Taf1 (NM_001081008) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	250kDa; AU015687; B430306D02Rik; Ccg-1; Ccg1; KAT4; N-TAF1; Taf2a; TAFII250
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221910 representing NM_001081008 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCGGGGTGGGCTGGGCTGCTGCAGGACAAGGGTGGCGGTAGCCCTTCCGTCGTCATGTCCGACA CAGACAGCGACGAAGAGTCGGCTGGTGGAGGCCCATTTTCTCTAACTGGGTTCCTGTTCGGCAACATCAA TGGAGCAGGGCAGTTGGAAGGCGAAAGCGTCTTGGATGACGAGTGCAAGAAGCATCTTGCGGGCTTGGGG GCTTTGGGTCTTGGCAGCCTGATCACTGAACTCACAGCAAATGAAGAATTGTCCGGGAGTGATGGTGCCC TGGTAAATGATGAAGGATGGATTAGGAGTAGAGAGGATGCTGTGGACTATTCTGACATCAACGAGGTGGC AGAAGATGAAAGTAGAAGATACCAGCAGACAATGGGAAGCTTGCAACCCCTTTGCCACACAGATTATGAT GAAGATGACTATGATGCTGATTGTGAAGATATTGATTGCAAATTGATGCCTCCTCCACCTCCACCCCCAG GACCACTGAAGAAAGAGAAGGACCAAGATGATATTACTGGTGAGAAAGTGGACTTCAGTAGTTCCTCTGA CTCAGAATCTGAGATGGGACCTCAGGACGCAGCACAGTCAGAATCTAAGGATGGACAGCTGACCCTACCA TTGGCTGGGATTATGCAGCATGATGCCACGAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTA GGCCTGGAAAGGTGTTACGCTTTTTACGGCTTTTTGGACCGGGGAAGAATGTTCCATCTGTTTGGAGGAG TGCTCGGAGAAAGAGGAAAAAGAAGCACCGTGAGCTTATACAGGAAGGGCAGGTCCAGGAAGAGGAGTGC TCAGTGGAATTAGAAGTCAACCAGAAATCTCTGTGGAACTATGACTATGCTCCTCCTCCACTTCCAGACC AGTGTCTCTCTGATGATGAAATCACAATGATGGCTCCTGTGGAGTCCAAGTTTTCCCAGTCAACTGGAGA CACAGATAAAGTAATGGATACCAAGCCAAGAGTGGCCGAATGGCGTTATGGACCTGCCCGGCTGTGGTAT GATATGCTAGGTGTTCCTGAAGATGGCAGTGGCTTTGATTATGGCTTCAAAATGAAGAAGACTGAACATG AATCTACAATAAAATGCAACATAATGAAGAAATTAAGGAAACTTGAGGAAAACAGTGGCGTCGATCTTCT GGCAGATGAAAACTTCCTAATGGTGACACAGCTGCATTGGGAAGATGATATCATTTGGGATGGGGAGGAT GTCAAACACAAAGGAACAAAACCTCAGCGTGCGAGTTTGGCAGGCTGGCTTCCTTCCAGTATGACTAGGA ATGCCATGGCCTACAATGTGCAGCAAGGTAGACCCTGTATCAAAAAACCAACTAGCCAAACAAAGCAAAA CACACATACACAAAACTCCATTAGCCAGCCCTCAGGCTCACTTCACACTACATTGGATGATGATAAACCT TGGTACTCCATTTTCCCCATTGATAATGAAGATCTGGTATATGGACGTTGGGAGGACAATATCATTTGGG ATGCTCAGAACATGCCTAGAATTTTGGAGCCTCCTGTTTTGACACTTGATCCCAATGATGAGAACTTGAT TTTGGAGATCCCTGATGAGAAGGAAGAAGCTACTTCTAATTCTCCTTCTAAGGAGAATAAGAAAGAATCA TCTCTGAAGAAGAGTCGAATTCTCTTAGGAAAAACAGGAGTCATCAAGGAAGAGCCACAGCAGAACATGT CTCAGCCAGAAGTGAAAGATCCATGGAATCTCTCCAATGATGAGTATTACTATCCCAAGCAACAGGGCCT TCGAGGTACTTTTGGAGGAAATATTATCCAGCACTCAATTCCAGCTGTAGAATTACGGCAGCCCTTCTTT CCTACCCACATGGGACCCATCAAACTTCGGCAGTTCCATCGCCCACCTCTAAAAAAGTACTCCTTTGGGG CACTATCTCAGCCAGGTCCACACTCAGTTCAGCCTTTGCTGAAGCACATCAAAAAGAAGGCAAAGATGAG AGAACAAGAGAGGCAAGCCTCGGGTGGTGGAGAGATGTTTTTTATGCGCACACCTCAGGATCTCACAGGC AAAGATGGAGACCTTATTCTTGCTGAATACAGTGAGGAAAATGGACCATTAATGATGCAAGTTGGCATGG CAACCAAGATAAAAAACTACTATAAGCGGAAACCTGGAAAAGATCCTGGAGCCCCAGATTGCAAATACGG AGAAACTGTTTATTGCCATACATCGCCTTTCCTGGGTTCTCTTCATCCTGGCCAGTTACTGCAGGCATTT GAGAACAACCTTTTTCGTGCTCCAATTTATCTTCATAAGATGCCAGAATCTGACTTCCTGATCATTCGAA CAAGGCAGGGTTACTTTATTCGAGAATTAGTGGATATTTTTGTTGTTGGCCAGCAGTGTCCCTTATTTGA AGTTCCTGGTCCTAACTCCAAAAGGGCCAATACACACATCCGAGATTTTCTTCAGGTTTTTATTTACCGG