Akap5 (NM_001101471) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3526401B18Rik; AKAP-5; AKAP 150; AKAP150; BB098886; Gm258; P150
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC221335 representing NM_001101471 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCAGCGTTTCTGAGATTCAGGTAGAGACGAAGGACGAGAAGGGACCAGTAGCAGCCAGTCCTC AGAAAGAGAGGCAGGAGAGAAAAACAGCCACACTCTGCTTCAAGAGAAGGAAGAAAGCGAACAAGACAAA ACCCAAAGCCGGTTCCAGGACTGCTGAGGAGACAAAGAAACATACCCCCGAAGCTGGGGGCTCTGGTCAA CGGCAACCAGCGGGTGCCTGGGCCTCCATCAAAGGCCTTGTGACACACAGGAAAAGGTCCGAGCCTGCAA AGAAGCAGAAGCCGCCTGAGGCAGAGGTGCAGCCCGAGGATGGGGCTCTTCCTAAGAAAAAGGCAAAATC CAGACTTAAGTTTCCTTGCCTAAGATTCTCAAGAGGGGCAAAGAGAAGTCGTCATTCCAAACTCACAGAA GATTCAGGCTACGTCAGAGTCCAGGGAGAGGCAGACGATTTGGAGATAAAAGCCCAGACCCAGCCAGATG ACCAGGCAATCCAGGCTGGGTCTACCCAGGGCCTACAGGAAGGTGTGTTGGTGAGAGATGGTAAGAAGAG CCAAGAATCACACATAAGCAACAGCGTCACCTCGGGAGAGAATGTGATCGCCATAGAACTGGAATTAGAA AACAAATCTTCTGCTATCCAGATGGGAACTCCTGAGCTGGAAAAGGAGACTAAGGTGATCACGGAAAAGC CAAGTGTGCAGACGCAGCGAGCAAGTCTACTTGAGAGTTCAGCAGCAGGCAGCCCTCGGTCAGTGACTTC TGCTGCTCCTCCATCACCAGCAACCACACATCAACATAGTCTGGAAGAACCCAGTAATGGCATCCGGGAA AGTGCCCCAAGTGGGAAAGATGACAGGAGAAAGACTGCTGCTGAAGAAAAGAAATCAGGAGAGACTGCAC TGGGCCAGGCAGAGGAAGCTGCAGTTGGCCAGGCAGACAAAAGGGCACTAAGCCAGGCCGGAGAAGCTAC AGCGGGCCACCCAGAGGAAGCTACTGTGATCCAGGCAGAGAGTCAGGCAAAGGAAGGGAAACTGAGCCAG GCAGAGGAAACTACAGTGGCCCAGGCAAAGGAAACTGTATTGAGTCAGGCAAAGGAAGGTGAACTGAGCC AGGCAAAGAAAGCTACAGTTGGCCAGGCAGAGGAAGCTACAATTGACCACACAGAGAAAGTCACAGTGGA TCAGGCAGAGGAAACCACAGTGGGTCAGGCAGAAGAAGCTACAGTTGGTCAGGCAGGGGAAGCTATATTG AGCCAAGCAAAGGAAGCTACAGTTGTTGGTCAGGCAGAGGAAGCTACAGTTGATCGGGCAGAGGAAGCTA CTGTTGGTCAGGCAGAGGAAGCTACAGTTGGCCACACAGAGAAAGTCACAGTGGATCAGGCAGAGGAAGC CACGGTGGGTCAGGCAGAGGAAGCTACTGTTGGTCAGGCAGAGGAAGCTACAGTTGATTGGGCAGAAAAA CCCACAGTTGGTCAGGCAGAGGAAGCCACAGTGGGTCAGGCAGAGGAAGCTACAGTTGGCCACACAGAGA AAGTCACAGTGGATCAGGCAGAGGAAGCAACAGTGGGTCAGGCAGAGGAAGCTACAGTTGGCCACACAGA GAAAGTCACAGTGGATCATGCAGAGGAAGCCACAGTGGGTCAGGCAGAGGAAGCTACAGTTGGACAGGCA GAGAAAGTCACAGTGGATCATGCAGAGGAAGCCACAGTGGGTCAGGCAGAGGAAGCTACAGTTGGACAGG CAGAGAAAGTCACAGTGGATCATGCAGAGGAAGCCACAGTGGGTCAGGCAGAGGAAGCTACAGTTGGACA GGCAGAGAAAGTCACAGTGGATCAGGCAGAGGAACCCACAGTGGATCAGGCTGAGGAAGCTATATCGAGC CATGCACCAGATCTGAAAGAAAATGGAATTGATACAGAGAAACCGAGATCAGAAGAAAGCAAACGAATGG AGCCAATTGCTATTATCATTACTGACACTGAAATCAGTGAATTTGATGTTAAGAAGTCTAAAAATGTTCC TAAGCAATTCCTAATTTCAATGGAAAACGAGCAAGTGGGGGTTTTTGCTAATGATAGTGATTTCGAGGGG AGAACTTCAGAACAGTATGAAACACTCTTAATAGAAACAGCATCTTCTCTCGTCAAGAATGCTATCGAGT TGTCTGTAGAACAGCTGGTTAATGAAATGGTTTCCGAGGATAATCAAATAAATACGCTGTTCCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001101471
Insert Size	2238 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001101471.1, NP_001094941.1
RefSeq Size	6685 bp
RefSeq ORF	2238 bp
Locus ID	238276
UniProt ID	D3YVF0
Gene Summary	May anchor the PKA kinase to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors.[UniProtKB/Swiss-Prot Function]

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