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BC033915 (BC063268) Mouse Tagged ORF Clone
CAT＃: MR211911
- TrueORF®
BC033915 (Myc-DDK-tagged) - Mouse cDNA sequence BC033915 (cDNA clone MGC:68087 IMAGE:4187769)
ORF Plasmid: tGFP
"BC063268" in other vectors (4)
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CNY 9,128.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5730525O22Rik; 9030204A07Rik; AI447252; AI846254; AI849445; mKIAA0999; Qsk; SIK-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211911 representing BC063268
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCTCGCATCGGCTACTACGAGATCGACCGCACCATCGGCAAGGGCAACTTCGCTGTGGTCAAGC GGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTATTAAAATCATAGATAAGAGCCAGCTGGATGAAGA AAACTTGAAGAAGATTTTCCGGGAGGTTCAGATAATGAAGATGCTTTGCCATCCACACATCATCAGACTC TACCAGGTCATGGAGACAGAGCGCATGATTTACCTGGTGACAGAATACGCTAGCGGAGGGGAGATATTTG ACCACTTGGTAGCCCATGGAAGAATGGCAGAGAAGGAAGCTCGACGGAAGTTCAAACAGATCGTCACAGC GGTGTATTTTTGTCACTGTCGGAATATCGTTCATCGTGATTTAAAAGCCGAAAACTTACTTCTGGACGCC AATCTGAATATCAAAATAGCAGACTTTGGCTTCAGCAACCTCTTCACTCCAGGGCAGCTGCTGAAGACGT GGTGTGGCAGCCCTCCCTATGCCGCCCCAGAGCTCTTCGAAGGGAAGGAATATGATGGGCCCAAAGTGGA CATATGGAGCCTTGGAGTTGTCCTCTATGTGCTGGTGTGTGGCGCCCTGCCGTTTGATGGGAGCACACTG CAGAATCTGCGGGCCCGCGTGCTGAGTGGCAAGTTCCGCATCCCGTTCTTTATGTCCACAGAGTGTGAGC ACTTGATCCGCCACATGCTGGTGTTAGATCCAAATAAGCGCCTCTCAATGGAACAGATCTGCAGGCACAA GTGGATGAAGCTCGGAGATGCAGACCCCAACTTTGACAGGTTAATAGCGGAGTGCCAGCAACTGAAGGAG GAAAGGCAGTCGGACCCCCTCAACGATGATGTCCTCTTGGCTATGGAAGACATGGGGCTGGACAAGGAGC GCACACTTCAGTCATTAAGGTCAGATGCCTATGACCACTATAGTGCAATCTACAGCCTGCTGTGCGATCG ACACAAGAAACATAAAACTCTGCGTCCCGGAGCACTTCCCAGCATGCCCCAAGCCATGACTTTCCAGGCA CCAGTCAATCTCCAGGCGGAGCAGACAGGCACTGCTATGAACCTTAGCGTCCCTCAAGTTCAGCTGATCA ACCCAGAGAACCAAATTATAGAGCCTGACGGGGCTGTGAACTTGGACAGTGATGAGGGTGAAGAGCCTTC TCCAGAAGCCTTGGTTCGCTATTTGTCAATGAGGAGGCACACGGTGGGAGTGGCTGACCCACGCACGGAA GTTATGGAAGATCTGCAGAAGCTGCTGCCTGGCTTTCCTGGAGTAAACCCACAGGGTCCATTCCTACAAG TGGCCCCTAACATGAACTTCACGCACAACCTGTTGCCCATGCAGAGTCTGCAGCCAACTGGGCAGCTTGA GTACAAGGAACAGTCTCTGTTACAGCCGCCTACACTACAGCTACTGAACGGAATGGGGCCCCTTGGCCGG AGAGCCTCAGATGGAGGCGCCAACATCCAACTGCATGCCCAGCAGCTGCTCAAGCGCCCACGGGGACCGT CCCCACTTGTCACCATGACACCAGCAGTGCCAGCAGTTACCCCTGTGGATGAGGAGAGCTCGGATGGGGA GCCAGATCAGGAAGCTGTGCAGAGGTACTTGGCAAATAGGTCCAAAAGGCACACACTGGCCATGACCAGC CCGACAGCTGAGATCCCACCGGACCTGCAACGGCAGCTAGGACAACAGTCTTTCCGTTCCCGGGTCTGGC CTCCTCACCTGGTACCTGACCAGCATCGCTCCACCTACAAGGACTCCAACACCCTGCATCTCCCTACGGA