Mast2 (BC060703) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MAST205; Mtssk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212074 representing BC060703 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTACTGGACTTAGTCCTCTGCTCTTCAGGAAGCTTAGTAATCCTGACATATTTGCACCCACTGGAA AAGTTAAACTCCAGCGACAACTTAGTCAGGATGACTGTAAGTTACGGAGAGGAAGCCTGGCAAGTTCTCT GTCGGGTAAGCAGCTGCTCCCTTTGTCCAGCAGTGTACACAGCAGTGTGGGACAGGTAACTTGGCAGTCT ACAGGAGAAGCATCAAACCTGGTTCGAATGAGAAACCAATCCCTTGGACAGTCTGCACCTTCCCTTACGG CTGGCTTGAAGGAATTGAGCCTTCCAAGGAGAGGCAGCTTTTGTCGGACAAGTAACCGCAAGAGCTTGAT TGTAACCTCCAGCACATCACCTACGCTACCAAGGCCACACTCCCCACTCCATGGCCACACAGGTAACAGT CCCTTGGACAGCCCCCGGAATTTCTCTCCAAATGCTCCTGCTCACTTTTCCTTCGTTCCTGCCCGTAGCC ATGGCCACAGAACAGACAGGACTGATGGACGGCGCTGGTCTTTGGCCTCGTTGCCTTCTTCAGGCTATGG AACCAACACTCCTAGCTCTACAGTCTCTTCATCATGTTCCTCACAAGAAAAGCTTCATCAGTTACCTTTC CAGCCAACAGCTGATGAACTACACTTTCTGACGAAGCATTTTAGCACAGAAAACGTACCAGATGAGGAGG GACGTCGGTCCCCAGCCATGCGGCCCCGTTCCCGCAGCCTCAGTCCTGGACGGTCCCCAGTTTCCTTTGA CAGTGAAATAATAATGATGAATCATGTGTACAAAGAAAGATTCCCCAAGGCCACTGCACAGATGGAAGAG CGACTAGCTGACTTCATTTCCTCTAACACTCCAGATAGTGTGTTGCCCTTGGCAGATGGAGCACTAAGCT TTATTCATCATCAGGTGATTGAGATGGCCCGAGACTGCCTGGATAAATCTCGGAGTGGCCTCATTACGTC ACACTATTTCTATGAACTTCAAGAGAATTTGGAAAAGCTTCTGCAAGATGCTCACGAACGCTCAGAAAGC TCAGATGTAGCCTTTGTGATACAGCTGGTAAAAAAGTTGATGATCATCATTGCTCGCCCAGCTCGCCTCC TGGAATGCCTGGAGTTTGACCCTGAAGAATTTTACCACCTGTTAGAAGCAGCTGAAGGTCATGCCAAAGA GGGACATGGAATTAAATGTGACATTCCCCGCTACATCGTTAGCCAGCTGGGCCTAACTCGGGATCCCTTG GAGGAAATGGCCCAGTTGAGCAGCTATGACAGTCCAGATACTCCAGAGACAGATGATTCAGTTGAGGGTC GTGGGGTATCTCAGCCATCTCAGAAGACCCCCTCTGAAGAGGACTTTGAAACCATTAAGCTCATCAGCAA TGGCGCCTATGGGGCTGTCTTTCTGGTGCGGCACAAGTCCACGCGGCAGCGCTTTGCAATGAAGAAGATT AATAAGCAAAACCTAATCCTACGGAACCAGATCCAGCAAGCATTTGTGGAACGCGACATACTGACTTTCG CTGAAAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGAGACCAAGCGTCACTTATGCATGGTGATGGA ATACGTAGAAGGGGGAGACTGTGCCACTCTGCTCAAGAACATCGGGGCCCTACCTGTGGACATGGTACGC CTGTACTTTGCGGAAACTGTGCTGGCTTTGGAATACTTACACAACTACGGCATCGTGCACCGTGACCTCA AGCCTGACAACCTTCTGATTACATCCATGGGACACATCAAACTCACTGACTTTGGACTTTCTAAAATCGG CCTCATGAGTTTGACAACCAACTTGTATGAGGGTCATATTGAAAAGGATGCCCGGGAGTTCCTAGACAAG CAGGTATGCGGGACCCCGGAATACATAGCACCTGAGGTGATCCTGCGTCAGGGATATGGGAAGCCAGTGG ACTGGTGGGCCATGGGCATAATCCTGTATGAGTTCCTGGTGGGTTGCGTCCCTTTCTTTGGAGACACTCC GGAGGAGCTCTTTGGGCAAGTGATCAGTGATGAGATCGTGTGGCCCGAAGGTGATGACGCGCTTCCCCCA GATGCCCAAGACCTCACTTCCAAACTGCTTCATCAGAATCCACTAGAAAGACTGGGCACAAGTAGTGCCT ATGAAGTGAAGCAGCACCCATTCTTCATGGGTCTGGACTGGACAGGACTTCTACGGCAGAAGGCTGAATT TATACCTCAGCTGGAGTCAGAGGATGACACCAGCTACTTTGATACCCGTTCAGAACGATACCACCATGTG GACTCTGAGGATGAGGAGGAAGTGAGTGAGGATGGCTGCCTTGAGATTCGCCAGTTTTCTTCCTGCTCTC