BC033915 (BC063268) Mouse Tagged ORF Clone

CAT#: MG211911

  • TrueORF®

BC033915 (tGFP-tagged) - Mouse cDNA sequence BC033915 (cDNA clone MGC:68087 IMAGE:4187769)

ORF Plasmid: DDK tGFP



  "BC063268" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 8,270.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 5730525O22Rik; 9030204A07Rik; AI447252; AI846254; AI849445; mKIAA0999; Qsk; SIK-3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211911 representing BC063268
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCTCGCATCGGCTACTACGAGATCGACCGCACCATCGGCAAGGGCAACTTCGCTGTGGTCAAGC
GGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTATTAAAATCATAGATAAGAGCCAGCTGGATGAAGA
AAACTTGAAGAAGATTTTCCGGGAGGTTCAGATAATGAAGATGCTTTGCCATCCACACATCATCAGACTC
TACCAGGTCATGGAGACAGAGCGCATGATTTACCTGGTGACAGAATACGCTAGCGGAGGGGAGATATTTG
ACCACTTGGTAGCCCATGGAAGAATGGCAGAGAAGGAAGCTCGACGGAAGTTCAAACAGATCGTCACAGC
GGTGTATTTTTGTCACTGTCGGAATATCGTTCATCGTGATTTAAAAGCCGAAAACTTACTTCTGGACGCC
AATCTGAATATCAAAATAGCAGACTTTGGCTTCAGCAACCTCTTCACTCCAGGGCAGCTGCTGAAGACGT
GGTGTGGCAGCCCTCCCTATGCCGCCCCAGAGCTCTTCGAAGGGAAGGAATATGATGGGCCCAAAGTGGA
CATATGGAGCCTTGGAGTTGTCCTCTATGTGCTGGTGTGTGGCGCCCTGCCGTTTGATGGGAGCACACTG
CAGAATCTGCGGGCCCGCGTGCTGAGTGGCAAGTTCCGCATCCCGTTCTTTATGTCCACAGAGTGTGAGC
ACTTGATCCGCCACATGCTGGTGTTAGATCCAAATAAGCGCCTCTCAATGGAACAGATCTGCAGGCACAA
GTGGATGAAGCTCGGAGATGCAGACCCCAACTTTGACAGGTTAATAGCGGAGTGCCAGCAACTGAAGGAG
GAAAGGCAGTCGGACCCCCTCAACGATGATGTCCTCTTGGCTATGGAAGACATGGGGCTGGACAAGGAGC
GCACACTTCAGTCATTAAGGTCAGATGCCTATGACCACTATAGTGCAATCTACAGCCTGCTGTGCGATCG
ACACAAGAAACATAAAACTCTGCGTCCCGGAGCACTTCCCAGCATGCCCCAAGCCATGACTTTCCAGGCA
CCAGTCAATCTCCAGGCGGAGCAGACAGGCACTGCTATGAACCTTAGCGTCCCTCAAGTTCAGCTGATCA
ACCCAGAGAACCAAATTATAGAGCCTGACGGGGCTGTGAACTTGGACAGTGATGAGGGTGAAGAGCCTTC
TCCAGAAGCCTTGGTTCGCTATTTGTCAATGAGGAGGCACACGGTGGGAGTGGCTGACCCACGCACGGAA
GTTATGGAAGATCTGCAGAAGCTGCTGCCTGGCTTTCCTGGAGTAAACCCACAGGGTCCATTCCTACAAG
TGGCCCCTAACATGAACTTCACGCACAACCTGTTGCCCATGCAGAGTCTGCAGCCAACTGGGCAGCTTGA
GTACAAGGAACAGTCTCTGTTACAGCCGCCTACACTACAGCTACTGAACGGAATGGGGCCCCTTGGCCGG
AGAGCCTCAGATGGAGGCGCCAACATCCAACTGCATGCCCAGCAGCTGCTCAAGCGCCCACGGGGACCGT
CCCCACTTGTCACCATGACACCAGCAGTGCCAGCAGTTACCCCTGTGGATGAGGAGAGCTCGGATGGGGA
GCCAGATCAGGAAGCTGTGCAGAGGTACTTGGCAAATAGGTCCAAAAGGCACACACTGGCCATGACCAGC
CCGACAGCTGAGATCCCACCGGACCTGCAACGGCAGCTAGGACAACAGTCTTTCCGTTCCCGGGTCTGGC
