Rbm25 (NM_027349) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2600011C06Rik; 2610015J01Rik; A130095G20Rik; AI159652; AL023075; AU043498; RNPC7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC222005 representing NM_027349 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTCCCCCCTCATTTGAATCGCCCTCCCATGGGAATCCCAGCTCTCCCTCCAGGGATCCCACCCC CACAGTTTCCTGGCTTTCCTCCACCAGTACCTCCAGGGACACCGATGATTCCTGTACCAATGAGTATTAT GGCTCCTGCTCCAACTGTCCTAGTACCTACCGTGTCCATGGTTGGAAAGCATTTGGGAGCCAGAAAGGAT CATCCAGGCTTAAAATTAAAAGAAAATGATGAAAATTGTGGCCCTACTACTACAGTTTTTGTTGGGAACA TTTCTGAGAAGGCTTCAGACATGCTTATACGACAGCTCTTAGCTAAATGTGGTTTGGTGTTGAGCTGGAA GAGAGTGCAAGGTGCTTCTGGGAAACTTCAAGCTTTTGGATTTTGTGAGTATAAAGAGCCTGAATCTACC CTCCGTGCTCTCAGATTATTGCATGACCTTCAGATTGGAGAGAAGAAGCTACTTGTTAAAGTTGATGCCA AGACAAAGGCACAGTTGGATGAATGGAAAGCAAAGAAGAAAGCTAATGGGAACGCCAGACCAGAAACTGT CACTAATGATGATGAAGAAGCCTTAGATGAAGAAACAAAGCGAAGAGACCAGATGATTAAAGGGGCCATT GAAGTTTTAATCCGAGAATATTCCAGTGAACTAAATGCCCCCTCACAGGAGTCTGACTCTCACCCCAGAA AGAAGAAAAAGGAAAAGAAGGAGGACATTTTCCGTAGATTTCCAGTGGCCCCATTGATCCCTTACCCACT CATCACCAAGGAGGATATAAATGCTATTGAAATGGAAGAAGACAAAAGAGATTTGATATCCCGAGAGATC AGCAAATTCAGAGATACACACAAGAAACTGGAAGAAGAGAAAGGCAAAAAAGAAAAAGAAAGACAGGAAA TTGAGAAAGAACGGAGAGAAAGAGAGAGGGAACGTGAAAGGGAACGAGAAAGGCGAGAACGGGAACGAGA AAGGGAAAGAGAACGTGAACGAGAAAAGGAAAAAGAACGGGAGCGGGAACGAGAACGGGATAGGGATCGT GACCGCACAAAAGAGAGAGACCGAGATCGAGAGAGAGATCGGGACCGAGATCGAGAACGGAGCTCAGATC GAAATAAGGATCGGAGTCGATCAAGAGAAAAAAGCAGAGATCGTGAAAGGGAACGGGAGCGGGAAAGAGA GAGAGAACGAGAACGGGAACGAGAACGAGAGCGAGAGAGAGAACGGGAGAGAGAACGGGAACGGGAAAGA GAGAAAGACAAGAAAAGAGACAGAGAAGAGGATGAAGAAGATGCATATGAACGAAGAAAACTCGAACGAA AACTGCGAGAGAAAGAGGCTGCTTATCAAGAGCGCCTTAAGAATTGGGAAATCAGAGAACGAAAGAAAAC TAGGGAATATGAGAAGGAGGCAGAAAGAGAAGAAGAAAGAAGAAGAGAAATGGCTAAGGAGGCTAAACGA CTAAAAGAATTCCTAGAAGATTATGATGATGATAGAGATGACCCGAAGTACTACAGGGGTAGTGCTCTTC AGAAACGGTTGCGTGATAGGGAAAAAGAAATGGAAGCTGATGAACGAGACAGGAAGAGAGAGAAGGAAGA GCTTGAGGAAATCAGGCAGCGCTTACTGGCAGAAGGGCATCCAGATCCAGATGCAGAGCTCCAGAGGATG GAACAAGAGGCTGAGAGGCGCAGGCAACCTCAAATAAAGCAAGAACCAGAATCCGAGGAAGAAGAAGAAG AAAAGCAAGAAAAAGAAGAAAAACGAGAGGAGCCTGTGGAAGAGGAGGAAGAACCAGAGCAAAAGCCTTG TCTCAAGCCGACTTTGAGACCCATCAGCTCTGCCCCATCTGTGTCCTCTGCCAGTGGCAACGCAACCCCC AACACTCCTGGGGATGAGTCTCCTTGTGGTATTATTATTCCTCATGAAAACTCACCAGATCAACAGCAGC CTGAGGAGCATAGGCCAAAAATAGGACTAAGCCTTAAATTGGGTGCTTCCAATAGTCCTGGCCAACCTAA TTCAGTGAAGAGAAAGAAACTACCTGTAGATAGTGTCTTTAACAAATTCGAGGATGAGGACAGTGATGAT GTGCCCCGAAAAAGGAAACTCGTTCCCCTGGATTATGGCGAAGATGATAAAAATGCCACCAAAGGCACTG TAAACACTGAAGAAAAGCGCAAACACATTAAGAGTCTCATTGAGAAAATTCCCACAGCCAAACCTGAGCT CTTTGCTTATCCTTTGGATTGGTCTATTGTGGATTCTATACTGATGGAACGAAGAATCCGGCCATGGATC AATAAGAAAATCATCGAATACATAGGTGAAGAAGAAGCTACATTAGTTGATTTTGTTTGCTCTAAGGTTA TGGCACATAGTTCACCACAGAGCATTTTAGATGATGTTGCCATGGTACTAGATGAAGAAGCAGAAGTTTT TATAGTCAAAATGTGGAGATTATTGATATATGAAACAGAAGCCAAGAAAATTGGCCTTGTGAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_027349
Insert Size	2517 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_027349.3, NP_081625.3
RefSeq Size	4244 bp
RefSeq ORF	2517 bp
Locus ID	67039
UniProt ID	B2RY56
Gene Summary	RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function]

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