Rbm25 (NM_027349) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2600011C06Rik; 2610015J01Rik; A130095G20Rik; AI159652; AL023075; AU043498; RNPC7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR217225 representing NM_027349 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTCCCCCCTCATTTGAATCGCCCTCCCATGGGAATCCCAGCTCTCCCTCCAGGGATCCCACCCC CACAGTTTCCTGGCTTTCCTCCACCAGTACCTCCAGGGACACCGATGATTCCTGTACCAATGAGTATTAT GGCTCCTGCTCCAACTGTCCTAGTACCTACCGTGTCCATGGTTGGAAAGCATTTGGGAGCCAGAAAGGAT CATCCAGGCTTAAAATTAAAAGAAAATGATGAAAATTGTGGCCCTACTACTACAGTTTTTGTTGGGAACA TTTCTGAGAAGGCTTCAGACATGCTTATACGACAGCTCTTAGCTAAATGTGGTTTGGTGTTGAGCTGGAA GAGAGTGCAAGGTGCTTCTGGGAAACTTCAAGCTTTTGGATTTTGTGAGTATAAAGAGCCTGAATCTACC CTCCGTGCTCTCAGATTATTGCATGACCTTCAGATTGGAGAGAAGAAGCTACTTGTTAAAGTTGATGCCA AGACAAAGGCACAGTTGGATGAATGGAAAGCAAAGAAGAAAGCTAATGGGAACGCCAGACCAGAAACTGT CACTAATGATGATGAAGAAGCCTTAGATGAAGAAACAAAGCGAAGAGACCAGATGATTAAAGGGGCCATT GAAGTTTTAATCCGAGAATATTCCAGTGAACTAAATGCCCCCTCACAGGAGTCTGACTCTCACCCCAGAA AGAAGAAAAAGGAAAAGAAGGAGGACATTTTCCGTAGATTTCCAGTGGCCCCATTGATCCCTTACCCACT CATCACCAAGGAGGATATAAATGCTATTGAAATGGAAGAAGACAAAAGAGATTTGATATCCCGAGAGATC AGCAAATTCAGAGATACACACAAGAAACTGGAAGAAGAGAAAGGCAAAAAAGAAAAAGAAAGACAGGAAA TTGAGAAAGAACGGAGAGAAAGAGAGAGGGAACGTGAAAGGGAACGAGAAAGGCGAGAACGGGAACGAGA AAGGGAAAGAGAACGTGAACGAGAAAAGGAAAAAGAACGGGAGCGGGAACGAGAACGGGATAGGGATCGT GACCGCACAAAAGAGAGAGACCGAGATCGAGAGAGAGATCGGGACCGAGATCGAGAACGGAGCTCAGATC GAAATAAGGATCGGAGTCGATCAAGAGAAAAAAGCAGAGATCGTGAAAGGGAACGGGAGCGGGAAAGAGA GAGAGAACGAGAACGGGAACGAGAACGAGAGCGAGAGAGAGAACGGGAGAGAGAACGGGAACGGGAAAGA GAGAAAGACAAGAAAAGAGACAGAGAAGAGGATGAAGAAGATGCATATGAACGAAGAAAACTCGAACGAA AACTGCGAGAGAAAGAGGCTGCTTATCAAGAGCGCCTTAAGAATTGGGAAATCAGAGAACGAAAGAAAAC TAGGGAATATGAGAAGGAGGCAGAAAGAGAAGAAGAAAGAAGAAGAGAAATGGCTAAGGAGGCTAAACGA CTAAAAGAATTCCTAGAAGATTATGATGATGATAGAGATGACCCGAAGTACTACAGGGGTAGTGCTCTTC AGAAACGGTTGCGTGATAGGGAAAAAGAAATGGAAGCTGATGAACGAGACAGGAAGAGAGAGAAGGAAGA GCTTGAGGAAATCAGGCAGCGCTTACTGGCAGAAGGGCATCCAGATCCAGATGCAGAGCTCCAGAGGATG GAACAAGAGGCTGAGAGGCGCAGGCAACCTCAAATAAAGCAAGAACCAGAATCCGAGGAAGAAGAAGAAG