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Cit (BC023775) Mouse Tagged ORF Clone
CAT＃: MR211488
- TrueORF®
Cit (Myc-DDK-tagged) - Mouse citron (cDNA clone MGC:38411 IMAGE:5346007)
ORF Plasmid: tGFP
"BC023775" in other vectors (4)
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CNY 10,260.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CRIK, CRIK-SK, citron-N, Cit-k |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211488 representing BC023775
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTCTGAAGACGACATGCACCATGTTGGAGGAGCAGGTCCTGGACCTGGAGGCCCTGAACGACG AGCTGCTGGAGAAGGAGCGCCAGTGGGAGGCCTGGCGGAGCGTCCTTGGCGACGAGAAGTCCCAGTTCGA GTGTCGAGTTCGAGAGCTACAGAGGATGCTGGACACCGAGAAGCAGAGCAGGGCTAGGGCCGATCAGCGG ATCACCGAGTCGCGCCAGGTGGTGGAGTTGGCGGTGAAGGAACACAAGGCCGAGATTCTTGCTCTGCAGC AGGCTCTCAAGGAGCAGAAGCTCAAAGCCGAGAGCCTGTCGGACAAGCTCAACGACCTGGAGAAGAAGCA CGCCATGCTGGAGATGAACGCCCGGAGCTTACAGCAGAAACTAGAGACAGAGCGGGAGCTCAAACAGAGG CTTCTGGAGGAGCAAGCCAAATTGCAGCAGCAGATGGACCTGCAGAAGAACCACATCTTCAGACTGACGC AAGGGCTGCAGGAGGCGCTGGACCGGGCGGATCTGCTGAAGACAGAAAGGAGCGACCTGGAGTACCAGCT GGAAAACATTCAGGTTCTCTACTCTCACGAGAAAGTGAAAATGGAAGGCACAATCTCTCAGCAAACCAAA CTCATTGATTTCCTGCAAGCCAAAATGGACCAGCCTGCTAAAAAGAAAAAGGGTTTATTTAGTCGACGGA AAGAGGACCCTGCTTTGCCCACACAGGTTCCTCTGCAGTACAATGAGCTGAAGCTAGCCCTGGAAAAGGA GAAAGCCCGATGCGCGGAGCTGGAGGAGGCCCTTCAGAAGACCCGCATCGAACTCCGCTCTGCGCGGGAG GAAGCTGCCCACCGCAAAGCCACGGACCACCCGCACCCGTCTACGCCAGCCACTGCGAGGCAGCAGATCG CCATGTCTGCCATTGTGCGGTCGCCCGAGCACCAGCCCAGTGCCATGAGCCTGCTTGCCCCACCATCCAG CCGCAGAAAGGAGTCGTCAACTCCAGAGGAATTCAGCCGGCGTCTGAAAGAGCGCATGCACCACAACATC CCTCACCGGTTTAATGTGGGCCTGAACATGAGAGCCACCAAGTGCGCCGTGTGTCTGGATACTGTGCACT TTGGACGCCAGGCATCCAAATGCCTCGAATGTCAAGTGATGTGTCACCCCAAATGCTCCACCTGCTTGCC CGCTACCTGTGGCCTGCCAGCTGAATATGCCACACACTTCACTGAGGCCTTCTGCCGGGACAAAATGAAC TCCCCGGGGCTCCAGAGCAAGGAGCCTGGCAGCAGCTTGCACCTGGAAGGGTGGATGAAAGTGCCCAGAA ATAACAAACGGGGACAGCAAGGCTGGGACAGGAAGTACATTGTTCTGGAGGGGTCAAAAGTCCTCATCTA TGACAATGAAGCCAGAGAAGCTGGACAGAGGCCGGTGGAAGAATTTGAGCTGTGCCTTCCTGACGGGGAC GTATCTATTCATGGTGCCGTTGGTGCTTCAGAACTTGCAAATACCGCCAAAGCAGATGTCCCATACATCC TGAAGATGGAGTCTCACCCACACACCACCTGCTGGCCTGGGAGAACCCTCTACTTGCTAGCACCCAGCTT CCCCGACAAGCAGCGCTGGGTCACCGCCTTAGAATCTGTCGTCGCAGGTGGGAGAGTTTCTAGGGAAAAG GCCGAAGCCGATGCTGCACGTGACCATACTTCGAGTGAGCATCAGCCAGTGTGGGTTGAGAAATTACTTG GAAACTCTCTGCTGAAACTGGAGGGCGATGACCGGCTTGACATGAACTGCACCCTGCCCTTCAGTGACCA GGTGGTGCTGGTGGGCACGGAGGAAGGCCTCTACGCCCTGAATGTCTTGAAAAACTCCTTAACCCACATC CCAGGGATTGGCGCAGTCTTCCAAATTTACATCATCAAGGACCTGGAGAAGCTGCTCATGATAGCAGGGG AAGAGCGGGCTCTGTGCCTGGTGGACGTGAAGAAGGTGAAGCAGTCCCTGGCGCAGTCACACCTGCCTGC CCAGCCCGACGTCTCCCCCAACATATTCGAAGCCGTCAAAGGCTGCCACTTGTTCGCTGCTGGCAAGATC GAGAACAGCCTGTGCATCTGCGCCGCTATGCCAAGCAAAGTCGTCATCCTCCGCTACAATGACAACCTCA GCAAGTACTGCATCCGCAAGGAGATCGAGACCTCAGAGCCCTGCAGCTGTATCCACTTCACCAACTACAG CATCCTCATTGGGACCAACAAATTCTATGAGATCGACATGAAGCAGTACACGCTTGATGAGTTCCTGGAC AAGAACGACCATTCCTTGGCACCTGCTGTGTTCGCCTCCTCGTCCAACAGCTTCCCTGTCTCCATTGTGC