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Stk36 (BC043103) Mouse Tagged ORF Clone
CAT＃: MG211549
- TrueORF®
Stk36 (tGFP-tagged) - Mouse serine/threonine kinase 36 (fused homolog, Drosophila) (cDNA clone MGC:58023 IMAGE:6406485), complete
ORF Plasmid: DDK
"BC043103" in other vectors (4)
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CNY 6,560.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FU, MGC58023, B930045J24 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211549 representing BC043103
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAAGTACCACGTTTTGGAGATGATTGGAGAAGGCTCTTTTGGGAGAGTGTATAAGGGCCGAAAAA AATACAGTGCTCAGGTGGTGGCCTTGAAGTTCATCCCCAAACTGGGGCGCTCAGAGAAAGAGCTGAGGAA TCTGCAACGAGAGATTGAAATCATGCGGGGTCTGTGGCATCCCAACATTGTGCATATGCTCGACAGCTTT GAGACTGACAAAGAGGTGGTGGTGGTGACAGACTACGCTGAAGGAGAACTCTTTCAGATTCTGGAAGATG ATGGAAAACTTCCTGAAGACCAGGTTCAGGCCATCGCTGCCCAGTTGGTGTCAGCTCTGTACTACCTGCA TTCCCACCGCATCCTACACCGCGACATGAAACCGCAGAACATTCTTCTCGCCAAGGGTGGTGGCATTAAA CTTTGTGACTTTGGATTCGCCCGAGCTATGAGCACCAACACCATGGTGCTGACGTCGATCAAAGGCACAC CGCTCTATATGTCTCCAGAGCTGGTGGAGGAGCGACCATATGACCACACCGCAGACCTCTGGTCTGTGGG CTGCATCCTGTATGAGCTGGCTGTCGGCACGCCTCCCTTCTACACCACCAGCATCTTTCAGCTGGTTAGC CTCATTCTCAAGGACCCTGTGCGCTGGCCCTCCACCATTAGTTCCTGCTTCAAGAACTTCTTGCAGGGGC TGCTCACCAAGGACCCCCGGCAGCGTCTGTCCTGGCCAGACCTCTTACATCACCCCTTTATTGCCGGCCG TGTCACCATCATAACTGAACCAGCAGGCTCCGATTTGGGCACCCCATTTACTAGTCGCCTACCCCCAGAA CTTCAGGTCCTCAAGGATGAACAGGCGCATCGGCTCGCACCCAAGGGTAACCAGTCTCGCATCCTGCGCC AGGCCTGTAAACTCATGGCTGAAGAAGCCAAGCAGAAGGAAGACCAAAATGCAGGATCTGCCCTTGAACA AGAAGACGGGCTCTGCAAGGTGACACCCAGCACAGCCCCCGTGCCTGGACTAAAGGCCACTCCTCAGGAG TCAAGCCTCTTGGCTGGTATACTGGCTTCAGAAATGAAGAACAACTGGGAAGACTGGGGGGCTGGAGAAG CACCCCGTACCTCTCGGGAAAACCACATCAACCTGGAGTGTGAACAAGGCTTCCCAGAGCCGAGGCCAGA GGCGATGGGCCGGCAGAGCACTGATGTAGTGGATCCTGAAAATGAGGAGCCAGACAGTGATGATGAGTGG CAACGCCTACTAGAGACCAGCGAGCCTGGGCCTGTGCAGCTGAAGTCCCCCCTCACCCTGTTGTGTAACC CTGACTTCTGCCAGCGCATCCAGAGTCAGCTGCGCGGGACTGGCGAGCAGATCCTGAAAGGCGTTCTGGA TGGTGTGTCCCACCTCCTTCCTGTACTCCGCATCCTGAGTAGTCTCCTATCCAGCTGCAATGACTCTGTG