CAAGGTTCAGCAAGGTTTACAGTAGCATGGAACGGCTTTCCCTGCTTGAGGAACGCCGGACACCACCTCC AACCAAGCGCAGCCTCAGTGAGGAAAAGGAAGATCACTCAGACGGCTTGGCAGGACTGAAGGGCCGAGAT CGCAGCTGGGTGATTGGGTCCCCTGAGATATTACGGAAGCGGTTATCTGTGTCCGAGTCATCCCACACAG AGAGTGATTCGAGTCCTCCAATGACAGTGCGACATCGGTGTTCAGGCCTCCCAGATGGGCCTCATTGCCC TGAGGAGACTAGTAGCACCCCTCGGAAGCAACAACAGGAAGGTATATGGGTCCTCATTCCCCCATCTGGA GAGGGGTCATCTAGGCCTGTTCCTGAACGACCGTTGGAGAGGCAACTGAAGCTGGATGAGGAGCCTCCTG GCCAAAGCAGTCGGTGTTGCCCAGCCCTGGAGACACGAGGCCGTGGGACCCCTCAGCTAGCTGAGGAAGC TACAGCCAAAGCCATCAGCGACCTAGCTGTGCGTAGGGCCCGTCACCGGCTGCTCTCTGGGGACTCTATA GAGAAGCGCACCACTCGCCCTGTCAACAAAGTAATCAAGTCAGCCTCAGCTACGGCCCTTTCCCTCCTCA TTCCTTCAGAACACCATGCCTGCTCACCATTGGCCAGCCCTATGTCCCCACATTCCCAGTCATCCAATCC ATCATCCAGGGACTCTTCTCCAAGCAGGGACTTCTTGCCAGCCCTTGGCAGCTTGAGGCCTCCCATCATC ATCCACCGAGCTGGCAAGAAGTATGGCTTCACCCTGCGGGCCATTCGAGTCTACATGGGTGACACTGATG TCTACACCGTACACCACATGGTGTGGCATGTGGAGGATGGTGGTCCAGCCAGTGAAGCAGGGCTTCGTCA GGGTGACCTCATCACCCATGTCAATGGTGAGCCTGTGCATGGGCTAGTCCACACAGAAGTGGTGGAGCTG GTTCTGAAGAGTGGAAACAAGGTATCAATTTCAACAACTCCCTTGGAGAACACGTCAATCAAAGTGGGGC CAGCTAGGAAAGGCAGCTATAAAGCCAAGATGGCCCGAAGGAGCAAACGGAGCAAAGGCAAGGATGGGCA AGAAAGCCGAAAAAGAAGCTCCCTATTCCGGAAAATCACAAAGCAGGCCTCCTTGCTCCACACCAGCCGC AGCCTTTCTTCCCTTAACCGCTCCTTGTCATCAGGGGAGAGTGGTCCAGGCTCTCCCACACACAGCCACA GCCTCTCTCCCAGATCTCCTCCTCAGGGATACCGGGTAGCCCCAGATGCTGTGCACTCAGGAGGGAATTC CTCGCAGAGCAGCTCTCCCAGCTCCAGTGTGCCCAGTTCTCCTGCTGGCTCTGGACATACACGGCCCAGC TCTCTTCACGGTCTGGCACCCAAGCTCCAACGCCAATACCGCTCACCACGGCGCAAGTCAGCAGGCAGCA TCCCACTGTCACCGTTGGCCCACACCCCTTCCCCACCAGCAACGGCAGCTTCACCTCAGCGTTCCCCATC ACCCTTGTCTGGCCATGGGTCTCAGTCCTTTCCTACCAAACTTCACTTGTCTCCTCCGCTAGGTAGGCAG CTCTCACGGCCCAAGAGTGCAGAGCCACCCCGCTCTCCCCTACTTAAGAGGGTGCAGTCTGCTGAGAAGC TGGCGGCTGCACTGGCAGCTGCTGAGAAGAAGTTAGCACCTTCCCGCAAACATAGTCTTGACCTGCCCCA TGGTGAACTAAAGAAGGAACTGACACCCAGGGAAGCCAGCCCTCTGGAGGTAGTTGGAACCAGAAGTGTG CTATCCGGGAAAGGGCCACTTCCAGGAAAGGGGGTACTGCAGCCTGCTCCTTCACGGGCCCTTGGGACCC TACGGCAGGATCGAGCTGAACGCCGTGAGTCACTGCAAAAACAAGAAGCAATCCGGGAAGTAGACTCCTC AGAAGATGACACTGATGAGGAGCCTGAGAACAGCCAGGCCACACAGGAGCCAAGATTGTCCCCCCACCCA GAAGCAAGCCACAATCTACTCCCTAAAGGTTCAGGAGAGGGTACAGAAGAGGACACTTTCTTGCACAGGG ATCTAAAGAAGCAGGGCCCTGTACTCTCAGGTCTAGTGACAGGGGCCACACTAGGCTCCCCCCGAGTAGA CGTTCCTGGGCTCTCCCCAAGGAAGCTCAGCAGGCCACAAGCCTTTGAGGAAGCTACCAACCCCTTACAA GTCCCTAGCCTGAGCAGGTCTGGACCCACAAGCCCCACCCCCTCTGAAGGCTGCTGGAAGGCCCAGCACC TCCACACACAGGCACTAACTGCACTTTGTCCCAGCTTTTCAGAACTTACCCCTACCGGTTGTTCTGCTGC CACCTCCACCTCTGGAAAGCCAGGGACATGGTCCTGGAAATTCCTTATTGAGGGTCCAGACAGAGCATCC ACGAACAAGACCATAACAAGGAAAGGTGAACCAGCTAACTCCCAAGATACGAATACCACGGTCCCAAATC TTCTGAAGAACCTGTCTCCTGAGGAGGAGAAGCCACAGCCACCAAGTGTGCCTGGGCTGACCCATCCGCT