Taf1 (NM_001081008) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	250kDa; AU015687; B430306D02Rik; Ccg-1; Ccg1; KAT4; N-TAF1; Taf2a; TAFII250
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225024 representing NM_001081008 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCGGGGTGGGCTGGGCTGCTGCAGGACAAGGGTGGCGGTAGCCCTTCCGTCGTCATGTCCGACA CAGACAGCGACGAAGAGTCGGCTGGTGGAGGCCCATTTTCTCTAACTGGGTTCCTGTTCGGCAACATCAA TGGAGCAGGGCAGTTGGAAGGCGAAAGCGTCTTGGATGACGAGTGCAAGAAGCATCTTGCGGGCTTGGGG GCTTTGGGTCTTGGCAGCCTGATCACTGAACTCACAGCAAATGAAGAATTGTCCGGGAGTGATGGTGCCC TGGTAAATGATGAAGGATGGATTAGGAGTAGAGAGGATGCTGTGGACTATTCTGACATCAACGAGGTGGC AGAAGATGAAAGTAGAAGATACCAGCAGACAATGGGAAGCTTGCAACCCCTTTGCCACACAGATTATGAT GAAGATGACTATGATGCTGATTGTGAAGATATTGATTGCAAATTGATGCCTCCTCCACCTCCACCCCCAG GACCACTGAAGAAAGAGAAGGACCAAGATGATATTACTGGTGAGAAAGTGGACTTCAGTAGTTCCTCTGA CTCAGAATCTGAGATGGGACCTCAGGACGCAGCACAGTCAGAATCTAAGGATGGACAGCTGACCCTACCA TTGGCTGGGATTATGCAGCATGATGCCACGAAGCTGTTGCCAAGTGTCACAGAACTTTTTCCAGAATTTA GGCCTGGAAAGGTGTTACGCTTTTTACGGCTTTTTGGACCGGGGAAGAATGTTCCATCTGTTTGGAGGAG TGCTCGGAGAAAGAGGAAAAAGAAGCACCGTGAGCTTATACAGGAAGGGCAGGTCCAGGAAGAGGAGTGC TCAGTGGAATTAGAAGTCAACCAGAAATCTCTGTGGAACTATGACTATGCTCCTCCTCCACTTCCAGACC AGTGTCTCTCTGATGATGAAATCACAATGATGGCTCCTGTGGAGTCCAAGTTTTCCCAGTCAACTGGAGA CACAGATAAAGTAATGGATACCAAGCCAAGAGTGGCCGAATGGCGTTATGGACCTGCCCGGCTGTGGTAT GATATGCTAGGTGTTCCTGAAGATGGCAGTGGCTTTGATTATGGCTTCAAAATGAAGAAGACTGAACATG AATCTACAATAAAATGCAACATAATGAAGAAATTAAGGAAACTTGAGGAAAACAGTGGCGTCGATCTTCT GGCAGATGAAAACTTCCTAATGGTGACACAGCTGCATTGGGAAGATGATATCATTTGGGATGGGGAGGAT GTCAAACACAAAGGAACAAAACCTCAGCGTGCGAGTTTGGCAGGCTGGCTTCCTTCCAGTATGACTAGGA ATGCCATGGCCTACAATGTGCAGCAAGGTAGACCCTGTATCAAAAAACCAACTAGCCAAACAAAGCAAAA CACACATACACAAAACTCCATTAGCCAGCCCTCAGGCTCACTTCACACTACATTGGATGATGATAAACCT TGGTACTCCATTTTCCCCATTGATAATGAAGATCTGGTATATGGACGTTGGGAGGACAATATCATTTGGG ATGCTCAGAACATGCCTAGAATTTTGGAGCCTCCTGTTTTGACACTTGATCCCAATGATGAGAACTTGAT TTTGGAGATCCCTGATGAGAAGGAAGAAGCTACTTCTAATTCTCCTTCTAAGGAGAATAAGAAAGAATCA TCTCTGAAGAAGAGTCGAATTCTCTTAGGAAAAACAGGAGTCATCAAGGAAGAGCCACAGCAGAACATGT CTCAGCCAGAAGTGAAAGATCCATGGAATCTCTCCAATGATGAGTATTACTATCCCAAGCAACAGGGCCT TCGAGGTACTTTTGGAGGAAATATTATCCAGCACTCAATTCCAGCTGTAGAATTACGGCAGCCCTTCTTT