CTCTTGTATTCCTTCTGCCAGGAGGCAGGACTGCCTGAGCTGCCTCTCAGCCTTCTTAGGTACAGCCAGG AGAGTAGCAGCATCCAGCAGCAACCTTGGTATGGGGCGCTCTTACGGGACCTGGTGGCTGTGGTTCAGGC CTACTTTTCGTGCACCTTCAATCTGGAGAGGAGCCAGACAGGTGACAGCCTACAGGTGTTTCAGGAGGCC GCCAGCCTCTTTTTGGACCTGTTGGGGAAGCTGCTGGCCCAATCAGATGATTCCGAGCAGACATTTCGAA GGGATAGCCTTATGTGCTTTGCTGTCTTGTGTGAAGCTGTGGACGGAAACAGCTGGGCCGTCTCCAAAGC CTTCTACTCCAGCCTGTTGACCACACAGCGCGCTGTGTTGGATGGCCTCCTTCACGGCCTGACAGTCCCA CAGCTTCCTTTCCACACACCACCAGGAGCCCCACAAGTGAGCCAGCCGCTGCGGGAGCAGAGTGAAGATG TACCTGGAGCCATCTCTTCTGCCCTGGCAGCCATGTGTACTGCTCCTGTGGGGCTGCCCAGCTGTTGGGA TGCCAAGGAGCAGGTCTCTTGGCATTTGGCCAACCAGCTAACTGAAGACAGCAGCCAACTGAGGCCATCC CTCATCTCTGGTCTGCGACACCATGTCCTGTGCCTCCACCTTCTCAAGGTTCTCTACGCCTGCTGCTATA TTAGTGAGCGCCTGTGCCATATTCTGGGGCAAGAGCCCCTGGCCTTGGAGTCGCTGCTGATGTTAGTCCA GGGCAAGGTGAAAGTAGCAGATTGGGAAGAGTCCACTGAGGTGGCACTTTACCTCCTGTCCCTTCTTGTC TTTCGGCTCCAAGATCTGCCTTCAGGGATGGAGAAGCTGGGCAGTGAGGTTGCTACTCTCTTTACCCACT CACATGTCGTCTCTCTTGTGAATGCGGCAGCCTGTCTCTTAGGACAGCTTGGTCAGCAAGGGGTGACCTT TGACCTCCAGCCCCGGGAATGGATTGCTGCAGCTGCACATGCCTTGTCTGCCCCTGCAGAGGTCCGGCTG ACTCCGCCGTATAGTTGTGGCTTCTATGATGGCCTCCTCATTCTCCTGCTTCAGCTTCTTATGCAGGGGA AGCCTGGCCTGATCAGGGATGTGGTTGGTTCAGAGGTGTGGACCATTCTGTGGCACCGCTTTTCCATGGC CCTGAGGCTGCCCGAGGAGGTGTCTGCCCAGGAAGACGACCTGCTACTATCAAGTCCCTCCAGCCTAGAG CCAGACTGGACACTGATTTCACCCCAAGGCATGGCAGCCTTGCTGAGCCTGGCCATGGCCATCTTCACCC AGGAGTCCCAGTTATGCCTGAGCCACCTGTCCCAGCATGGCAGTGTCCTCATGCTGACCCTGAAGCACCT GCTTTCACCCAGCTTCTTGCACCACCTGAGCCAGGCGCCGCAGGGGCCCGAGTTTCTCCCCGTTGTGGTG CTCTCCGTGTGCAAGCTGCTCTGCTTCCCTTTTGCCCTGGATGTGGATGCTGACCTCCTTGTAGGTGTCT TGGCTGACCTCAGGGCCTCGGAAGTGGTAGTCTGCCTGCTGCAGGTCTGCTGCCACCACCTTTCGTTGTT ACAAGCAGAGCTGCCCATTGGCCTCCTTACACGCCTGGCCCTCACGGATTCTGCCTCTCTCAGCGGCCGC TCGCTACCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211549 representing BC043103
