Stk36 (BC043103) Mouse Tagged ORF Clone

CAT＃: MR211549

TrueORF^®

Stk36 (Myc-DDK-tagged) - Mouse serine/threonine kinase 36 (fused homolog, Drosophila) (cDNA clone MGC:58023 IMAGE:6406485), complete

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"BC043103" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 7,336.00

货期*

现货

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	FU, MGC58023, B930045J24
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR211549 representing BC043103 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAAGTACCACGTTTTGGAGATGATTGGAGAAGGCTCTTTTGGGAGAGTGTATAAGGGCCGAAAAA AATACAGTGCTCAGGTGGTGGCCTTGAAGTTCATCCCCAAACTGGGGCGCTCAGAGAAAGAGCTGAGGAA TCTGCAACGAGAGATTGAAATCATGCGGGGTCTGTGGCATCCCAACATTGTGCATATGCTCGACAGCTTT GAGACTGACAAAGAGGTGGTGGTGGTGACAGACTACGCTGAAGGAGAACTCTTTCAGATTCTGGAAGATG ATGGAAAACTTCCTGAAGACCAGGTTCAGGCCATCGCTGCCCAGTTGGTGTCAGCTCTGTACTACCTGCA TTCCCACCGCATCCTACACCGCGACATGAAACCGCAGAACATTCTTCTCGCCAAGGGTGGTGGCATTAAA CTTTGTGACTTTGGATTCGCCCGAGCTATGAGCACCAACACCATGGTGCTGACGTCGATCAAAGGCACAC CGCTCTATATGTCTCCAGAGCTGGTGGAGGAGCGACCATATGACCACACCGCAGACCTCTGGTCTGTGGG CTGCATCCTGTATGAGCTGGCTGTCGGCACGCCTCCCTTCTACACCACCAGCATCTTTCAGCTGGTTAGC CTCATTCTCAAGGACCCTGTGCGCTGGCCCTCCACCATTAGTTCCTGCTTCAAGAACTTCTTGCAGGGGC TGCTCACCAAGGACCCCCGGCAGCGTCTGTCCTGGCCAGACCTCTTACATCACCCCTTTATTGCCGGCCG TGTCACCATCATAACTGAACCAGCAGGCTCCGATTTGGGCACCCCATTTACTAGTCGCCTACCCCCAGAA CTTCAGGTCCTCAAGGATGAACAGGCGCATCGGCTCGCACCCAAGGGTAACCAGTCTCGCATCCTGCGCC AGGCCTGTAAACTCATGGCTGAAGAAGCCAAGCAGAAGGAAGACCAAAATGCAGGATCTGCCCTTGAACA AGAAGACGGGCTCTGCAAGGTGACACCCAGCACAGCCCCCGTGCCTGGACTAAAGGCCACTCCTCAGGAG TCAAGCCTCTTGGCTGGTATACTGGCTTCAGAAATGAAGAACAACTGGGAAGACTGGGGGGCTGGAGAAG CACCCCGTACCTCTCGGGAAAACCACATCAACCTGGAGTGTGAACAAGGCTTCCCAGAGCCGAGGCCAGA GGCGATGGGCCGGCAGAGCACTGATGTAGTGGATCCTGAAAATGAGGAGCCAGACAGTGATGATGAGTGG CAACGCCTACTAGAGACCAGCGAGCCTGGGCCTGTGCAGCTGAAGTCCCCCCTCACCCTGTTGTGTAACC CTGACTTCTGCCAGCGCATCCAGAGTCAGCTGCGCGGGACTGGCGAGCAGATCCTGAAAGGCGTTCTGGA TGGTGTGTCCCACCTCCTTCCTGTACTCCGCATCCTGAGTAGTCTCCTATCCAGCTGCAATGACTCTGTG CTCTTGTATTCCTTCTGCCAGGAGGCAGGACTGCCTGAGCTGCCTCTCAGCCTTCTTAGGTACAGCCAGG AGAGTAGCAGCATCCAGCAGCAACCTTGGTATGGGGCGCTCTTACGGGACCTGGTGGCTGTGGTTCAGGC CTACTTTTCGTGCACCTTCAATCTGGAGAGGAGCCAGACAGGTGACAGCCTACAGGTGTTTCAGGAGGCC GCCAGCCTCTTTTTGGACCTGTTGGGGAAGCTGCTGGCCCAATCAGATGATTCCGAGCAGACATTTCGAA GGGATAGCCTTATGTGCTTTGCTGTCTTGTGTGAAGCTGTGGACGGAAACAGCTGGGCCGTCTCCAAAGC CTTCTACTCCAGCCTGTTGACCACACAGCGCGCTGTGTTGGATGGCCTCCTTCACGGCCTGACAGTCCCA CAGCTTCCTTTCCACACACCACCAGGAGCCCCACAAGTGAGCCAGCCGCTGCGGGAGCAGAGTGAAGATG TACCTGGAGCCATCTCTTCTGCCCTGGCAGCCATGTGTACTGCTCCTGTGGGGCTGCCCAGCTGTTGGGA TGCCAAGGAGCAGGTCTCTTGGCATTTGGCCAACCAGCTAACTGAAGACAGCAGCCAACTGAGGCCATCC CTCATCTCTGGTCTGCGACACCATGTCCTGTGCCTCCACCTTCTCAAGGTTCTCTACGCCTGCTGCTATA TTAGTGAGCGCCTGTGCCATATTCTGGGGCAAGAGCCCCTGGCCTTGGAGTCGCTGCTGATGTTAGTCCA GGGCAAGGTGAAAGTAGCAGATTGGGAAGAGTCCACTGAGGTGGCACTTTACCTCCTGTCCCTTCTTGTC TTTCGGCTCCAAGATCTGCCTTCAGGGATGGAGAAGCTGGGCAGTGAGGTTGCTACTCTCTTTACCCACT CACATGTCGTCTCTCTTGTGAATGCGGCAGCCTGTCTCTTAGGACAGCTTGGTCAGCAAGGGGTGACCTT TGACCTCCAGCCCCGGGAATGGATTGCTGCAGCTGCACATGCCTTGTCTGCCCCTGCAGAGGTCCGGCTG ACTCCGCCGTATAGTTGTGGCTTCTATGATGGCCTCCTCATTCTCCTGCTTCAGCTTCTTATGCAGGGGA