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Helb (NM_080446) Mouse Tagged Lenti ORF Clone
CAT＃: MR211593L3
- LentiORF®
Lenti ORF clone of Helb (Myc-DDK-tagged) - Mouse helicase (DNA) B (Helb)
"NM_080446" in other vectors (4)
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CNY 11,310.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI447783; D10Ertd664e |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR211593).
|
| Restriction Sites | SgfI-RsrII Cloning Scheme for this gene |
| ACCN | NM_080446 |
| ORF Size | 3222 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080446.2, NP_536694.2 |
| RefSeq Size | 4540 bp |
| RefSeq ORF | 3225 bp |
| Locus ID | 117599 |
| UniProt ID | Q6NVF4 |
| Gene Summary | 5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection (PubMed:26774285). Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB (PubMed:26774285). As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation (PubMed:26774285). Acts independently of TP53BP1 (PubMed:26774285). Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates. During S phase, may facilitate cellular recovery from replication stress (PubMed:11557815, PubMed:7596831, PubMed:7794903).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203460 | Helb (untagged) - Mouse helicase (DNA) B (Helb), (10ug) |
CNY 7,488.00 |
|
| MG211593 | Helb (tGFP-tagged) - Mouse helicase (DNA) B (Helb) |
CNY 10,360.00 |
|
| MR211593 | Helb (Myc-DDK-tagged) - Mouse helicase (DNA) B (Helb) |
CNY 7,480.00 |
|
| MR211593L4 | Lenti ORF clone of Helb (mGFP-tagged) - Mouse helicase (DNA) B (Helb) |
CNY 11,310.00 |