GCGTTTCTCCCCCGTGCGCCGGTTCTCAGACGGGGCTGCAAGCATCCAGGCCTTCAAAGCTCACCTGGAA AAAATGGGCAACAGCAGCAGCATCAAGCAGTTGCAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGG GGCAGGTGGATGAGAGGACCCTGGAGAAGACCCAGCAGCAGCATATGTTGTACCAGCAGGAGCAGCACCA TCAGATCCTCCAGCAGCAAATCCAAGATTCTATTTGTCCTCCTCAGCCCTCCCCACCTCTTCAGGTTGCC TGTGAAAACCAACCAGCCCTCCTCACCCACCAGCTCCAGAGGTTAAGGATTCAACCTTCAAGCCCACCCC CCAACCATCCCAGCAACCATCTCTTCAGACAGCCAAGTAACAGTCCTCCCCCGGTCAGCAGTGCCATGAT CACGTCTCACGGTGCTACGTCTCCCTCCCAGTTTCAAGGCTTACCCTCCCATGGTGCAATCTTCCAGCAG CAACCCGAGAACTGTTCCCCGCCTCCCAGTGTAGCACTAACCTGCCTGGGCCTACAGCAGGCTAGCCAGT CACAGCCAGTGACTATCCAGCTACAGGAGCCAGTTGACATGCTCAGCAACATGGCCGGAACCGCGGCAGG CTCTGCAGGGCGGAGCATCCCCATCAGCCCCAGCGCCAGTCAGATTCAGATACAGCACCGAGCCAGCCTA ATGGCTCCCTTCAGCTATGGGCACCGGCCCTTGTCCAAGCAGCTGAGCGCTGACAGTGCAGAGGCCCACA GCTTGAACATGAATCGGTTCTCCCCTGCCAACTACGACCAGGCGCATTTACACCCCCATCTGTTTTCGGA CCAGTCCCGAGGTTCCCCCAGCAGCTACAGCCCTTCAACAGGAGTGGGGTTTCCTCCAACTCAAGCCCTG AAAGTTCCTCCGCTTGACCAGTTCCCCACTTTCCCTCCCAGTGCCCAGCAGCAGCCACCCCACTATACCA CGTCAGCACTACAGCAGGCCCTGCTATCTCCTACACCGCCAGACTATCCCCGACACCAGCAGGTTCCCCA TATCCTTCAAGGACTGCTCTCTCCCCGGCATTCACTCACCGGCCACTCGGACATTCGGCTGCCTCCGGCA GAGTTTGCACAGCTCATCAAAAGGCAGCAACAGCATCGACAGCAGCAGCAGCAGCAGCAGCAGCAACAAG AATACCATGAATTGTTCAGGCACATGAACCAAGGGGATGCTGTTAGCCTAGCTCCCAGCCTCGGGGGACA GAATATGACAGAGCACCAGGCTTTATCTTATCAAAATGCTGACTCGTACCACCGCCACCACACCAGCCCC CAGCATATCTTACAGATCAGGGCGCAAGATTGTATCTCACAGGGTCCCTCGCCCACCCCCACCCATGGCT ATGCCCATCAGCCACCACTAATGCATTCGGAGAGTATGGAGGAAGACTGCTTGTGTGAGGGGCTCAAGGA GGGCTTCCCAGACAAGAGCTCAAGCACACTGACCAAAGGTTGCCACAACAGCCCTCTGCTCTTGTGTACC AGTGGGCCTGGGGACCCTGAGCCTTTGCTGGGAACTGTGAGTCAGGCCCGGGAGCTGGGGATCCATCCCT ACGGACACCAGCCAACTGCCACCACGTTCAGTAGAAATAAGGTGCCCAGCCGAGAGTCTGTCCTAGGGAA CTGCCTGGAAAGAAGTTCTCCTGGACAAGCAATGGAGCTGCCGGATCACAACGGCCTTGGGTACCCAGTA CGGCCCTTAGTCAGTGAGCACCTCAGGTCCCGGACGCTCCAGAGACACCACACGATCCAGAACAGCGACG ATGCCTATGTACAGCTGGATACCTTGCCAGGAATGAGCCTGGTGGCAGGCAAGGCGCTGAGCTCTGCCCG GATGTCAGATGCAGTTCTTAGTCAGTCTTCACTCATGGGCAGCCAACAGTTTCAGGATGAGGAAGATGAA GAATGTGGGGTGAGCCTGGGCCACGAGCATCCAGGCCTGGGTGATGGCAACCAGCATCTCAACTCCTCTC GCTATCCAGCTACGTGTGTTACAGACATCATGCTCAGCCACAAGCACCCAGAGGTCTCCTTCAGCATGGA GCAAGCCGGCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211911 representing BC063268
