Rbm25 (NM_027349) Mouse Untagged Clone

CAT#: MC222005

Rbm25 (untagged) - Mouse RNA binding motif protein 25 (Rbm25), (10ug)



  "NM_027349" in other vectors (2)

CNY 10,740.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Rbm25 Antibody - middle region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2600011C06Rik; 2610015J01Rik; A130095G20Rik; AI159652; AL023075; AU043498; RNPC7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222005 representing NM_027349
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTTCCCCCCTCATTTGAATCGCCCTCCCATGGGAATCCCAGCTCTCCCTCCAGGGATCCCACCCC
CACAGTTTCCTGGCTTTCCTCCACCAGTACCTCCAGGGACACCGATGATTCCTGTACCAATGAGTATTAT
GGCTCCTGCTCCAACTGTCCTAGTACCTACCGTGTCCATGGTTGGAAAGCATTTGGGAGCCAGAAAGGAT
CATCCAGGCTTAAAATTAAAAGAAAATGATGAAAATTGTGGCCCTACTACTACAGTTTTTGTTGGGAACA
TTTCTGAGAAGGCTTCAGACATGCTTATACGACAGCTCTTAGCTAAATGTGGTTTGGTGTTGAGCTGGAA
GAGAGTGCAAGGTGCTTCTGGGAAACTTCAAGCTTTTGGATTTTGTGAGTATAAAGAGCCTGAATCTACC
CTCCGTGCTCTCAGATTATTGCATGACCTTCAGATTGGAGAGAAGAAGCTACTTGTTAAAGTTGATGCCA
AGACAAAGGCACAGTTGGATGAATGGAAAGCAAAGAAGAAAGCTAATGGGAACGCCAGACCAGAAACTGT
CACTAATGATGATGAAGAAGCCTTAGATGAAGAAACAAAGCGAAGAGACCAGATGATTAAAGGGGCCATT
GAAGTTTTAATCCGAGAATATTCCAGTGAACTAAATGCCCCCTCACAGGAGTCTGACTCTCACCCCAGAA
AGAAGAAAAAGGAAAAGAAGGAGGACATTTTCCGTAGATTTCCAGTGGCCCCATTGATCCCTTACCCACT
CATCACCAAGGAGGATATAAATGCTATTGAAATGGAAGAAGACAAAAGAGATTTGATATCCCGAGAGATC
AGCAAATTCAGAGATACACACAAGAAACTGGAAGAAGAGAAAGGCAAAAAAGAAAAAGAAAGACAGGAAA
TTGAGAAAGAACGGAGAGAAAGAGAGAGGGAACGTGAAAGGGAACGAGAAAGGCGAGAACGGGAACGAGA
AAGGGAAAGAGAACGTGAACGAGAAAAGGAAAAAGAACGGGAGCGGGAACGAGAACGGGATAGGGATCGT
GACCGCACAAAAGAGAGAGACCGAGATCGAGAGAGAGATCGGGACCGAGATCGAGAACGGAGCTCAGATC
GAAATAAGGATCGGAGTCGATCAAGAGAAAAAAGCAGAGATCGTGAAAGGGAACGGGAGCGGGAAAGAGA
GAGAGAACGAGAACGGGAACGAGAACGAGAGCGAGAGAGAGAACGGGAGAGAGAACGGGAACGGGAAAGA
GAGAAAGACAAGAAAAGAGACAGAGAAGAGGATGAAGAAGATGCATATGAACGAAGAAAACTCGAACGAA
AACTGCGAGAGAAAGAGGCTGCTTATCAAGAGCGCCTTAAGAATTGGGAAATCAGAGAACGAAAGAAAAC
TAGGGAATATGAGAAGGAGGCAGAAAGAGAAGAAGAAAGAAGAAGAGAAATGGCTAAGGAGGCTAAACGA
CTAAAAGAATTCCTAGAAGATTATGATGATGATAGAGATGACCCGAAGTACTACAGGGGTAGTGCTCTTC
AGAAACGGTTGCGTGATAGGGAAAAAGAAATGGAAGCTGATGAACGAGACAGGAAGAGAGAGAAGGAAGA
GCTTGAGGAAATCAGGCAGCGCTTACTGGCAGAAGGGCATCCAGATCCAGATGCAGAGCTCCAGAGGATG
GAACAAGAGGCTGAGAGGCGCAGGCAACCTCAAATAAAGCAAGAACCAGAATCCGAGGAAGAAGAAGAAG
AAAAGCAAGAAAAAGAAGAAAAACGAGAGGAGCCTGTGGAAGAGGAGGAAGAACCAGAGCAAAAGCCTTG
TCTCAAGCCGACTTTGAGACCCATCAGCTCTGCCCCATCTGTGTCCTCTGCCAGTGGCAACGCAACCCCC
AACACTCCTGGGGATGAGTCTCCTTGTGGTATTATTATTCCTCATGAAAACTCACCAGATCAACAGCAGC
CTGAGGAGCATAGGCCAAAAATAGGACTAAGCCTTAAATTGGGTGCTTCCAATAGTCCTGGCCAACCTAA
TTCAGTGAAGAGAAAGAAACTACCTGTAGATAGTGTCTTTAACAAATTCGAGGATGAGGACAGTGATGAT
GTGCCCCGAAAAAGGAAACTCGTTCCCCTGGATTATGGCGAAGATGATAAAAATGCCACCAAAGGCACTG
TAAACACTGAAGAAAAGCGCAAACACATTAAGAGTCTCATTGAGAAAATTCCCACAGCCAAACCTGAGCT
CTTTGCTTATCCTTTGGATTGGTCTATTGTGGATTCTATACTGATGGAACGAAGAATCCGGCCATGGATC
AATAAGAAAATCATCGAATACATAGGTGAAGAAGAAGCTACATTAGTTGATTTTGTTTGCTCTAAGGTTA
TGGCACATAGTTCACCACAGAGCATTTTAGATGATGTTGCCATGGTACTAGATGAAGAAGCAGAAGTTTT
TATAGTCAAAATGTGGAGATTATTGATATATGAAACAGAAGCCAAGAAAATTGGCCTTGTGAAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_027349
Insert Size 2517 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_027349.3, NP_081625.3
RefSeq Size 4244 bp
RefSeq ORF 2517 bp
Locus ID 67039
UniProt ID B2RY56
Gene Summary RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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