CCTACCCACATGGGACCCATCAAACTTCGGCAGTTCCATCGCCCACCTCTAAAAAAGTACTCCTTTGGGG CACTATCTCAGCCAGGTCCACACTCAGTTCAGCCTTTGCTGAAGCACATCAAAAAGAAGGCAAAGATGAG AGAACAAGAGAGGCAAGCCTCGGGTGGTGGAGAGATGTTTTTTATGCGCACACCTCAGGATCTCACAGGC AAAGATGGAGACCTTATTCTTGCTGAATACAGTGAGGAAAATGGACCATTAATGATGCAAGTTGGCATGG CAACCAAGATAAAAAACTACTATAAGCGGAAACCTGGAAAAGATCCTGGAGCCCCAGATTGCAAATACGG AGAAACTGTTTATTGCCATACATCGCCTTTCCTGGGTTCTCTTCATCCTGGCCAGTTACTGCAGGCATTT GAGAACAACCTTTTTCGTGCTCCAATTTATCTTCATAAGATGCCAGAATCTGACTTCCTGATCATTCGAA CAAGGCAGGGTTACTTTATTCGAGAATTAGTGGATATTTTTGTTGTTGGCCAGCAGTGTCCCTTATTTGA AGTTCCTGGTCCTAACTCCAAAAGGGCCAATACACACATCCGAGATTTTCTTCAGGTTTTTATTTACCGG CTCTTTTGGAAGAGTAAAGATAGGCCACGGAGGATACGGATGGAAGATATAAAGAAAGCCTTTCCTTCAC ATTCAGAAAGCAGCATCCGGAAGAGGCTAAAGCTCTGTGCCGACTTCAAACGTACAGGGATGGATTCAAA CTGGTGGGTGCTGAAGTCTGATTTTCGTTTACCAACGGAAGAGGAGATCCGAGCTATGGTATCCCCAGAA CAGTGCTGTGCTTATTATAGCATGATAGCTGCTGAACAGAGACTAAAGGATGCTGGCTATGGAGAGAAGT CCTTTTTTGCTCCTGAGGAAGAAAATGAAGAAGATTTCCAGATGAAGATTGATGATGAAGTTCGTACTGC TCCTTGGAATACTACAAGGGCTTTCATTGCTGCCATGAAGGGCAAATGTCTCCTGGAGGTGACTGGAGTA GCAGATCCTACAGGCTGTGGTGAAGGGTTCTCCTATGTGAAGATTCCAAACAAACCGACACAACAGAAGG ATGATAAAGAGCCTCAGCCAGTGAAAAAGACAGTCACAGGAACAGATGCAGACCTTCGTCGCCTGTCTCT GAAAAATGCCAAGCAGCTTCTTCGTAAATTTGGTGTGCCTGAGGAGGAGATTAAAAAGTTGTCCCGCTGG GAAGTTATTGATGTGGTACGTACAATGTCAACAGAACAGGCTCGCTCTGGAGAGGGGCCCATGAGTAAAT TTGCTCGTGGATCAAGGTTTTCAGTGGCTGAGCATCAAGAACGTTACAAAGAGGAATGTCAGCGCATCTT TGACCTACAGAACAAGGTTTTGTCATCAACAGAGGTCTTATCAACTGACACAGACAGCAGCTCAGCTGAA GACAGTGACTTTGAAGAAATGGGAAAGAATATTGAGAATATGCTGCAGAACAAGAAAACAAGTTCTCAGC TGTCTCGGGAGCGGGAGGAGCAGGAACGGAAGGAACTCCAGCGGATGCTACTGGAAGCTGATGGTGAGGC AGCAGGCTCAGCAGCAGCAGGAAACAATCACAGAGATGATGACACAGCTTCAGTGACCAGCCTTAACTCT TCTGCCACTGGCCGCTGTCTCAAGATTTATCGAACATTTCGAGATGAGGAGGGGAAGGAATATGTTCGTT GTGAGACAGTCCGAAAAGCAACTGTTATTGATGCTTATGTACGCATACGGACCACAAAAGATGAAGAGTT CATTCGAAAATTTGCCCTTTTTGATGAGCAACACCGAGAAGAGATGAGAAAAGAACGGCGGAGGATTCAA GAGCAATTGAGGAGACTTAAACGGAACCAGGAAAAGGAAAAGCTAAAGGGGCCTCCTGAGAAAAAGCCCA AAAAAATGAAGGAACGGCCTGACTTAAAATTGAAATGTGGAGCATGTGGTGCCATCGGGCACATGAGGAC AAATAAATTCTGCCCCCTTTATTATCAAACAAATGCTCCACCTTCTAACCCTGTTGCTATGACCGAGGAA