Red=Cloning site Green=Tags(s) MAARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKSQLDEENLKKIFREVQIMKMLCHPHIIRL YQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDA NLNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTL QNLRARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICRHKWMKLGDADPNFDRLIAECQQLKE ERQSDPLNDDVLLAMEDMGLDKERTLQSLRSDAYDHYSAIYSLLCDRHKKHKTLRPGALPSMPQAMTFQA PVNLQAEQTGTAMNLSVPQVQLINPENQIIEPDGAVNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTE VMEDLQKLLPGFPGVNPQGPFLQVAPNMNFTHNLLPMQSLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGR RASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQEAVQRYLANRSKRHTLAMTS PTAEIPPDLQRQLGQQSFRSRVWPPHLVPDQHRSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLE KMGNSSSIKQLQQECEQLQKMYGGQVDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQVA CENQPALLTHQLQRLRIQPSSPPPNHPSNHLFRQPSNSPPPVSSAMITSHGATSPSQFQGLPSHGAIFQQ QPENCSPPPSVALTCLGLQQASQSQPVTIQLQEPVDMLSNMAGTAAGSAGRSIPISPSASQIQIQHRASL MAPFSYGHRPLSKQLSADSAEAHSLNMNRFSPANYDQAHLHPHLFSDQSRGSPSSYSPSTGVGFPPTQAL KVPPLDQFPTFPPSAQQQPPHYTTSALQQALLSPTPPDYPRHQQVPHILQGLLSPRHSLTGHSDIRLPPA EFAQLIKRQQQHRQQQQQQQQQQEYHELFRHMNQGDAVSLAPSLGGQNMTEHQALSYQNADSYHRHHTSP QHILQIRAQDCISQGPSPTPTHGYAHQPPLMHSESMEEDCLCEGLKEGFPDKSSSTLTKGCHNSPLLLCT SGPGDPEPLLGTVSQARELGIHPYGHQPTATTFSRNKVPSRESVLGNCLERSSPGQAMELPDHNGLGYPV RPLVSEHLRSRTLQRHHTIQNSDDAYVQLDTLPGMSLVAGKALSSARMSDAVLSQSSLMGSQQFQDEEDE ECGVSLGHEHPGLGDGNQHLNSSRYPATCVTDIMLSHKHPEVSFSMEQAGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC063268 |
| ORF Size | 3933 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC063268.1 |
| RefSeq Size | 6099 bp |
| RefSeq ORF | 3935 bp |
| Locus ID | 70661 |
| MW | 223.6 kDa |
| Gene Summary | Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor (By similarity). Required for chondrocyte hypertrophy during skeletogenesis (PubMed:22318228). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (By similarity). Prevents HDAC4 translocation to the nucleus (PubMed:22318228).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223095 | BC033915 (untagged) - Mouse cDNA sequence BC033915 (cDNA clone MGC:68087 IMAGE:4187769), (10ug) |
CNY 12,920.00 |
|
| MG211911 | BC033915 (tGFP-tagged) - Mouse cDNA sequence BC033915 (cDNA clone MGC:68087 IMAGE:4187769) |
CNY 8,270.00 |
|
| MR211911L3 | Lenti ORF clone of BC033915 (Myc-DDK-tagged) - Mouse cDNA sequence BC033915 (cDNA clone MGC:68087 IMAGE:4187769) |
CNY 9,410.00 |
|
| MR211911L4 | Lenti ORF clone of BC033915 (mGFP-tagged) - Mouse cDNA sequence BC033915 (cDNA clone MGC:68087 IMAGE:4187769) |
CNY 9,410.00 |
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