CTCTTTTGGAAGAGTAAAGATAGGCCACGGAGGATACGGATGGAAGATATAAAGAAAGCCTTTCCTTCAC ATTCAGAAAGCAGCATCCGGAAGAGGCTAAAGCTCTGTGCCGACTTCAAACGTACAGGGATGGATTCAAA CTGGTGGGTGCTGAAGTCTGATTTTCGTTTACCAACGGAAGAGGAGATCCGAGCTATGGTATCCCCAGAA CAGTGCTGTGCTTATTATAGCATGATAGCTGCTGAACAGAGACTAAAGGATGCTGGCTATGGAGAGAAGT CCTTTTTTGCTCCTGAGGAAGAAAATGAAGAAGATTTCCAGATGAAGATTGATGATGAAGTTCGTACTGC TCCTTGGAATACTACAAGGGCTTTCATTGCTGCCATGAAGGGCAAATGTCTCCTGGAGGTGACTGGAGTA GCAGATCCTACAGGCTGTGGTGAAGGGTTCTCCTATGTGAAGATTCCAAACAAACCGACACAACAGAAGG ATGATAAAGAGCCTCAGCCAGTGAAAAAGACAGTCACAGGAACAGATGCAGACCTTCGTCGCCTGTCTCT GAAAAATGCCAAGCAGCTTCTTCGTAAATTTGGTGTGCCTGAGGAGGAGATTAAAAAGTTGTCCCGCTGG GAAGTTATTGATGTGGTACGTACAATGTCAACAGAACAGGCTCGCTCTGGAGAGGGGCCCATGAGTAAAT TTGCTCGTGGATCAAGGTTTTCAGTGGCTGAGCATCAAGAACGTTACAAAGAGGAATGTCAGCGCATCTT TGACCTACAGAACAAGGTTTTGTCATCAACAGAGGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAA GACAGTGACTTTGAAGAAATGGGAAAGAATATTGAGAATATGCTGCAGAACAAGAAAACAAGTTCTCAGC TGTCTCGGGAGCGGGAGGAGCAGGAACGGAAGGAACTCCAGCGGATGCTACTGGAAGCTGATGGTGAGGC AGCAGGCTCAGCAGCAGCAGGAAACAATCACAGAGATGATGACACAGCTTCAGTGACCAGCCTTAACTCT TCTGCCACTGGCCGCTGTCTCAAGATTTATCGAACATTTCGAGATGAGGAGGGGAAGGAATATGTTCGTT GTGAGACAGTCCGAAAAGCAACTGTTATTGATGCTTATGTACGCATACGGACCACAAAAGATGAAGAGTT CATTCGAAAATTTGCCCTTTTTGATGAGCAACACCGAGAAGAGATGAGAAAAGAACGGCGGAGGATTCAA GAGCAATTGAGGAGACTTAAACGGAACCAGGAAAAGGAAAAGCTAAAGGGGCCTCCTGAGAAAAAGCCCA AAAAAATGAAGGAACGGCCTGACTTAAAATTGAAATGTGGAGCATGTGGTGCCATCGGGCACATGAGGAC AAATAAATTCTGCCCCCTTTATTATCAAACAAATGCTCCACCTTCTAACCCTGTTGCTATGACCGAGGAA CAAGAGGAAGAGCTGGAAAAGACAGTCATTCATAATGATAATGAAGAACTTATCAAGGTTGAAGGGACCA AGATCGTTTTGGGCAAACAGCTAATTGAGAGTGCAGATGAAGTTCGCAGAAAATCTCTGGTCCTGAAGTT CCCTAAACAACAACTTCCCCCCAAGAAAAAACGACGGGTTGGAACCACTGTTCACTGTGACTATTTGAAT AGACCTCATAAATCCATCCACCGGCGCCGGACAGACCCGATGGTGACACTGTCTTCTATCTTGGAGTCTA TTATCAATGACATGAGAGATCTTCCAAATACATACCCTTTCCATACTCCAGTTAATGCAAAGGTTGTAAA GGATTACTATAAAATCATCACTCGACCAATGGATCTACAGACACTCCGTGAAAACGTGCGCAAACGCCTT TATCCGTCTCGGGAAGAATTCAGAGAACATTTGGAGCTAATTGTGAAAAACAGTGCAACTTATAATGGAC CAAAGCACTCACTGACTCAGATTTCTCAATCCATGCTGGATCTCTGCGATGAAAAACTCAAAGAGAAAGA GGATAAGTTGGCACGTTTAGAGAAAGCTATCAACCCCTTGCTTGACGATGATGATCAAGTAGCCTTTTCT TTTATTCTGGATAACATTGTCACTCAGAAAATGATGGCAGTTCCAGATTCTTGGCCATTTCATCACCCAG TTAATAAGAAATTTGTTCCAGATTATTACAAAGTGATTGTCAGTCCAATGGATTTGGAGACTATACGTAA GAATATCTCCAAGCACAAATACCAGAGTCGAGAGAGCTTTCTAGACGACGTAAACCTGATTCTGGCCAAC AGTGTTAAGTACAATGGGCCTGAGAGTCAGTATACTAAGACTGCTCAGGAGATTGTGAACGTTTGTCACC AGACATTAACTGAGTATGATGAACATTTGACTCAACTTGAGAAAGATATTTGTACAGCTAAAGAAGCAGC TCTGGAGGAAGCAGAATTAGAAAGTCTGGACCCAATGACTCCAGGGCCATATACACCTCAGCCTCCTGAT TTGTATGATAACAACACATCCCTCAGTGTTTCTCGAGATGCTTCTGTATATCAAGATGAGAGCAATCTGT CTGTCTTGGATATTCCCAGTGCTACTTCAGAAAAGCAGCTAACACAGGAAGGTGGAGATGGAGATGGTGA TCTTGCAGATGAAGAGGAAGGAACTGTACAACAGCCCCAAGCCAGTGTCTTGTATGAGGATTTGCTTATG