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Ube2o (BC059193) Mouse Tagged ORF Clone
CAT＃: MR211566
- TrueORF®
Ube2o (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2O (cDNA clone MGC:65407 IMAGE:4160667)
ORF Plasmid: tGFP
"BC059193" in other vectors (4)
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CNY 10,640.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E2-230K, mKIAA1734, 9630022H21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211566 representing BC059193
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACACAGAAGATGGTGCCAAACTCTACGATGTCTGCCCTCACGTCAGCGACTCGGGTCTCTTCTTTG ATGACTCCTATGGCTTCTACCCAGGCCAAGTCCTTATTGGCCCAGCCAAGATCTTCTCCAGTGTCCAGTG GCTCTCGGGGGTCAAGCCTGTACTCAGCACCAAGAGCAAGTTCCGTGTGGTGGTGGAGGAGGTGCAGGTT GTAGAACTGAAAGTCACGTGGATTACCAAGAGCTTCTGCCCCGGCGGCACGGACAGCGTTAGCCCCCCAC CTTCCATCATCACTCAGGAAAACCTAGGCAGGGTGAAGCGTCTCGGATGCTTTGACCATGCTCAGCGGCA GCTTGGGGAACGCTGTCTGTATGTCTTCCCAGCCAAGGTAGAGCCGGCCAAGATTGCCTGGGAATGTCCA GAAAAAAACTGCGCCCAGGGGGAGGGCTCTATGGCCAAGAAGGTGAAGCGTCTGTTGAAGAAGCAAGTTG TGAGAATCATGTCCTGCACCCCAGATACCCAGTGTCCTAGGGACCATTCCATGGAAGACCCAGACAAGAA AGGGGAAGCCAGAGCAGGGAGTGAAATAGGGTCTGCTAGCCCGGAGGAGCAACCTGATGGATCAGCCAGC CCCGTGGAGATGCAGGATGAGGGCTCGGAGGAGCTTCAGGAGACATGCGAGCCACTGCCCCCCTTCCTCT TGAAGGAGGGTGGAGATGACGGGCTGCACTCGGCAGAGCAGGACGCAGATGATGAGGCGGCTGACGATAC AGATGACACCAGCTCTGTGACCTCCTCCGCCAGCTCTACTACCTCCTCCCAGAGTGGCAGTGGCACTGGT CGTAAAAAGAGCATTCCTTTGTCCATCAAAAACTTGAAGCGAAAACACAAGAGGAAGAAGAATAAGGTCA CCCGTGACTTCAAGCCTGGAGACAGGGTGGCAGTGGAGGTTGTGACCACGATGACCTCAGCTGATGTGAT GTGGCAAGATGGCTCTGTGGAATGCAACATCCGCTCCAATGACCTTTTCCCTGTGCACCACCTGGACAAC AATGAGTTCTGCCCTGGGGACTTCGTGGTGGACAAGCGAGTCCAGAGCTGCCCAGACCCTGCTGTCTATG GTGTGGTGCAGTCTGGGGATCATGTTGGCCGTACCTGCATGGTGAAGTGGTTCAAGCTGCGACCAAGTGG GGATGACGTGGAGCTCATTGGAGAAGAGGAGGACGTGAGCGTCTATGACATTGCTGACCACCCTGACTTT CGCTTCCGCACAACTGACATTGTCATCCGCATTGGTAACACGGAGGATGGGGCTCTACCTAAGGAGGATG AGCCCTCAGTGGGCCAAGTGGCCCGAGTGGATGTCAGCAGCAAAGTGGAAGTGGTGTGGGCTGACAACTC GAAAACCATCATCCTGCCACAGCACTTATACAACATCGAGTCTGAGATTGAGGAGTCTGACTACGACTCC GTGGAAGGCAGCTCCAGTGGGGCCTCCTCGGATGAGTGGGAGGACGACAGCGACAGCTGGGAGACAGACA ACGGGCTCGTGGACGATGAGCACCCCAAGATAGAGGAGCTGGCCGCCATCCTGCCTGCCGAGCAGCCCAC TGCCCCCGAGGAGGACAAGGGCGTGGTCATCAGTGAGGAGGCGGCCACGGCTGCCATCCAGGGCGCCGTG GCCATGGCTGCCCCCGTGGCAGGGCTGATGGAGAAAGCTGGGAAGGACGGGCCTCCCAAGAGTTTCCGGG AGCTGAAGGAGGCCATCAAGATCCTGGAGAGCCTCAAGAACATGACTGTGGAGCAGCTGCTCACAGGCTC GCCCACCTCGCCCACCGTGGAGCCTGAGAAGCCAACCCGAGAGAAGAAGTTTTTGGATGATATCAAGAAG CTACAGGAGAACCTCAAGAAGACCCTGGACAATGTGGCCATTGCTGAGGAGGAGAAGATGGAGGCAGTAC CCGACACAGAGCGCAAGGAGGAAAAGCCTGAGGTGCAGTCCCCAGTGAAAGCCGAGTGGCCCAGTGAGAC ACCTGTGCTCTGTCAGCAGTGCGGTGGCAGGCCTGGCGTCACCTTCACCAGCGCCAAGGGCGAGGTCTTC TCTGTGCTGGAGTTTGCACCCTCAAACCACTCTTTCAAGAAGATTGAGTTCCAGCCCCCGGAAGCCAAGA AGTTCTTCAGCACAGTGCGGAAGGAGATGGCTCTGCTGGCCACCTCGCTGCCCGATGGCATCATGGTCAA GACTTTTGAGGACAGAATGGACCTCTTCTCTGCACTGATCAAGGGCCCCACTCGGACTCCCTATGAAGAC GGCCTCTACCTATTTGACATCCAGCTTCCCAACATCTACCCTGCGGTGCCCCCCCACTTTTGCTACCTCT CCCAGTGCAGTGGCCGCCTGAACCCTAACCTGTATGACAATGGGAAAGTGTGTGTCAGCCTCCTAGGTAC