TCTTGAGGTCCCCAGCCAGAACTGGCCATGGGAGTCTGAATGTGAACAAATGGAGAAAGAAGAACCATCC CTGAGCATCACCGAAGTGCCTGATTCCTCAGGCGACAGGAGGCAGGACATTCCATGCAGAGCCCACCCCC TGAGCCCAGAAACCCGGCCCAGCCTGCTCTGGAAAAGCCAAGAACTTGGGGGCCAGCAAGATCATCAGGA CTTAGCACTGACATCAGATGAGCTCTTAAAGCAAACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212074 representing BC060703 Red=Cloning site Green=Tags(s) MVTGLSPLLFRKLSNPDIFAPTGKVKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSSSVHSSVGQVTWQS TGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNS PLDSPRNFSPNAPAHFSFVPARSHGHRTDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPF QPTADELHFLTKHFSTENVPDEEGRRSPAMRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEE RLADFISSNTPDSVLPLADGALSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSES SDVAFVIQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGHGIKCDIPRYIVSQLGLTRDPL EEMAQLSSYDSPDTPETDDSVEGRGVSQPSQKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKI NKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVR LYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDK QVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPP DAQDLTSKLLHQNPLERLGTSSAYEVKQHPFFMGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHV DSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDHSDGLAGLKGRD RSWVIGSPEILRKRLSVSESSHTESDSSPPMTVRHRCSGLPDGPHCPEETSSTPRKQQQEGIWVLIPPSG EGSSRPVPERPLERQLKLDEEPPGQSSRCCPALETRGRGTPQLAEEATAKAISDLAVRRARHRLLSGDSI EKRTTRPVNKVIKSASATALSLLIPSEHHACSPLASPMSPHSQSSNPSSRDSSPSRDFLPALGSLRPPII IHRAGKKYGFTLRAIRVYMGDTDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVEL VLKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSKGKDGQESRKRSSLFRKITKQASLLHTSR SLSSLNRSLSSGESGPGSPTHSHSLSPRSPPQGYRVAPDAVHSGGNSSQSSSPSSSVPSSPAGSGHTRPS SLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPATAASPQRSPSPLSGHGSQSFPTKLHLSPPLGRQ LSRPKSAEPPRSPLLKRVQSAEKLAAALAAAEKKLAPSRKHSLDLPHGELKKELTPREASPLEVVGTRSV LSGKGPLPGKGVLQPAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEEPENSQATQEPRLSPHP EASHNLLPKGSGEGTEEDTFLHRDLKKQGPVLSGLVTGATLGSPRVDVPGLSPRKLSRPQAFEEATNPLQ VPSLSRSGPTSPTPSEGCWKAQHLHTQALTALCPSFSELTPTGCSAATSTSGKPGTWSWKFLIEGPDRAS TNKTITRKGEPANSQDTNTTVPNLLKNLSPEEEKPQPPSVPGLTHPLLEVPSQNWPWESECEQMEKEEPS LSITEVPDSSGDRRQDIPCRAHPLSPETRPSLLWKSQELGGQQDHQDLALTSDELLKQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC060703
ORF Size	5217 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC060703.1
RefSeq Size	5315 bp
RefSeq ORF	5219 bp
Locus ID	17776
MW	194.8 kDa
Gene Summary	Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation.[UniProtKB/Swiss-Prot Function]

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