Mast2 (BC060703) Mouse Tagged ORF Clone

CAT#: MG212074

  • TrueORF®

Mast2 (tGFP-tagged) - Mouse microtubule associated serine/threonine kinase 2 (cDNA clone MGC:67101 IMAGE:6412811)

ORF Plasmid: DDK tGFP



  "BC060703" in other vectors (2)

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Mouse Protein / Lysate Request

CNY 19,100.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms MAST205; Mtssk
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG212074 representing BC060703
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTACTGGACTTAGTCCTCTGCTCTTCAGGAAGCTTAGTAATCCTGACATATTTGCACCCACTGGAA
AAGTTAAACTCCAGCGACAACTTAGTCAGGATGACTGTAAGTTACGGAGAGGAAGCCTGGCAAGTTCTCT
GTCGGGTAAGCAGCTGCTCCCTTTGTCCAGCAGTGTACACAGCAGTGTGGGACAGGTAACTTGGCAGTCT
ACAGGAGAAGCATCAAACCTGGTTCGAATGAGAAACCAATCCCTTGGACAGTCTGCACCTTCCCTTACGG
CTGGCTTGAAGGAATTGAGCCTTCCAAGGAGAGGCAGCTTTTGTCGGACAAGTAACCGCAAGAGCTTGAT
TGTAACCTCCAGCACATCACCTACGCTACCAAGGCCACACTCCCCACTCCATGGCCACACAGGTAACAGT
CCCTTGGACAGCCCCCGGAATTTCTCTCCAAATGCTCCTGCTCACTTTTCCTTCGTTCCTGCCCGTAGCC
ATGGCCACAGAACAGACAGGACTGATGGACGGCGCTGGTCTTTGGCCTCGTTGCCTTCTTCAGGCTATGG
AACCAACACTCCTAGCTCTACAGTCTCTTCATCATGTTCCTCACAAGAAAAGCTTCATCAGTTACCTTTC
CAGCCAACAGCTGATGAACTACACTTTCTGACGAAGCATTTTAGCACAGAAAACGTACCAGATGAGGAGG
GACGTCGGTCCCCAGCCATGCGGCCCCGTTCCCGCAGCCTCAGTCCTGGACGGTCCCCAGTTTCCTTTGA
CAGTGAAATAATAATGATGAATCATGTGTACAAAGAAAGATTCCCCAAGGCCACTGCACAGATGGAAGAG
CGACTAGCTGACTTCATTTCCTCTAACACTCCAGATAGTGTGTTGCCCTTGGCAGATGGAGCACTAAGCT
TTATTCATCATCAGGTGATTGAGATGGCCCGAGACTGCCTGGATAAATCTCGGAGTGGCCTCATTACGTC
ACACTATTTCTATGAACTTCAAGAGAATTTGGAAAAGCTTCTGCAAGATGCTCACGAACGCTCAGAAAGC
TCAGATGTAGCCTTTGTGATACAGCTGGTAAAAAAGTTGATGATCATCATTGCTCGCCCAGCTCGCCTCC
TGGAATGCCTGGAGTTTGACCCTGAAGAATTTTACCACCTGTTAGAAGCAGCTGAAGGTCATGCCAAAGA
GGGACATGGAATTAAATGTGACATTCCCCGCTACATCGTTAGCCAGCTGGGCCTAACTCGGGATCCCTTG
GAGGAAATGGCCCAGTTGAGCAGCTATGACAGTCCAGATACTCCAGAGACAGATGATTCAGTTGAGGGTC
GTGGGGTATCTCAGCCATCTCAGAAGACCCCCTCTGAAGAGGACTTTGAAACCATTAAGCTCATCAGCAA