CTGGATTGGAAAGGGGACAGAGAGGTGGACAAGCAAGTCTAGCCTTCTCCAGGTGCTCATCTCCATCCAA GGTCTGATCCTGGTGAATGAACCATACTACAATGAAGCTGGCTTTGACAGTGACCGGGGCCTGCAGGAAG GCTATGAGAACAGTCGCTGCTACAACGAGATGGCATTAATCCGTGTGGTGCAGTCCATGACCCAGTTGGT GCGACGACCTCCTGAGGTCTTCGAACAGGAGATTCGGCAGCACTTCAGTGTGGGTGGCTGGCGGCTGGTG AACCGAATTGAGTCCTGGCTGGAAACCCATGCCATGCAGGAGAGGGCCCAGGTGATGCCCAATGGGGCCC CTAAAGACAGCAGCTCTCTGGAGCCCATGGCTGCAGCTGAACTCTCAGACTCCGGCCGTGAAGAGCCTGA AGATGTTGGGATGGCCCCTGGGGAGGCGTCCCAGGGCTCAGACTCAGAGGGTGGAGCCCAGGGCCCTGCC TCAGCCAGCAGAGACCACACAGAGCAGACTGAGACAGCCCCTGATGCATCCGCACCTCCCAGCGTTAGAC CAAAGAGGAGGAGGAAGAGCTACCGCAGCTTCCTGCCTGAGAAGAGCGGTTACCCTGACATCGGCTTCCC CCTCTTCCCACTCTCCAAGGGTTTCATCAAGAGCATCCGGGGGGTCCTGACACAGTTCCGTGCTGCCCTG CTGGAGGCGGGCATGCCGGAAAGCACAGAGGACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211566 representing BC059193
Red=Cloning site Green=Tags(s) MNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQV VELKVTWITKSFCPGGTDSVSPPPSIITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECP EKNCAQGEGSMAKKVKRLLKKQVVRIMSCTPDTQCPRDHSMEDPDKKGEARAGSEIGSASPEEQPDGSAS PVEMQDEGSEELQETCEPLPPFLLKEGGDDGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTG RKKSIPLSIKNLKRKHKRKKNKVTRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDN NEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDF RFRTTDIVIRIGNTEDGALPKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDS VEGSSSGASSDEWEDDSDSWETDNGLVDDEHPKIEELAAILPAEQPTAPEEDKGVVISEEAATAAIQGAV AMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKK LQENLKKTLDNVAIAEEEKMEAVPDTERKEEKPEVQSPVKAEWPSETPVLCQQCGGRPGVTFTSAKGEVF SVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYED GLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQ GLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLV NRIESWLETHAMQERAQVMPNGAPKDSSSLEPMAAAELSDSGREEPEDVGMAPGEASQGSDSEGGAQGPA SASRDHTEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAAL LEAGMPESTEDK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC059193 |
| ORF Size | 3186 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC059193.1 |
| RefSeq Size | 4569 bp |
| RefSeq ORF | 3188 bp |
| Locus ID | 217342 |
| MW | 167.5 kDa |
| Gene Summary | E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins. Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity. Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis. Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location. Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223123 | Ube2o (untagged) - Mouse ubiquitin-conjugating enzyme E2O (cDNA clone MGC:65407 IMAGE:4160667), (10ug) |
CNY 12,920.00 |
|
| MG211566 | Ube2o (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2O (cDNA clone MGC:65407 IMAGE:4160667) |
CNY 11,690.00 |
|
| MR211566L3 | Lenti ORF clone of Ube2o (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2O (cDNA clone MGC:65407 IMAGE:4160667) |
CNY 12,540.00 |
|
| MR211566L4 | Lenti ORF clone of Ube2o (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2O (cDNA clone MGC:65407 IMAGE:4160667) |
CNY 12,540.00 |