CTCCTCACCTGGTACCTGACCAGCATCGCTCCACCTACAAGGACTCCAACACCCTGCATCTCCCTACGGA
GCGTTTCTCCCCCGTGCGCCGGTTCTCAGACGGGGCTGCAAGCATCCAGGCCTTCAAAGCTCACCTGGAA
AAAATGGGCAACAGCAGCAGCATCAAGCAGTTGCAGCAGGAGTGTGAGCAGCTGCAGAAGATGTACGGGG
GGCAGGTGGATGAGAGGACCCTGGAGAAGACCCAGCAGCAGCATATGTTGTACCAGCAGGAGCAGCACCA
TCAGATCCTCCAGCAGCAAATCCAAGATTCTATTTGTCCTCCTCAGCCCTCCCCACCTCTTCAGGTTGCC
TGTGAAAACCAACCAGCCCTCCTCACCCACCAGCTCCAGAGGTTAAGGATTCAACCTTCAAGCCCACCCC
CCAACCATCCCAGCAACCATCTCTTCAGACAGCCAAGTAACAGTCCTCCCCCGGTCAGCAGTGCCATGAT
CACGTCTCACGGTGCTACGTCTCCCTCCCAGTTTCAAGGCTTACCCTCCCATGGTGCAATCTTCCAGCAG
CAACCCGAGAACTGTTCCCCGCCTCCCAGTGTAGCACTAACCTGCCTGGGCCTACAGCAGGCTAGCCAGT
CACAGCCAGTGACTATCCAGCTACAGGAGCCAGTTGACATGCTCAGCAACATGGCCGGAACCGCGGCAGG
CTCTGCAGGGCGGAGCATCCCCATCAGCCCCAGCGCCAGTCAGATTCAGATACAGCACCGAGCCAGCCTA
ATGGCTCCCTTCAGCTATGGGCACCGGCCCTTGTCCAAGCAGCTGAGCGCTGACAGTGCAGAGGCCCACA
GCTTGAACATGAATCGGTTCTCCCCTGCCAACTACGACCAGGCGCATTTACACCCCCATCTGTTTTCGGA
CCAGTCCCGAGGTTCCCCCAGCAGCTACAGCCCTTCAACAGGAGTGGGGTTTCCTCCAACTCAAGCCCTG
AAAGTTCCTCCGCTTGACCAGTTCCCCACTTTCCCTCCCAGTGCCCAGCAGCAGCCACCCCACTATACCA
CGTCAGCACTACAGCAGGCCCTGCTATCTCCTACACCGCCAGACTATCCCCGACACCAGCAGGTTCCCCA
TATCCTTCAAGGACTGCTCTCTCCCCGGCATTCACTCACCGGCCACTCGGACATTCGGCTGCCTCCGGCA
GAGTTTGCACAGCTCATCAAAAGGCAGCAACAGCATCGACAGCAGCAGCAGCAGCAGCAGCAGCAACAAG
AATACCATGAATTGTTCAGGCACATGAACCAAGGGGATGCTGTTAGCCTAGCTCCCAGCCTCGGGGGACA
GAATATGACAGAGCACCAGGCTTTATCTTATCAAAATGCTGACTCGTACCACCGCCACCACACCAGCCCC
CAGCATATCTTACAGATCAGGGCGCAAGATTGTATCTCACAGGGTCCCTCGCCCACCCCCACCCATGGCT
ATGCCCATCAGCCACCACTAATGCATTCGGAGAGTATGGAGGAAGACTGCTTGTGTGAGGGGCTCAAGGA
GGGCTTCCCAGACAAGAGCTCAAGCACACTGACCAAAGGTTGCCACAACAGCCCTCTGCTCTTGTGTACC
AGTGGGCCTGGGGACCCTGAGCCTTTGCTGGGAACTGTGAGTCAGGCCCGGGAGCTGGGGATCCATCCCT
ACGGACACCAGCCAACTGCCACCACGTTCAGTAGAAATAAGGTGCCCAGCCGAGAGTCTGTCCTAGGGAA
CTGCCTGGAAAGAAGTTCTCCTGGACAAGCAATGGAGCTGCCGGATCACAACGGCCTTGGGTACCCAGTA
CGGCCCTTAGTCAGTGAGCACCTCAGGTCCCGGACGCTCCAGAGACACCACACGATCCAGAACAGCGACG
ATGCCTATGTACAGCTGGATACCTTGCCAGGAATGAGCCTGGTGGCAGGCAAGGCGCTGAGCTCTGCCCG
GATGTCAGATGCAGTTCTTAGTCAGTCTTCACTCATGGGCAGCCAACAGTTTCAGGATGAGGAAGATGAA
GAATGTGGGGTGAGCCTGGGCCACGAGCATCCAGGCCTGGGTGATGGCAACCAGCATCTCAACTCCTCTC
GCTATCCAGCTACGTGTGTTACAGACATCATGCTCAGCCACAAGCACCCAGAGGTCTCCTTCAGCATGGA
GCAAGCCGGCGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211911 representing BC063268
Red=Cloning site Green=Tags(s)

MAARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKSQLDEENLKKIFREVQIMKMLCHPHIIRL
YQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDA
NLNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTL
QNLRARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICRHKWMKLGDADPNFDRLIAECQQLKE
ERQSDPLNDDVLLAMEDMGLDKERTLQSLRSDAYDHYSAIYSLLCDRHKKHKTLRPGALPSMPQAMTFQA
PVNLQAEQTGTAMNLSVPQVQLINPENQIIEPDGAVNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTE
VMEDLQKLLPGFPGVNPQGPFLQVAPNMNFTHNLLPMQSLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGR
RASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQEAVQRYLANRSKRHTLAMTS
PTAEIPPDLQRQLGQQSFRSRVWPPHLVPDQHRSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLE
KMGNSSSIKQLQQECEQLQKMYGGQVDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQVA
CENQPALLTHQLQRLRIQPSSPPPNHPSNHLFRQPSNSPPPVSSAMITSHGATSPSQFQGLPSHGAIFQQ
QPENCSPPPSVALTCLGLQQASQSQPVTIQLQEPVDMLSNMAGTAAGSAGRSIPISPSASQIQIQHRASL
MAPFSYGHRPLSKQLSADSAEAHSLNMNRFSPANYDQAHLHPHLFSDQSRGSPSSYSPSTGVGFPPTQAL
KVPPLDQFPTFPPSAQQQPPHYTTSALQQALLSPTPPDYPRHQQVPHILQGLLSPRHSLTGHSDIRLPPA
EFAQLIKRQQQHRQQQQQQQQQQEYHELFRHMNQGDAVSLAPSLGGQNMTEHQALSYQNADSYHRHHTSP
QHILQIRAQDCISQGPSPTPTHGYAHQPPLMHSESMEEDCLCEGLKEGFPDKSSSTLTKGCHNSPLLLCT
SGPGDPEPLLGTVSQARELGIHPYGHQPTATTFSRNKVPSRESVLGNCLERSSPGQAMELPDHNGLGYPV
RPLVSEHLRSRTLQRHHTIQNSDDAYVQLDTLPGMSLVAGKALSSARMSDAVLSQSSLMGSQQFQDEEDE
ECGVSLGHEHPGLGDGNQHLNSSRYPATCVTDIMLSHKHPEVSFSMEQAGV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC063268
ORF Size 3933 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC063268.1
RefSeq Size 6099 bp
RefSeq ORF 3935 bp
Locus ID 70661
Gene Summary Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor (By similarity). Required for chondrocyte hypertrophy during skeletogenesis (PubMed:22318228). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (By similarity). Prevents HDAC4 translocation to the nucleus (PubMed:22318228).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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