGCCAAGGAGGGTGAGCTCTCAC AGGCCAAGAAGGCGACAGTAGGTCAGGCCGAGGAGGCCACCATTGACCACACTGAGAAAGTCACTGTAGA CCAGGCTGAAGAGACAACAGTCGGACAAGCAGAGGAGGCGACTGTGGGTCAGGCCGGCGAAGCCATTCTG TCACAAGCCAAGGAAGCAACCGTAGTTGGCCAGGCCGAGGAAGCTACCGTGGACAGGGCAGAGGAAGCTA CCGTGGGCCAGGCAGAGGAGGCGACCGTGGGGCATACAGAAAAGGTTACTGTGGATCAGGCCGAGGAAGC CACTGTGGGCCAGGCCGAGGAGGCGACTGTAGGCCAGGCCGAGGAGGCCACAGTTGACTGGGCCGAGAAG CCAACAGTCGGGCAAGCCGAGGAAGCCACTGTTGGACAAGCAGAAGAGGCCACTGTGGGCCATACTGAAA AGGTTACAGTGGACCAGGCCGAGGAGGCGACCGTGGGACAGGCTGAGGAAGCTACTGTTGGGCATACCGA GAAGGTCACAGTGGATCACGCAGAAGAGGCTACGGTTGGCCAGGCCGAGGAGGCAACGGTAGGGCAGGCG GAGAAGGTCACAGTGGATCATGCCGAGGAAGCAACAGTGGGCCAGGCCGAGGAAGCAACGGTGGGCCAGG CAGAAAAAGTCACTGTGGATCATGCTGAAGAGGCCACTGTGGGTCAGGCAGAGGAAGCCACCGTGGGTCA AGCCGAAAAGGTAACAGTCGATCAGGCCGAGGAGCCCACCGTCGATCAAGCCGAGGAGGCTATCTCTAGC CATGCCCCTGATCTGAAGGAGAACGGCATTGATACAGAGAAGCCGAGATCCGAGGAGTCTAAACGGATGG AACCCATTGCAATAATCATAACCGATACAGAGATCTCCGAGTTCGACGTGAAGAAGAGTAAGAATGTACC AAAGCAGTTCCTGATAAGCATGGAGAATGAGCAGGTCGGCGTGTTTGCCAATGACTCAGATTTTGAAGGA AGGACGAGCGAACAGTACGAGACTCTGCTCATAGAAACTGCCAGTAGTCTCGTTAAGAATGCTATCGAGT TGTCTGTGGAGCAACTTGTTAACGAAATGGTGTCAGAAGATAATCAGATCAATACTCTGTTCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222726 representing NM_001101471 Red=Cloning site Green=Tags(s) METSVSEIQVETKDEKGPVAASPQKERQERKTATLCFKRRKKANKTKPKAGSRTAEETKKHTPEAGGSGQ RQPAGAWASIKGLVTHRKRSEPAKKQKPPEAEVQPEDGALPKKKAKSRLKFPCLRFSRGAKRSRHSKLTE DSGYVRVQGEADDLEIKAQTQPDDQAIQAGSTQGLQEGVLVRDGKKSQESHISNSVTSGENVIAIELELE NKSSAIQMGTPELEKETKVITEKPSVQTQRASLLESSAAGSPRSVTSAAPPSPATTHQHSLEEPSNGIRE SAPSGKDDRRKTAAEEKKSGETALGQAEEAAVGQADKRALSQAGEATAGHPEEATVIQAESQAKEGKLSQ AEETTVAQAKETVLSQAKEGELSQAKKATVGQAEEATIDHTEKVTVDQAEETTVGQAEEATVGQAGEAIL SQAKEATVVGQAEEATVDRAEEATVGQAEEATVGHTEKVTVDQAEEATVGQAEEATVGQAEEATVDWAEK PTVGQAEEATVGQAEEATVGHTEKVTVDQAEEATVGQAEEATVGHTEKVTVDHAEEATVGQAEEATVGQA EKVTVDHAEEATVGQAEEATVGQAEKVTVDHAEEATVGQAEEATVGQAEKVTVDQAEEPTVDQAEEAISS HAPDLKENGIDTEKPRSEESKRMEPIAIIITDTEISEFDVKKSKNVPKQFLISMENEQVGVFANDSDFEG RTSEQYETLLIETASSLVKNAIELSVEQLVNEMVSEDNQINTLFQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001101471
ORF Size	2235 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001101471.1, NP_001094941.1
RefSeq Size	6685 bp
RefSeq ORF	2238 bp
Locus ID	238276
UniProt ID	D3YVF0
MW	79.4 kDa
Gene Summary	May anchor the PKA kinase to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors.[UniProtKB/Swiss-Prot Function]

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