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Helb (NM_080446) Mouse Tagged ORF Clone
CAT＃: MG211593
- TrueORF®
Helb (tGFP-tagged) - Mouse helicase (DNA) B (Helb)
ORF Plasmid: DDK
"NM_080446" in other vectors (4)
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CNY 10,360.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI447783; D10Ertd664e |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211593 representing NM_080446
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAGGCAGGATCGTCTGCGCGAGCTCCTGGGCCCTCTGCACCCGTACAAGAGCGACGACGAGGAGG AGGATTGTGCGCAGGAGGAGGAGGGGGAGCAGGAGGAGGAGTTTGTGGATGCCGAGGAACTCTGCAGCGG GGGCATCAAGGCGGGCAGCCTCCCTGGGCGCGCGCGAGTGTCTATCCCTGATGAGTATACTAAAGAGAAA TGTACCGTGTACGGACGTTTCCCACTCAAAGGTCCGTGGTGGCGCGTGAAGGTCCAGGTCCTGAAACCCC AGCGATCCAGGAGCTATCAAGTTCAAGGCTTTCCGGCATACTTTCTACAGGTTGATATGTCGCCACCAGA TCAAAAACAAATCTGTTCGCTTTTTCTTAAAGAATGCAACTTAGCCTCTGAGCGCATACAGGAATTTTTA AAATGGGTAGAAAAAGTGTCAAGCTTTGAAAATCTACACTTTGAAAATCTTTGGGAAACATTAAGGCTTT TCTACAGAGAAACCGAAAAGAAAGATAAAAAGCTGTCTACACCAAGGGAGCAGCAGGGTGAGGAGATGAG AGTGGAAAAATCATTCGCATTTATAAGTGCAATGGTAGCTCTGCAATTCCCCAAAGTCATGGAATTCCTT CCATCGCTTTTCCCTCGACACTTCAAAAGGCTCATCAGCTCGAGTTCTGATTGGGTGCTGGGATGCATAG AAGATGTTCTGGGTACACAGCCGTGGAAGCTGGGCTTCAGGAGGATAACCTACAGAGAAATGAAACTAGT GCGCTGTGAGGCCAGCTGGACAGCGTTCAGTCAGTGCCCCTCGCTGCTCCAGCTGATGACGCCCCTGCAG AAGAATGCATTGGTCATATATTCTAAGCTGAGGCAGACATGCAGAGAAGACGGACACACGTACATTGAAG TAAAAGACTTGACCTCGGGCTTGTCGGAGCATATGTCATTCGAGGAAGCGTGTCAGTCTCTGGCGTTTCT GAAGGACATTGACGTGGTCATTTATGAGAAGGACTATGTCTTTCTTTCTGAACTGTACGAGGCCGAGCAA GACATCGCCTCCTCCATCTGCGAGCTGATGAGCAGACCTCCGTGGCACTTAAAAGTGGACGTGAAGAACG TCCTGGCGTCAATTAGAGGCGCAAAGCCCAACGATCCAGGAAGTGCTGAGGCAGTGGAAGGGAGCAAGCC GGAGGAGGTGGGGTCAGAGCAGGGTGACAGTGTTTTAGACGCCCAAGATGGCGATGACCATGTTAGAAGC AATGGCGAACACGTCGCAAATGCAGAAATAAACGACGTCCCACTGGATCAAGATCAGGTGGTCGCCCTGG AGACGATCTGCGCCAACGCGGTGACTGTCCTCAGTGGGAAAGGCGGCTGTGGGAAGACCACCATTGTTAG CCGTCTTTTTAAGCACATGGAACATCTGGAAGAAACGGAAGTGCAGCAAGCCTGCGAGGATTTTGAACAA GACCAGGAAGCCTCAGAAGAGTGGCTTGACTGTCCTAAGCAGAGCCCAGCCGGTGTGGACAAGGCTGTAG AAGTCTTGCTCACCGCACCTACAGGGAAGGCTGCTGGCTTATTGAGGCAGAGGACCGATCTTCCGGCTTA CACGCTGTGCCAGGTCAATTACAGCTTCTATATGTGGAAAACGAAAAATGAGGTGGACAAGCCGTGGAAA TTCTCCACGGTTCGAGTTCTGGTTGTGGACGAAGGGAGTTTGGTGTCTGTAGGGATCTTCAAGTCAGTGT TACAGTTGCTGTGCAAACACTCCAAGCTGTCCAAGCTCATCATCCTTGGTGATGTTAGACAGTTGCCCAG TATTGAACCTGGTAACATGCTGCAGGATGTCTTTGAGACTCTTAAGTCAAGACAGTGTGCCATCGAACTA AAGACGAACCACAGGACAGAGTCTCAGCTGATTGTGGACAATGCTACAAGAATCTCCAGACGTCAGTTTC CAAAATTTGATGCTGAACTAAATATCTGTGGTAATCCTACATTACCTCTCTCCATTCAAGATAAGACATT TATCTTTGTGAGACTCCCTGAAGAAGACAGCAGATCTCAGTCGTCTAAAGGTGAACACCGATCTAATTTA TATACTGCAGTTAAAACTTTGCTTCAAGGAAAGGACTTCTGCTCTTTTGAAAGTTCAAAAACATCGCAAT TTATTGCATTTAGAAGGCAAGACTGTGACCTCATCAATGACTGCTGCTGCAAGCACTACACAGGCCACCT CATCAAAGACCATGAGAAGAAACTCATCTTTGCCGTCGGTGATAAGATCTGCTGTACCCGGAATGCATAC CTCTCAGATTTACTCCCCGACAAAGACCAGGAAGCCGAGGGGAAAGGCTATGGAGACGCCCCTGACGACG ATGCTAAAATCAAACAGGATTTCGAAAGCAGCACCCGCCTGTGCAACGGGGAAATATTTTTCATAACACG GGATGTAACTGACGTAACTTTCAAAAGAAAAAGACTTCTGACCATTAATAACGAGGCCGGTTTGGAAGTG