AGCCTGGCCTGATCAGGGATGTGGTTGGTTCAGAGGTGTGGACCATTCTGTGGCACCGCTTTTCCATGGC CCTGAGGCTGCCCGAGGAGGTGTCTGCCCAGGAAGACGACCTGCTACTATCAAGTCCCTCCAGCCTAGAG CCAGACTGGACACTGATTTCACCCCAAGGCATGGCAGCCTTGCTGAGCCTGGCCATGGCCATCTTCACCC AGGAGTCCCAGTTATGCCTGAGCCACCTGTCCCAGCATGGCAGTGTCCTCATGCTGACCCTGAAGCACCT GCTTTCACCCAGCTTCTTGCACCACCTGAGCCAGGCGCCGCAGGGGCCCGAGTTTCTCCCCGTTGTGGTG CTCTCCGTGTGCAAGCTGCTCTGCTTCCCTTTTGCCCTGGATGTGGATGCTGACCTCCTTGTAGGTGTCT TGGCTGACCTCAGGGCCTCGGAAGTGGTAGTCTGCCTGCTGCAGGTCTGCTGCCACCACCTTTCGTTGTT ACAAGCAGAGCTGCCCATTGGCCTCCTTACACGCCTGGCCCTCACGGATTCTGCCTCTCTCAGCGGCCGC TCGCTACCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR211549 representing BC043103 Red=Cloning site Green=Tags(s) MEKYHVLEMIGEGSFGRVYKGRKKYSAQVVALKFIPKLGRSEKELRNLQREIEIMRGLWHPNIVHMLDSF ETDKEVVVVTDYAEGELFQILEDDGKLPEDQVQAIAAQLVSALYYLHSHRILHRDMKPQNILLAKGGGIK LCDFGFARAMSTNTMVLTSIKGTPLYMSPELVEERPYDHTADLWSVGCILYELAVGTPPFYTTSIFQLVS LILKDPVRWPSTISSCFKNFLQGLLTKDPRQRLSWPDLLHHPFIAGRVTIITEPAGSDLGTPFTSRLPPE LQVLKDEQAHRLAPKGNQSRILRQACKLMAEEAKQKEDQNAGSALEQEDGLCKVTPSTAPVPGLKATPQE SSLLAGILASEMKNNWEDWGAGEAPRTSRENHINLECEQGFPEPRPEAMGRQSTDVVDPENEEPDSDDEW QRLLETSEPGPVQLKSPLTLLCNPDFCQRIQSQLRGTGEQILKGVLDGVSHLLPVLRILSSLLSSCNDSV LLYSFCQEAGLPELPLSLLRYSQESSSIQQQPWYGALLRDLVAVVQAYFSCTFNLERSQTGDSLQVFQEA ASLFLDLLGKLLAQSDDSEQTFRRDSLMCFAVLCEAVDGNSWAVSKAFYSSLLTTQRAVLDGLLHGLTVP QLPFHTPPGAPQVSQPLREQSEDVPGAISSALAAMCTAPVGLPSCWDAKEQVSWHLANQLTEDSSQLRPS LISGLRHHVLCLHLLKVLYACCYISERLCHILGQEPLALESLLMLVQGKVKVADWEESTEVALYLLSLLV FRLQDLPSGMEKLGSEVATLFTHSHVVSLVNAAACLLGQLGQQGVTFDLQPREWIAAAAHALSAPAEVRL TPPYSCGFYDGLLILLLQLLMQGKPGLIRDVVGSEVWTILWHRFSMALRLPEEVSAQEDDLLLSSPSSLE PDWTLISPQGMAALLSLAMAIFTQESQLCLSHLSQHGSVLMLTLKHLLSPSFLHHLSQAPQGPEFLPVVV LSVCKLLCFPFALDVDADLLVGVLADLRASEVVVCLLQVCCHHLSLLQAELPIGLLTRLALTDSASLSGR SLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	BC043103
ORF Size	3159 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC043103.1
RefSeq Size	3378 bp
RefSeq ORF	3161 bp
Locus ID	269209
MW	116.2 kDa
Gene Summary	Serine/threonine protein kinase which plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors. Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur. Required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Essential for construction of the central pair apparatus of motile cilia.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC223136	Stk36 (untagged) - Mouse serine/threonine kinase 36 (fused homolog, Drosophila) (cDNA clone MGC:58023 IMAGE:6406485), complete, (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 12,920.00
MG211549	Stk36 (tGFP-tagged) - Mouse serine/threonine kinase 36 (fused homolog, Drosophila) (cDNA clone MGC:58023 IMAGE:6406485), complete	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,560.00
MR211549L3	Lenti ORF clone of Stk36 (Myc-DDK-tagged) - Mouse serine/threonine kinase 36 (fused homolog, Drosophila) (cDNA clone MGC:58023 IMAGE:6406485), complete	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,890.00
MR211549L4	Lenti ORF clone of Stk36 (mGFP-tagged) - Mouse serine/threonine kinase 36 (fused homolog, Drosophila) (cDNA clone MGC:58023 IMAGE:6406485), complete	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,890.00