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Hpf1 (NM_028299) Mouse Tagged ORF Clone
CAT＃: MR212701
- TrueORF®
2700029M09Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2700029M09 gene (2700029M09Rik)
ORF Plasmid: tGFP
"NM_028299" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2700029M09Rik; C4orf27; C230006B22Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212701 representing NM_028299
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGGCGGTGGCGGGAAGCGCCGGACGGCCGGGGCGGGACCGCAGTGTGAAAAGACAGTTGAGGTGA AGAAGAGTAAGTTTTCTGAGGCTGATGTCTCCAGTGACCTTCGAAAAGAAGTGGAAAATCTTTATAAGCT TTCACTGCCTGAAGATTTCTATCATTTCTGGAAGTTCTGTGAAGAGCTTGATCCTGAAAAGCCAGCTGAT GCGCTTGCCACAAGCCTGGGTCTTCGGTTAGTGGGTCCTTATGATATCCTTGCTGGGAAACATAAAATGA AGAAAAAACCCACAGGCTTGAACTGTAATCTTCACTGGAGGTTTTACTATGACCCTCCTGAGTTCCAGAC CATTATTATTGGAGATAATAAAACTCAGTACCACATGGGGTATTTCAGGGATTCTCCTGATGAACTTCCT GTATACGTGGGTACAAATGAAGCAAAGAAAAATTGTATAATTATTCAAAATGGAGACAATGTGTTTGCTG CCATCAAATTATTTTTAATGAAAAAACTCAAAGAAGTAACAGATAGAAAGAAAATTAGCATTTTGAAAAA CATAGATGAAAAACTCACAGAAGCAGCCAGAAAACTAGGATACTCACTGGAACAGAGAACCGTGAAGATG AGACAGAGAGATAAGAAGGTTGTGACAAAGACTTTTCATGGTGCAGGCTTGGTTGTTCCAGTAGATAAAA ATGATGTTGGTTACCGAGAGCTCCCTGAAACAGATGCTGACCTTAAGAGAATCTGCAAGGCAGTTGTCGA CGCTGCAAGCGACGAGGAGAGACTGAAAGCATTCGCTCCCATTCAGGAGATGATGACTTTTGTGCAGTTT GCTAATGATGAGTGTGATTATGGCATGGGGCTGGAATTAGGAATGGACCTCTTTTGCTATGGCTCTCATT ATTTTCACAAAGTTGCTGGTCAGCTTTTACCTCTTGCGTATAATCTATTGAAGAGGGATCTGTTTGCAAA AATTATTGAAGATCATCTGGCAAGCAGAAGTGAAGAGAACATAGACCAGCTTGCAGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212701 representing NM_028299
Red=Cloning site Green=Tags(s) MVGGGGKRRTAGAGPQCEKTVEVKKSKFSEADVSSDLRKEVENLYKLSLPEDFYHFWKFCEELDPEKPAD ALATSLGLRLVGPYDILAGKHKMKKKPTGLNCNLHWRFYYDPPEFQTIIIGDNKTQYHMGYFRDSPDELP VYVGTNEAKKNCIIIQNGDNVFAAIKLFLMKKLKEVTDRKKISILKNIDEKLTEAARKLGYSLEQRTVKM RQRDKKVVTKTFHGAGLVVPVDKNDVGYRELPETDADLKRICKAVVDAASDEERLKAFAPIQEMMTFVQF ANDECDYGMGLELGMDLFCYGSHYFHKVAGQLLPLAYNLLKRDLFAKIIEDHLASRSEENIDQLAG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_028299 |
| ORF Size | 1038 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_028299.3 |
| RefSeq Size | 1210 bp |
| RefSeq ORF | 1041 bp |
| Locus ID | 72612 |
| UniProt ID | Q8CFE2 |
| MW | 39.3 kDa |
| Gene Summary | Acts as a cofactor for serine ADP-ribosylation by conferring serine specificity on PARP1 and PARP2: interacts with PARP1 and PARP2 and is able to change amino acid specificity toward serine. Promotes histone serine ADP-ribosylation in response to DNA damage, limiting DNA damage-induced PARP1 hyper-automodification, and ensuring genome stability. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. HPF1 also promotes tyrosine ADP-ribosylation, probably by conferring tyrosine specificity on PARP1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211346 | 2700029M09Rik (untagged) - Mouse RIKEN cDNA 2700029M09 gene (2700029M09Rik), (10ug) |
CNY 3,990.00 |
|
| MG212701 | 2700029M09Rik (tGFP-tagged) - Mouse RIKEN cDNA 2700029M09 gene (2700029M09Rik), (10ug) |
CNY 3,710.00 |
|
| MR212701L3 | Lenti ORF clone of 2700029M09Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2700029M09 gene (2700029M09Rik) |
CNY 5,230.00 |
|
| MR212701L4 | Lenti ORF clone of 2700029M09Rik (mGFP-tagged) - Mouse RIKEN cDNA 2700029M09 gene (2700029M09Rik) |
CNY 5,230.00 |
