Cit (BC023775) Mouse Untagged Clone

CAT#: MC223050

Cit (untagged) - Mouse citron (cDNA clone MGC:38411 IMAGE:5346007), (10ug)



  "BC023775" in other vectors (4)

CNY 12,920.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms CRIK, CRIK-SK, citron-N, Cit-k
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC023775
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCTCTGAAGACGACATGCACCATGTTGGAGGAGCAGGTCCTGGACCTGGAGGCCCTGAACGACG
AGCTGCTGGAGAAGGAGCGCCAGTGGGAGGCCTGGCGGAGCGTCCTTGGCGACGAGAAGTCCCAGTTCGA
GTGTCGAGTTCGAGAGCTACAGAGGATGCTGGACACCGAGAAGCAGAGCAGGGCTAGGGCCGATCAGCGG
ATCACCGAGTCGCGCCAGGTGGTGGAGTTGGCGGTGAAGGAACACAAGGCCGAGATTCTTGCTCTGCAGC
AGGCTCTCAAGGAGCAGAAGCTCAAAGCCGAGAGCCTGTCGGACAAGCTCAACGACCTGGAGAAGAAGCA
CGCCATGCTGGAGATGAACGCCCGGAGCTTACAGCAGAAACTAGAGACAGAGCGGGAGCTCAAACAGAGG
CTTCTGGAGGAGCAAGCCAAATTGCAGCAGCAGATGGACCTGCAGAAGAACCACATCTTCAGACTGACGC
AAGGGCTGCAGGAGGCGCTGGACCGGGCGGATCTGCTGAAGACAGAAAGGAGCGACCTGGAGTACCAGCT
GGAAAACATTCAGGTTCTCTACTCTCACGAGAAAGTGAAAATGGAAGGCACAATCTCTCAGCAAACCAAA
CTCATTGATTTCCTGCAAGCCAAAATGGACCAGCCTGCTAAAAAGAAAAAGGGTTTATTTAGTCGACGGA
AAGAGGACCCTGCTTTGCCCACACAGGTTCCTCTGCAGTACAATGAGCTGAAGCTAGCCCTGGAAAAGGA
GAAAGCCCGATGCGCGGAGCTGGAGGAGGCCCTTCAGAAGACCCGCATCGAACTCCGCTCTGCGCGGGAG
GAAGCTGCCCACCGCAAAGCCACGGACCACCCGCACCCGTCTACGCCAGCCACTGCGAGGCAGCAGATCG
CCATGTCTGCCATTGTGCGGTCGCCCGAGCACCAGCCCAGTGCCATGAGCCTGCTTGCCCCACCATCCAG
CCGCAGAAAGGAGTCGTCAACTCCAGAGGAATTCAGCCGGCGTCTGAAAGAGCGCATGCACCACAACATC
CCTCACCGGTTTAATGTGGGCCTGAACATGAGAGCCACCAAGTGCGCCGTGTGTCTGGATACTGTGCACT
TTGGACGCCAGGCATCCAAATGCCTCGAATGTCAAGTGATGTGTCACCCCAAATGCTCCACCTGCTTGCC
CGCTACCTGTGGCCTGCCAGCTGAATATGCCACACACTTCACTGAGGCCTTCTGCCGGGACAAAATGAAC
TCCCCGGGGCTCCAGAGCAAGGAGCCTGGCAGCAGCTTGCACCTGGAAGGGTGGATGAAAGTGCCCAGAA
ATAACAAACGGGGACAGCAAGGCTGGGACAGGAAGTACATTGTTCTGGAGGGGTCAAAAGTCCTCATCTA
TGACAATGAAGCCAGAGAAGCTGGACAGAGGCCGGTGGAAGAATTTGAGCTGTGCCTTCCTGACGGGGAC
GTATCTATTCATGGTGCCGTTGGTGCTTCAGAACTTGCAAATACCGCCAAAGCAGATGTCCCATACATCC
TGAAGATGGAGTCTCACCCACACACCACCTGCTGGCCTGGGAGAACCCTCTACTTGCTAGCACCCAGCTT
CCCCGACAAGCAGCGCTGGGTCACCGCCTTAGAATCTGTCGTCGCAGGTGGGAGAGTTTCTAGGGAAAAG