TGGCGCCTATGGGGCTGTCTTTCTGGTGCGGCACAAGTCCACGCGGCAGCGCTTTGCAATGAAGAAGATT
AATAAGCAAAACCTAATCCTACGGAACCAGATCCAGCAAGCATTTGTGGAACGCGACATACTGACTTTCG
CTGAAAACCCCTTTGTGGTCAGCATGTTCTGCTCCTTTGAGACCAAGCGTCACTTATGCATGGTGATGGA
ATACGTAGAAGGGGGAGACTGTGCCACTCTGCTCAAGAACATCGGGGCCCTACCTGTGGACATGGTACGC
CTGTACTTTGCGGAAACTGTGCTGGCTTTGGAATACTTACACAACTACGGCATCGTGCACCGTGACCTCA
AGCCTGACAACCTTCTGATTACATCCATGGGACACATCAAACTCACTGACTTTGGACTTTCTAAAATCGG
CCTCATGAGTTTGACAACCAACTTGTATGAGGGTCATATTGAAAAGGATGCCCGGGAGTTCCTAGACAAG
CAGGTATGCGGGACCCCGGAATACATAGCACCTGAGGTGATCCTGCGTCAGGGATATGGGAAGCCAGTGG
ACTGGTGGGCCATGGGCATAATCCTGTATGAGTTCCTGGTGGGTTGCGTCCCTTTCTTTGGAGACACTCC
GGAGGAGCTCTTTGGGCAAGTGATCAGTGATGAGATCGTGTGGCCCGAAGGTGATGACGCGCTTCCCCCA
GATGCCCAAGACCTCACTTCCAAACTGCTTCATCAGAATCCACTAGAAAGACTGGGCACAAGTAGTGCCT
ATGAAGTGAAGCAGCACCCATTCTTCATGGGTCTGGACTGGACAGGACTTCTACGGCAGAAGGCTGAATT
TATACCTCAGCTGGAGTCAGAGGATGACACCAGCTACTTTGATACCCGTTCAGAACGATACCACCATGTG
GACTCTGAGGATGAGGAGGAAGTGAGTGAGGATGGCTGCCTTGAGATTCGCCAGTTTTCTTCCTGCTCTC
CAAGGTTCAGCAAGGTTTACAGTAGCATGGAACGGCTTTCCCTGCTTGAGGAACGCCGGACACCACCTCC
AACCAAGCGCAGCCTCAGTGAGGAAAAGGAAGATCACTCAGACGGCTTGGCAGGACTGAAGGGCCGAGAT
CGCAGCTGGGTGATTGGGTCCCCTGAGATATTACGGAAGCGGTTATCTGTGTCCGAGTCATCCCACACAG
AGAGTGATTCGAGTCCTCCAATGACAGTGCGACATCGGTGTTCAGGCCTCCCAGATGGGCCTCATTGCCC
TGAGGAGACTAGTAGCACCCCTCGGAAGCAACAACAGGAAGGTATATGGGTCCTCATTCCCCCATCTGGA
GAGGGGTCATCTAGGCCTGTTCCTGAACGACCGTTGGAGAGGCAACTGAAGCTGGATGAGGAGCCTCCTG
GCCAAAGCAGTCGGTGTTGCCCAGCCCTGGAGACACGAGGCCGTGGGACCCCTCAGCTAGCTGAGGAAGC
TACAGCCAAAGCCATCAGCGACCTAGCTGTGCGTAGGGCCCGTCACCGGCTGCTCTCTGGGGACTCTATA
GAGAAGCGCACCACTCGCCCTGTCAACAAAGTAATCAAGTCAGCCTCAGCTACGGCCCTTTCCCTCCTCA
TTCCTTCAGAACACCATGCCTGCTCACCATTGGCCAGCCCTATGTCCCCACATTCCCAGTCATCCAATCC
ATCATCCAGGGACTCTTCTCCAAGCAGGGACTTCTTGCCAGCCCTTGGCAGCTTGAGGCCTCCCATCATC
ATCCACCGAGCTGGCAAGAAGTATGGCTTCACCCTGCGGGCCATTCGAGTCTACATGGGTGACACTGATG
TCTACACCGTACACCACATGGTGTGGCATGTGGAGGATGGTGGTCCAGCCAGTGAAGCAGGGCTTCGTCA
GGGTGACCTCATCACCCATGTCAATGGTGAGCCTGTGCATGGGCTAGTCCACACAGAAGTGGTGGAGCTG
