Foxd1 (NM_008242) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI385632; BF-2; FREAC4; Hfh10; Hfhbf2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222088 representing NM_008242, codon optimized. Due to the complexity of NM_008242, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTGAGTACAGAAATGAGTGACGCCTCTGGACTTGCCGAAGAAACAGACATAGATGTGGTGGGCG AAGGAGAGGATGACGAAGAAGAAGAGGATGATGACGATGAGGGAGGAGGTGGGAGAGGGGGCGGCGGCAG CAGACTCCCTTCTTCCGCACAGAGGCGGCGCAGATCTTACGCCGGTGAGGACGACCTGGAAGACTTGGAA GAGGAGGACGACGACGACCTCTTGCTGGCGTCCAGACCTGCAGCAAGCCCTGCTCCTCCAGGTCCCGCAC CAGCTCCCGGCACTGGGAGCGGTGGTTGTTCTGGAGCTGGGGCGGGGGGGGGTGCCGGAGGGGGGACCGG CGCTGGAACAGGTGGAGGTGCAAAAAATCCACTCGTCAAGCCGCCTTACAGCTATATCGCTTTGATCACA ATGGCCATCCTGCAAAGCCCAAAAAAGAGACTCACCCTTTCCGAAATCTGCGAGTTCATATCCAGCCGCT TTCCATACTATCGGGAGAAGTTCCCTGCCTGGCAGAACTCCATCCGCCACAATCTGAGTCTCAATGACTG CTTCGTGAAGATCCCCAGAGAACCAGGCAACCCCGGAAAAGGGAACTATTGGACCCTCGACCCCGAGTCT GCAGATATGTTCGATAATGGCTCCTTTTTGAGGCGCAGGAAAAGATTTAAGAGACAGCCACTGCTGGCCC CTCACGCCGCTGCAGAAGCCCTTCTGCTGAGGGGCGCTGGCCCCGCCGCTGGTGCTGGTGACCCGGGCGC AGCTCTCTTTCCCCCCCCACCCCCCCCCCCCGCCTGTGGCTACGGCGCATACGGTTGCGCCTACGGTCTT CAGCTGCCCCCCTGCGCTCCTCCCAGTGCCCTGTTCGCGGCGGCGGCCGCTGCTGCTGCAGCCGCCTTTC ATCCACATAGTCCTCCACCTCCCCCTCCTCCACCCCCACCTCCACCAGGGGCCGCAGCTGAGTTGGCCAG GACCGCCTTTGGGTATAGATCACATGCATTGGCAGCCGCCCTTCCAGGCCCCCTGCAGGCGGCTGCTGTA AAGGCAGGGGGTCCCGGAGCCGCAGCTCTGGCTCGAAGTCCTTTCTCAATAGAGAGTCTGATCGGTCGAA CGCGGGGCCCGGCTGCCGCCGGAGCACATGTCTCTAGCGGAGCAGCTTCCGGCACTGCCCCAGGACCCGG CGGTGGCGGATGCGCCGTGCAGGCAGCTGCAGGCCCCGCCGTGGCTCTGACTAGATCCCTCGTGGTCGCC GCTGCTGCTGCTGCCTCTTCCGTGTCTAGTAGCGCAGCCTTGGGCACACTTCACCAGGGGACGGCTCTGT CCTCCGTGGAGAATTTCACGGCACGGATTTCCAATTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222088 representing NM_008242 Red=Cloning site Green=Tags(s) MTLSTEMSDASGLAEETDIDVVGEGEDDEEEEDDDDEGGGGRGGGGSRLPSSAQRRRRSYAGEDDLEDLE EEDDDDLLLASRPAASPAPPGPAPAPGTGSGGCSGAGAGGGAGGGTGAGTGGGAKNPLVKPPYSYIALIT MAILQSPKKRLTLSEICEFISSRFPYYREKFPAWQNSIRHNLSLNDCFVKIPREPGNPGKGNYWTLDPES ADMFDNGSFLRRRKRFKRQPLLAPHAAAEALLLRGAGPAAGAGDPGAALFPPPPPPPACGYGAYGCAYGL QLPPCAPPSALFAAAAAAAAAAFHPHSPPPPPPPPPPPPGAAAELARTAFGYRSHALAAALPGPLQAAAV KAGGPGAAALARSPFSIESLIGRTRGPAAAGAHVSSGAASGTAPGPGGGGCAVQAAAGPAVALTRSLVVA AAAAASSVSSSAALGTLHQGTALSSVENFTARISNC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_008242
ORF Size	1368 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_008242.1, NM_008242.2, NP_032268.2
RefSeq Size	2461 bp
RefSeq ORF	1371 bp
Locus ID	15229
UniProt ID	Q61345
MW	45.4 kDa
Gene Summary	Transcription factor involved in regulation of gene expression in a variety of processes including formation of positional identity in the developing retina, regionalization of the optic chiasm, morphogenesis of the kidney, and neuralization of ectodermal cells (PubMed:15509772, PubMed:15634693, PubMed:9811586). Involved in transcriptional activation of PGF and C3 genes (By similarity).[UniProtKB/Swiss-Prot Function]

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