TCTGAAGGCGAAGATGATGAGGAAGATGCTGGGAGTGACGAAGAAGGAGATAACCCTTTCTTTGCTATTC AGCTAAGTGAAAGTGGAAGTGACTCTGATGTGGAATCTGGCAGTTTAAGACCCAAACAGCCCCGTGTGCT TCAAGAGAATACAAGGATGGGCATGGAAAATGAAGAAAGCATGATGTCCTATGAGGGAGATGGTGGGGAC GCTTCCCGTGGTTTGGAGGATAGCAACATCAGTTATGGGAGCTATGAGGAGCCTGATCCCAAGTCCAACA CCCAGGATACCAGCTTCAGCAGCATCGGTGGGTATGAGGTATCAGAGGAGGAAGAAGATGAGGAAGAGCA GCGCTCTGGGCCCAGCGTACTAAGCCAGGTCCACCTCTCAGAGGACGAGGAGGACAGTGAGGATTTCCAC TCCATTGCCGGAGACACTGATTTGGACTCTGATGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221910 representing NM_001081008 Red=Cloning site Green=Tags(s) MGPGWAGLLQDKGGGSPSVVMSDTDSDEESAGGGPFSLTGFLFGNINGAGQLEGESVLDDECKKHLAGLG ALGLGSLITELTANEELSGSDGALVNDEGWIRSREDAVDYSDINEVAEDESRRYQQTMGSLQPLCHTDYD EDDYDADCEDIDCKLMPPPPPPPGPLKKEKDQDDITGEKVDFSSSSDSESEMGPQDAAQSESKDGQLTLP LAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEGQVQEEEC SVELEVNQKSLWNYDYAPPPLPDQCLSDDEITMMAPVESKFSQSTGDTDKVMDTKPRVAEWRYGPARLWY DMLGVPEDGSGFDYGFKMKKTEHESTIKCNIMKKLRKLEENSGVDLLADENFLMVTQLHWEDDIIWDGED VKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGRPCIKKPTSQTKQNTHTQNSISQPSGSLHTTLDDDKP WYSIFPIDNEDLVYGRWEDNIIWDAQNMPRILEPPVLTLDPNDENLILEIPDEKEEATSNSPSKENKKES SLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFF PTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTG KDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAF ENNLFRAPIYLHKMPESDFLIIRTRQGYFIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYR LFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPE QCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGV ADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRW EVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAE DSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLEADGEAAGSAAAGNNHRDDDTASVTSLNS SATGRCLKIYRTFRDEEGKEYVRCETVRKATVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQ EQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEE QEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLN RPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRL YPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFS FILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVSPMDLETIRKNISKHKYQSRESFLDDVNLILAN SVKYNGPESQYTKTAQEIVNVCHQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPD LYDNNTSLSVSRDASVYQDESNLSVLDIPSATSEKQLTQEGGDGDGDLADEEEGTVQQPQASVLYEDLLM SEGEDDEEDAGSDEEGDNPFFAIQLSESGSDSDVESGSLRPKQPRVLQENTRMGMENEESMMSYEGDGGD ASRGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEQRSGPSVLSQVHLSEDEEDSEDFH SIAGDTDLDSDE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001081008
ORF Size	5706 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081008.1, NP_001074477.1
RefSeq Size	8060 bp
RefSeq ORF	5709 bp
Locus ID	270627
MW	216.3 kDa
Gene Summary	Largest component and core scaffold of the TFIID basal transcription factor complex (PubMed:10438527). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.