AAAAGCAAGAAAAAGAAGAAAAACGAGAGGAGCCTGTGGAAGAGGAGGAAGAACCAGAGCAAAAGCCTTG TCTCAAGCCGACTTTGAGACCCATCAGCTCTGCCCCATCTGTGTCCTCTGCCAGTGGCAACGCAACCCCC AACACTCCTGGGGATGAGTCTCCTTGTGGTATTATTATTCCTCATGAAAACTCACCAGATCAACAGCAGC CTGAGGAGCATAGGCCAAAAATAGGACTAAGCCTTAAATTGGGTGCTTCCAATAGTCCTGGCCAACCTAA TTCAGTGAAGAGAAAGAAACTACCTGTAGATAGTGTCTTTAACAAATTCGAGGATGAGGACAGTGATGAT GTGCCCCGAAAAAGGAAACTCGTTCCCCTGGATTATGGCGAAGATGATAAAAATGCCACCAAAGGCACTG TAAACACTGAAGAAAAGCGCAAACACATTAAGAGTCTCATTGAGAAAATTCCCACAGCCAAACCTGAGCT CTTTGCTTATCCTTTGGATTGGTCTATTGTGGATTCTATACTGATGGAACGAAGAATCCGGCCATGGATC AATAAGAAAATCATCGAATACATAGGTGAAGAAGAAGCTACATTAGTTGATTTTGTTTGCTCTAAGGTTA TGGCACATAGTTCACCACAGAGCATTTTAGATGATGTTGCCATGGTACTAGATGAAGAAGCAGAAGTTTT TATAGTCAAAATGTGGAGATTATTGATATATGAAACAGAAGCCAAGAAAATTGGCCTTGTGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR217225 representing NM_027349 Red=Cloning site Green=Tags(s) MSFPPHLNRPPMGIPALPPGIPPPQFPGFPPPVPPGTPMIPVPMSIMAPAPTVLVPTVSMVGKHLGARKD HPGLKLKENDENCGPTTTVFVGNISEKASDMLIRQLLAKCGLVLSWKRVQGASGKLQAFGFCEYKEPEST LRALRLLHDLQIGEKKLLVKVDAKTKAQLDEWKAKKKANGNARPETVTNDDEEALDEETKRRDQMIKGAI EVLIREYSSELNAPSQESDSHPRKKKKEKKEDIFRRFPVAPLIPYPLITKEDINAIEMEEDKRDLISREI SKFRDTHKKLEEEKGKKEKERQEIEKERRERERERERERERREREREREREREREKEKERERERERDRDR DRTKERDRDRERDRDRDRERSSDRNKDRSRSREKSRDRERERERERERERERERERERERERERERERER EKDKKRDREEDEEDAYERRKLERKLREKEAAYQERLKNWEIRERKKTREYEKEAEREEERRREMAKEAKR LKEFLEDYDDDRDDPKYYRGSALQKRLRDREKEMEADERDRKREKEELEEIRQRLLAEGHPDPDAELQRM EQEAERRRQPQIKQEPESEEEEEEKQEKEEKREEPVEEEEEPEQKPCLKPTLRPISSAPSVSSASGNATP NTPGDESPCGIIIPHENSPDQQQPEEHRPKIGLSLKLGASNSPGQPNSVKRKKLPVDSVFNKFEDEDSDD VPRKRKLVPLDYGEDDKNATKGTVNTEEKRKHIKSLIEKIPTAKPELFAYPLDWSIVDSILMERRIRPWI NKKIIEYIGEEEATLVDFVCSKVMAHSSPQSILDDVAMVLDEEAEVFIVKMWRLLIYETEAKKIGLVK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_027349
ORF Size	2514 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_027349.4
RefSeq Size	4244 bp
RefSeq ORF	2517 bp
Locus ID	67039
UniProt ID	B2RY56
MW	99.6 kDa
Gene Summary	RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function]

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