AGGCGAACAGCGCCGGGCAGCGAGAAGAATACCTGCTGTGCTTCCACGAATTTGGGGTGTTCGTGGATTC TTACGGAAGACGTAGCCGCACAGATGATCTTAAGTGGAGTCGCTTACCTCTGGCCTTCGCCTACAGAGAA CCTTATCTGTTTGTGACTCACTTCAACTCCCTGGAAGTCATTGAGATCCAGGCCAGATCCTCACTGGGGA GCCCTGCCCGAGCATATCTGGAAATTCCAAACCCTCGCTACCTGGGCCCCGCGATTTCCTCCGGAGCGAT TTACCTGGCCTCCTCATACCAGGACAAGTTAAGGGTCATATGCTGCAAAGGAAACCTCGTGAAGGAGTCA GGCACTGAGCAGCACCGGGTGCCCTCCACCTCCCGCAGCCCCAACAAGCGAGGCCCACCAACATACAACG AGCACATCACCAAACGCGTGGCCTCCAGCCCGGCGCCACCGGAAGGCCCCAGCCACCCCCGAGAGCCAAG CACACCGCACCGCTACCGAGACAGAGAGGGTCGGACAGAGCTGCGCAGGGACAAGTCTCCAGGCCGCCCT CTGGAGCGGGAGAAGTCCCCAGGCCGAATGCTCAGCACTAGGAGAGAGCGGTCCCCAGGGAGACTGTTTG AAGACAGCAGCAGGGGCCGGCTGCCTGCAGGAGCAGTGAGGACCCCACTGTCCCAGGTTAACAAGGTCTG GGACCAGTCTTCAGTA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR211488 representing BC023775
Red=Cloning site Green=Tags(s) MEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARADQR ITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQR LLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQTK LIDFLQAKMDQPAKKKKGLFSRRKEDPALPTQVPLQYNELKLALEKEKARCAELEEALQKTRIELRSARE EAAHRKATDHPHPSTPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNI PHRFNVGLNMRATKCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMN SPGLQSKEPGSSLHLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGD VSIHGAVGASELANTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREK AEADAARDHTSSEHQPVWVEKLLGNSLLKLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHI PGIGAVFQIYIIKDLEKLLMIAGEERALCLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKI ENSLCICAAMPSKVVILRYNDNLSKYCIRKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLD KNDHSLAPAVFASSSNSFPVSIVQANSAGQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYRE PYLFVTHFNSLEVIEIQARSSLGSPARAYLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKES GTEQHRVPSTSRSPNKRGPPTYNEHITKRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRP LEREKSPGRMLSTRRERSPGRLFEDSSRGRLPAGAVRTPLSQVNKVWDQSSV SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC023775 |
| ORF Size | 3096 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC023775.1 |
| RefSeq Size | 3506 bp |
| RefSeq ORF | 3098 bp |
| Locus ID | 12704 |
| MW | 128.5 kDa |
| Gene Summary | Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody. Probable RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. Displays serine/threonine protein kinase activity. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223050 | Cit (untagged) - Mouse citron (cDNA clone MGC:38411 IMAGE:5346007), (10ug) |
CNY 12,920.00 |
|
| MG211488 | Cit (tGFP-tagged) - Mouse citron (cDNA clone MGC:38411 IMAGE:5346007) |
CNY 11,310.00 |
|
| MR211488L3 | Lenti ORF clone of Cit (Myc-DDK-tagged) - Mouse citron (cDNA clone MGC:38411 IMAGE:5346007) |
CNY 12,160.00 |
|
| MR211488L4 | Lenti ORF clone of Cit (mGFP-tagged) - Mouse citron (cDNA clone MGC:38411 IMAGE:5346007) |
CNY 12,160.00 |
