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Ube2o (BC059193) Mouse Tagged Lenti ORF Clone
CAT＃: MR211566L4
- LentiORF®
Lenti ORF clone of Ube2o (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2O (cDNA clone MGC:65407 IMAGE:4160667)
"BC059193" in other vectors (4)
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CNY 12,540.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | E2-230K, mKIAA1734, 9630022H21 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR211566).
|
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | BC059193 |
| ORF Size | 3186 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC059193.1 |
| RefSeq Size | 4569 bp |
| RefSeq ORF | 3188 bp |
| Locus ID | 217342 |
| Gene Summary | E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223123 | Ube2o (untagged) - Mouse ubiquitin-conjugating enzyme E2O (cDNA clone MGC:65407 IMAGE:4160667), (10ug) |
CNY 12,920.00 |
|
| MG211566 | Ube2o (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2O (cDNA clone MGC:65407 IMAGE:4160667) |
CNY 11,690.00 |
|
| MR211566 | Ube2o (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2O (cDNA clone MGC:65407 IMAGE:4160667) |
CNY 10,640.00 |
|
| MR211566L3 | Lenti ORF clone of Ube2o (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2O (cDNA clone MGC:65407 IMAGE:4160667) |
CNY 12,540.00 |