GTTCTGAAGAGTGGAAACAAGGTATCAATTTCAACAACTCCCTTGGAGAACACGTCAATCAAAGTGGGGC
CAGCTAGGAAAGGCAGCTATAAAGCCAAGATGGCCCGAAGGAGCAAACGGAGCAAAGGCAAGGATGGGCA
AGAAAGCCGAAAAAGAAGCTCCCTATTCCGGAAAATCACAAAGCAGGCCTCCTTGCTCCACACCAGCCGC
AGCCTTTCTTCCCTTAACCGCTCCTTGTCATCAGGGGAGAGTGGTCCAGGCTCTCCCACACACAGCCACA
GCCTCTCTCCCAGATCTCCTCCTCAGGGATACCGGGTAGCCCCAGATGCTGTGCACTCAGGAGGGAATTC
CTCGCAGAGCAGCTCTCCCAGCTCCAGTGTGCCCAGTTCTCCTGCTGGCTCTGGACATACACGGCCCAGC
TCTCTTCACGGTCTGGCACCCAAGCTCCAACGCCAATACCGCTCACCACGGCGCAAGTCAGCAGGCAGCA
TCCCACTGTCACCGTTGGCCCACACCCCTTCCCCACCAGCAACGGCAGCTTCACCTCAGCGTTCCCCATC
ACCCTTGTCTGGCCATGGGTCTCAGTCCTTTCCTACCAAACTTCACTTGTCTCCTCCGCTAGGTAGGCAG
CTCTCACGGCCCAAGAGTGCAGAGCCACCCCGCTCTCCCCTACTTAAGAGGGTGCAGTCTGCTGAGAAGC
TGGCGGCTGCACTGGCAGCTGCTGAGAAGAAGTTAGCACCTTCCCGCAAACATAGTCTTGACCTGCCCCA
TGGTGAACTAAAGAAGGAACTGACACCCAGGGAAGCCAGCCCTCTGGAGGTAGTTGGAACCAGAAGTGTG
CTATCCGGGAAAGGGCCACTTCCAGGAAAGGGGGTACTGCAGCCTGCTCCTTCACGGGCCCTTGGGACCC
TACGGCAGGATCGAGCTGAACGCCGTGAGTCACTGCAAAAACAAGAAGCAATCCGGGAAGTAGACTCCTC
AGAAGATGACACTGATGAGGAGCCTGAGAACAGCCAGGCCACACAGGAGCCAAGATTGTCCCCCCACCCA
GAAGCAAGCCACAATCTACTCCCTAAAGGTTCAGGAGAGGGTACAGAAGAGGACACTTTCTTGCACAGGG
ATCTAAAGAAGCAGGGCCCTGTACTCTCAGGTCTAGTGACAGGGGCCACACTAGGCTCCCCCCGAGTAGA
CGTTCCTGGGCTCTCCCCAAGGAAGCTCAGCAGGCCACAAGCCTTTGAGGAAGCTACCAACCCCTTACAA
GTCCCTAGCCTGAGCAGGTCTGGACCCACAAGCCCCACCCCCTCTGAAGGCTGCTGGAAGGCCCAGCACC
TCCACACACAGGCACTAACTGCACTTTGTCCCAGCTTTTCAGAACTTACCCCTACCGGTTGTTCTGCTGC
CACCTCCACCTCTGGAAAGCCAGGGACATGGTCCTGGAAATTCCTTATTGAGGGTCCAGACAGAGCATCC
ACGAACAAGACCATAACAAGGAAAGGTGAACCAGCTAACTCCCAAGATACGAATACCACGGTCCCAAATC
TTCTGAAGAACCTGTCTCCTGAGGAGGAGAAGCCACAGCCACCAAGTGTGCCTGGGCTGACCCATCCGCT
TCTTGAGGTCCCCAGCCAGAACTGGCCATGGGAGTCTGAATGTGAACAAATGGAGAAAGAAGAACCATCC
CTGAGCATCACCGAAGTGCCTGATTCCTCAGGCGACAGGAGGCAGGACATTCCATGCAGAGCCCACCCCC
TGAGCCCAGAAACCCGGCCCAGCCTGCTCTGGAAAAGCCAAGAACTTGGGGGCCAGCAAGATCATCAGGA
CTTAGCACTGACATCAGATGAGCTCTTAAAGCAAACC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG212074 representing BC060703
Red=Cloning site Green=Tags(s)