CAAGAGGAAGAGCTGGAAAAGACAGTCATTCATAATGATAATGAAGAACTTATCAAGGTTGAAGGGACCA AGATCGTTTTGGGCAAACAGCTAATTGAGAGTGCAGATGAAGTTCGCAGAAAATCTCTGGTCCTGAAGTT CCCTAAACAACAACTTCCCCCCAAGAAAAAACGACGGGTTGGAACCACTGTTCACTGTGACTATTTGAAT AGACCTCATAAATCCATCCACCGGCGCCGGACAGACCCGATGGTGACACTGTCTTCTATCTTGGAGTCTA TTATCAATGACATGAGAGATCTTCCAAATACATACCCTTTCCATACTCCAGTTAATGCAAAGGTTGTAAA GGATTACTATAAAATCATCACTCGACCAATGGATCTACAGACACTCCGTGAAAACGTGCGCAAACGCCTT TATCCGTCTCGGGAAGAATTCAGAGAACATTTGGAGCTAATTGTGAAAAACAGTGCAACTTATAATGGAC CAAAGCACTCACTGACTCAGATTTCTCAATCCATGCTGGATCTCTGCGATGAAAAACTCAAAGAGAAAGA GGATAAGTTGGCACGTTTAGAGAAAGCTATCAACCCCTTGCTTGACGATGATGATCAAGTAGCCTTTTCT TTTATTCTGGATAACATTGTCACTCAGAAAATGATGGCAGTTCCAGATTCTTGGCCATTTCATCACCCAG TTAATAAGAAATTTGTTCCAGATTATTACAAAGTGATTGTCAGTCCAATGGATTTGGAGACTATACGTAA GAATATCTCCAAGCACAAATACCAGAGTCGAGAGAGCTTTCTAGACGACGTAAACCTGATTCTGGCCAAC AGTGTTAAGTACAATGGGCCTGAGAGTCAGTATACTAAGACTGCTCAGGAGATTGTGAACGTTTGTCACC AGACATTAACTGAGTATGATGAACATTTGACTCAACTTGAGAAAGATATTTGTACAGCTAAAGAAGCAGC TCTGGAGGAAGCAGAATTAGAAAGTCTGGACCCAATGACTCCAGGGCCATATACACCTCAGCCTCCTGAT TTGTATGATAACAACACATCCCTCAGTGTTTCTCGAGATGCTTCTGTATATCAAGATGAGAGCAATCTGT CTGTCTTGGATATTCCCAGTGCTACTTCAGAAAAGCAGCTAACACAGGAAGGTGGAGATGGAGATGGTGA TCTTGCAGATGAAGAGGAAGGAACTGTACAACAGCCCCAAGCCAGTGTCTTGTATGAGGATTTGCTTATG TCTGAAGGCGAAGATGATGAGGAAGATGCTGGGAGTGACGAAGAAGGAGATAACCCTTTCTTTGCTATTC AGCTAAGTGAAAGTGGAAGTGACTCTGATGTGGAATCTGGCAGTTTAAGACCCAAACAGCCCCGTGTGCT TCAAGAGAATACAAGGATGGGCATGGAAAATGAAGAAAGCATGATGTCCTATGAGGGAGATGGTGGGGAC GCTTCCCGTGGTTTGGAGGATAGCAACATCAGTTATGGGAGCTATGAGGAGCCTGATCCCAAGTCCAACA CCCAGGATACCAGCTTCAGCAGCATCGGTGGGTATGAGGTATCAGAGGAGGAAGAAGATGAGGAAGAGCA GCGCTCTGGGCCCAGCGTACTAAGCCAGGTCCACCTCTCAGAGGACGAGGAGGACAGTGAGGATTTCCAC TCCATTGCCGGAGACACTGATTTGGACTCTGATGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081008
Insert Size	5709 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081008.1, NP_001074477.1
RefSeq Size	8060 bp
RefSeq ORF	5709 bp
Locus ID	270627
Gene Summary	Largest component and core scaffold of the TFIID basal transcription factor complex (PubMed:10438527). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4.[UniProtKB/Swiss-Prot Function]

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