ACTGTGGACTTTAGCAAACTAATGGCAAACTGTCAGATCAAGCATGCCTGGGCAAGGACCATTCATACTT TTCAGGGGTCCGAGGAGAACACAGTCGTCTACGTCGTGGGGAAGGCAGGCCGCCAGCACTGGCAACATGT TTACACGGCAGTGACCCGGGGCCGCTCCAGAGTGTACATCATCGCGCAGGAGTCTGAACTCAGGAGTGCC ACCCGGAAGCGCGGTTTCCCCAGGCAGACGCGTTTGAAACACTTCTTGCAGAAGAAGCTCTCTGGCAGCT GCGCACCCTCAACAGGTTTCGCATCCCAGCCTTCGAGCCCTCGAGTCGGAGGAAGACCTGACACCCAGCC TCCAGCGTCACACCTCTGTAGAACCCCAGACAACAAAGCCACTGCAGACAGCGCCAGAGGCGACGAAAGA TGGCTGTCAGCTTCTGTTAACGATGACGTGGATACGGATGAGGAGTCAGCACAGCTCAGAGGGTCCAAAA GAATCGGTGACGGCTTCCCGTTTGATGAAGAAAGCCCCAGTAAATTCCGTATGGTTGAAGCGCCATCTCC ACAGGTGTCCTCTGTGTTTCAGAATATGAGGTTGAATACTCTCACTCCCAGACAGCTTTTTAAACCCACC GACAATCAAGACACAGGTACGGCAGGTGTGGCGGACGACGCCAACGATCCCAGCAATCAGGAGATGGAAA TG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211593 representing NM_080446
Red=Cloning site Green=Tags(s) MARQDRLRELLGPLHPYKSDDEEEDCAQEEEGEQEEEFVDAEELCSGGIKAGSLPGRARVSIPDEYTKEK CTVYGRFPLKGPWWRVKVQVLKPQRSRSYQVQGFPAYFLQVDMSPPDQKQICSLFLKECNLASERIQEFL KWVEKVSSFENLHFENLWETLRLFYRETEKKDKKLSTPREQQGEEMRVEKSFAFISAMVALQFPKVMEFL PSLFPRHFKRLISSSSDWVLGCIEDVLGTQPWKLGFRRITYREMKLVRCEASWTAFSQCPSLLQLMTPLQ KNALVIYSKLRQTCREDGHTYIEVKDLTSGLSEHMSFEEACQSLAFLKDIDVVIYEKDYVFLSELYEAEQ DIASSICELMSRPPWHLKVDVKNVLASIRGAKPNDPGSAEAVEGSKPEEVGSEQGDSVLDAQDGDDHVRS NGEHVANAEINDVPLDQDQVVALETICANAVTVLSGKGGCGKTTIVSRLFKHMEHLEETEVQQACEDFEQ DQEASEEWLDCPKQSPAGVDKAVEVLLTAPTGKAAGLLRQRTDLPAYTLCQVNYSFYMWKTKNEVDKPWK FSTVRVLVVDEGSLVSVGIFKSVLQLLCKHSKLSKLIILGDVRQLPSIEPGNMLQDVFETLKSRQCAIEL KTNHRTESQLIVDNATRISRRQFPKFDAELNICGNPTLPLSIQDKTFIFVRLPEEDSRSQSSKGEHRSNL YTAVKTLLQGKDFCSFESSKTSQFIAFRRQDCDLINDCCCKHYTGHLIKDHEKKLIFAVGDKICCTRNAY LSDLLPDKDQEAEGKGYGDAPDDDAKIKQDFESSTRLCNGEIFFITRDVTDVTFKRKRLLTINNEAGLEV TVDFSKLMANCQIKHAWARTIHTFQGSEENTVVYVVGKAGRQHWQHVYTAVTRGRSRVYIIAQESELRSA TRKRGFPRQTRLKHFLQKKLSGSCAPSTGFASQPSSPRVGGRPDTQPPASHLCRTPDNKATADSARGDER WLSASVNDDVDTDEESAQLRGSKRIGDGFPFDEESPSKFRMVEAPSPQVSSVFQNMRLNTLTPRQLFKPT DNQDTGTAGVADDANDPSNQEMEM SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080446 |
| ORF Size | 3222 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080446.2, NP_536694.2 |
| RefSeq Size | 4540 bp |
| RefSeq ORF | 3225 bp |
| Locus ID | 117599 |
| UniProt ID | Q6NVF4 |
| Gene Summary | 5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection (PubMed:26774285). Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB (PubMed:26774285). As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation (PubMed:26774285). Acts independently of TP53BP1 (PubMed:26774285). Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates. During S phase, may facilitate cellular recovery from replication stress (PubMed:11557815, PubMed:7596831, PubMed:7794903).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203460 | Helb (untagged) - Mouse helicase (DNA) B (Helb), (10ug) |
CNY 7,488.00 |
|
| MR211593 | Helb (Myc-DDK-tagged) - Mouse helicase (DNA) B (Helb) |
CNY 7,480.00 |
|
| MR211593L3 | Lenti ORF clone of Helb (Myc-DDK-tagged) - Mouse helicase (DNA) B (Helb) |
CNY 11,310.00 |
|
| MR211593L4 | Lenti ORF clone of Helb (mGFP-tagged) - Mouse helicase (DNA) B (Helb) |
CNY 11,310.00 |