GCCGAAGCCGATGCTGCACGTGACCATACTTCGAGTGAGCATCAGCCAGTGTGGGTTGAGAAATTACTTG
GAAACTCTCTGCTGAAACTGGAGGGCGATGACCGGCTTGACATGAACTGCACCCTGCCCTTCAGTGACCA
GGTGGTGCTGGTGGGCACGGAGGAAGGCCTCTACGCCCTGAATGTCTTGAAAAACTCCTTAACCCACATC
CCAGGGATTGGCGCAGTCTTCCAAATTTACATCATCAAGGACCTGGAGAAGCTGCTCATGATAGCAGGGG
AAGAGCGGGCTCTGTGCCTGGTGGACGTGAAGAAGGTGAAGCAGTCCCTGGCGCAGTCACACCTGCCTGC
CCAGCCCGACGTCTCCCCCAACATATTCGAAGCCGTCAAAGGCTGCCACTTGTTCGCTGCTGGCAAGATC
GAGAACAGCCTGTGCATCTGCGCCGCTATGCCAAGCAAAGTCGTCATCCTCCGCTACAATGACAACCTCA
GCAAGTACTGCATCCGCAAGGAGATCGAGACCTCAGAGCCCTGCAGCTGTATCCACTTCACCAACTACAG
CATCCTCATTGGGACCAACAAATTCTATGAGATCGACATGAAGCAGTACACGCTTGATGAGTTCCTGGAC
AAGAACGACCATTCCTTGGCACCTGCTGTGTTCGCCTCCTCGTCCAACAGCTTCCCTGTCTCCATTGTGC
AGGCGAACAGCGCCGGGCAGCGAGAAGAATACCTGCTGTGCTTCCACGAATTTGGGGTGTTCGTGGATTC
TTACGGAAGACGTAGCCGCACAGATGATCTTAAGTGGAGTCGCTTACCTCTGGCCTTCGCCTACAGAGAA
CCTTATCTGTTTGTGACTCACTTCAACTCCCTGGAAGTCATTGAGATCCAGGCCAGATCCTCACTGGGGA
GCCCTGCCCGAGCATATCTGGAAATTCCAAACCCTCGCTACCTGGGCCCCGCGATTTCCTCCGGAGCGAT
TTACCTGGCCTCCTCATACCAGGACAAGTTAAGGGTCATATGCTGCAAAGGAAACCTCGTGAAGGAGTCA
GGCACTGAGCAGCACCGGGTGCCCTCCACCTCCCGCAGCCCCAACAAGCGAGGCCCACCAACATACAACG
AGCACATCACCAAACGCGTGGCCTCCAGCCCGGCGCCACCGGAAGGCCCCAGCCACCCCCGAGAGCCAAG
CACACCGCACCGCTACCGAGACAGAGAGGGTCGGACAGAGCTGCGCAGGGACAAGTCTCCAGGCCGCCCT
CTGGAGCGGGAGAAGTCCCCAGGCCGAATGCTCAGCACTAGGAGAGAGCGGTCCCCAGGGAGACTGTTTG
AAGACAGCAGCAGGGGCCGGCTGCCTGCAGGAGCAGTGAGGACCCCACTGTCCCAGGTTAACAAGGTCTG
GGACCAGTCTTCAGTATAA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATA
TCCTGGATTACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-RsrII     
ACCN BC023775
Insert Size 3099 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC023775, AAH23775
RefSeq Size 3506 bp
RefSeq ORF 3098 bp
Locus ID 12704
Gene Summary Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody. Probable RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. Displays serine/threonine protein kinase activity. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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