Red=Cloning site Green=Tags(s) MEKYHVLEMIGEGSFGRVYKGRKKYSAQVVALKFIPKLGRSEKELRNLQREIEIMRGLWHPNIVHMLDSF ETDKEVVVVTDYAEGELFQILEDDGKLPEDQVQAIAAQLVSALYYLHSHRILHRDMKPQNILLAKGGGIK LCDFGFARAMSTNTMVLTSIKGTPLYMSPELVEERPYDHTADLWSVGCILYELAVGTPPFYTTSIFQLVS LILKDPVRWPSTISSCFKNFLQGLLTKDPRQRLSWPDLLHHPFIAGRVTIITEPAGSDLGTPFTSRLPPE LQVLKDEQAHRLAPKGNQSRILRQACKLMAEEAKQKEDQNAGSALEQEDGLCKVTPSTAPVPGLKATPQE SSLLAGILASEMKNNWEDWGAGEAPRTSRENHINLECEQGFPEPRPEAMGRQSTDVVDPENEEPDSDDEW QRLLETSEPGPVQLKSPLTLLCNPDFCQRIQSQLRGTGEQILKGVLDGVSHLLPVLRILSSLLSSCNDSV LLYSFCQEAGLPELPLSLLRYSQESSSIQQQPWYGALLRDLVAVVQAYFSCTFNLERSQTGDSLQVFQEA ASLFLDLLGKLLAQSDDSEQTFRRDSLMCFAVLCEAVDGNSWAVSKAFYSSLLTTQRAVLDGLLHGLTVP QLPFHTPPGAPQVSQPLREQSEDVPGAISSALAAMCTAPVGLPSCWDAKEQVSWHLANQLTEDSSQLRPS LISGLRHHVLCLHLLKVLYACCYISERLCHILGQEPLALESLLMLVQGKVKVADWEESTEVALYLLSLLV FRLQDLPSGMEKLGSEVATLFTHSHVVSLVNAAACLLGQLGQQGVTFDLQPREWIAAAAHALSAPAEVRL TPPYSCGFYDGLLILLLQLLMQGKPGLIRDVVGSEVWTILWHRFSMALRLPEEVSAQEDDLLLSSPSSLE PDWTLISPQGMAALLSLAMAIFTQESQLCLSHLSQHGSVLMLTLKHLLSPSFLHHLSQAPQGPEFLPVVV LSVCKLLCFPFALDVDADLLVGVLADLRASEVVVCLLQVCCHHLSLLQAELPIGLLTRLALTDSASLSGR SLP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC043103 |
| ORF Size | 3159 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC043103.1 |
| RefSeq Size | 3378 bp |
| RefSeq ORF | 3161 bp |
| Locus ID | 269209 |
| Gene Summary | Serine/threonine protein kinase which plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors. Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur. Required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Essential for construction of the central pair apparatus of motile cilia.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223136 | Stk36 (untagged) - Mouse serine/threonine kinase 36 (fused homolog, Drosophila) (cDNA clone MGC:58023 IMAGE:6406485), complete, (10ug) |
CNY 12,920.00 |
|
| MR211549 | Stk36 (Myc-DDK-tagged) - Mouse serine/threonine kinase 36 (fused homolog, Drosophila) (cDNA clone MGC:58023 IMAGE:6406485), complete |
CNY 7,336.00 |
|
| MR211549L3 | Lenti ORF clone of Stk36 (Myc-DDK-tagged) - Mouse serine/threonine kinase 36 (fused homolog, Drosophila) (cDNA clone MGC:58023 IMAGE:6406485), complete |
CNY 7,890.00 |
|
| MR211549L4 | Lenti ORF clone of Stk36 (mGFP-tagged) - Mouse serine/threonine kinase 36 (fused homolog, Drosophila) (cDNA clone MGC:58023 IMAGE:6406485), complete |
CNY 7,890.00 |