MVTGLSPLLFRKLSNPDIFAPTGKVKLQRQLSQDDCKLRRGSLASSLSGKQLLPLSSSVHSSVGQVTWQS
TGEASNLVRMRNQSLGQSAPSLTAGLKELSLPRRGSFCRTSNRKSLIVTSSTSPTLPRPHSPLHGHTGNS
PLDSPRNFSPNAPAHFSFVPARSHGHRTDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPF
QPTADELHFLTKHFSTENVPDEEGRRSPAMRPRSRSLSPGRSPVSFDSEIIMMNHVYKERFPKATAQMEE
RLADFISSNTPDSVLPLADGALSFIHHQVIEMARDCLDKSRSGLITSHYFYELQENLEKLLQDAHERSES
SDVAFVIQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGHGIKCDIPRYIVSQLGLTRDPL
EEMAQLSSYDSPDTPETDDSVEGRGVSQPSQKTPSEEDFETIKLISNGAYGAVFLVRHKSTRQRFAMKKI
NKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETKRHLCMVMEYVEGGDCATLLKNIGALPVDMVR
LYFAETVLALEYLHNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDAREFLDK
QVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEIVWPEGDDALPP
DAQDLTSKLLHQNPLERLGTSSAYEVKQHPFFMGLDWTGLLRQKAEFIPQLESEDDTSYFDTRSERYHHV
DSEDEEEVSEDGCLEIRQFSSCSPRFSKVYSSMERLSLLEERRTPPPTKRSLSEEKEDHSDGLAGLKGRD
RSWVIGSPEILRKRLSVSESSHTESDSSPPMTVRHRCSGLPDGPHCPEETSSTPRKQQQEGIWVLIPPSG
EGSSRPVPERPLERQLKLDEEPPGQSSRCCPALETRGRGTPQLAEEATAKAISDLAVRRARHRLLSGDSI
EKRTTRPVNKVIKSASATALSLLIPSEHHACSPLASPMSPHSQSSNPSSRDSSPSRDFLPALGSLRPPII
IHRAGKKYGFTLRAIRVYMGDTDVYTVHHMVWHVEDGGPASEAGLRQGDLITHVNGEPVHGLVHTEVVEL
VLKSGNKVSISTTPLENTSIKVGPARKGSYKAKMARRSKRSKGKDGQESRKRSSLFRKITKQASLLHTSR
SLSSLNRSLSSGESGPGSPTHSHSLSPRSPPQGYRVAPDAVHSGGNSSQSSSPSSSVPSSPAGSGHTRPS
SLHGLAPKLQRQYRSPRRKSAGSIPLSPLAHTPSPPATAASPQRSPSPLSGHGSQSFPTKLHLSPPLGRQ
LSRPKSAEPPRSPLLKRVQSAEKLAAALAAAEKKLAPSRKHSLDLPHGELKKELTPREASPLEVVGTRSV
LSGKGPLPGKGVLQPAPSRALGTLRQDRAERRESLQKQEAIREVDSSEDDTDEEPENSQATQEPRLSPHP
EASHNLLPKGSGEGTEEDTFLHRDLKKQGPVLSGLVTGATLGSPRVDVPGLSPRKLSRPQAFEEATNPLQ
VPSLSRSGPTSPTPSEGCWKAQHLHTQALTALCPSFSELTPTGCSAATSTSGKPGTWSWKFLIEGPDRAS
TNKTITRKGEPANSQDTNTTVPNLLKNLSPEEEKPQPPSVPGLTHPLLEVPSQNWPWESECEQMEKEEPS
LSITEVPDSSGDRRQDIPCRAHPLSPETRPSLLWKSQELGGQQDHQDLALTSDELLKQT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC060703
ORF Size 5217 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC060703.1
RefSeq Size 5315 bp
RefSeq ORF 5219 bp
Locus ID 17776
Gene Summary Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a multi-protein complex in spermatid maturation. Regulates lipopolysaccharide-induced IL-12 synthesis in macrophages by forming a complex with TRAF6, resulting in the inhibition of TRAF6 NF-kappa-B activation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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