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Mcm3 (BC031700) Mouse Tagged ORF Clone
CAT＃: MR210780
- TrueORF®
Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S
ORF Plasmid: tGFP
"BC031700" in other vectors (3)
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CNY 5,944.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | P1, p1.m |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210780 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCACAGTAGTGCTGGATGATGTGGAGCTGCGGGAAGCGCAGAGAGACTACTTGGACTTCCTGG ATGACGAGGAGGACCAAGGCATTTACCAGAACAAGGTTCGGGAACTGATCAGTGACAACCAGTATCGGCT GATTGTCAGCGTGAATGACCTGCGCAGGAAGAATGAAAAGAGGGCTAACCGCCTCCTGAACAATGCGTTT GAGGAGCTGGTTGCCTTCCAGCGGGCCTTGAAGGATTTCGTGGCCTCCATTGACGCCACCTACGCCAAGC AGTATGAGGAGTTCTACATAGGATTGGAAGGCAGCTTTGGCTCAAAGCACGTTTCTCCCCGGACTCTCAC TTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAAGGCATTGTTACTAAATGCTCTCTGGTTCGCCCCAAA GTCGTTCGCAGTGTTCACTACTGTCCTGCTACTAAGAAGACCATAGAGCGACGCTACTCTGACCTCACCA CCCTAGTGGCTTTCCCATCCAGTTCTGTTTATCCCACAAAGGATGAGGAGAACAACCCCCTGGAGACAGA GTATGGCCTGTCTGTCTACAAGGACCACCAGACCATCACCATCCAGGAGATGCCAGAGAAGGCCCCTGCT GGTCAGCTCCCTCGCTCCGTGGACGTCATTCTAGACGATGACCTGGTGGATAAAGTGAAGCCTGGTGACA GAATACAGGTGGTGGGGACCTACCGCTGCCTTCCAGGGAAGAAGGGCTGCTACACCTCGGGGACCTTCAG GACCGTCCTGATTGCCTGTAATGTGAAGCAGATGAGTAAGGATATTCAGCCTGCATTCTCTGCGGACGAT ATAGCCAAGATCAAGAAGTTCAGCAAAACTCGTTCCAAGGATGTCTTTGAGCAGCTGGCCCGGTCGTTAG CCCCGAGCATCCATGGGCATGACTATGTCAAGAAGGCAATCCTCTGCTTGCTCCTGGGAGGGGTGGAGCG GGAACTTGAAAACGGCAGCCACATCCGTGGAGACATCAATATTCTTCTAATAGGTGACCCCTCCGTTGCC AAGTCCCAGCTTCTGCGGTACGTGCTTTGCACGGCGCCCAGGGCCATTCCCACCACGGGACGGGGCTCCT CTGGAGTGGGTCTCACAGCTGCTGTCACTACAGACCAGGAAACAGGGGAGCGCCGCCTGGAAGCTGGTGC TATGGTCCTGGCTGACCGTGGAGTGGTTTGCATTGATGAGTTTGACAAGATGTCTGACATGGACCGCACA GCCATCCACGAGGTGATGGAGCAGGGTCGAGTGACCATTGCAAAGGCTGGCATCCATGCCCGACTCAATG CCCGCTGCAGTGTTTTGGCAGCTGCCAATCCAGTCTATGGCAGGTATGATCAGTATAAGACCCCCATGGA GAACATTGGGCTGCAGGACTCACTGCTGTCTAGATTTGACCTGCTCTTCATCATGCTGGATCAGATGGAT CCTGAACAGGATCGGGAGATCTCAGACCACGTCCTTAGGATGCACCAATACAGGGCCCCGGGGGAGCAGG ACGGTGATGCTTTGCCATTGGGTAGTTCAGTGGATATCCTGGCCACAGACGATCCTGACTTTACCCAGGA TGACCAGCAAGATACCCGCATTTATGAGAAGCATGACAGCCTTCTACATGGGACCAAGAAGAAAAAGGAG AAGATGGTGAGCGCAGCTTTTATGAAGAAGTACATTCACGTGGCCAAAATTATCAAGCCCACCCTGACAC AGGAGTCAGCAGCCTACATTGCAGAAGAATACTCACGCCTGCGCAGCCAGGACAGTATGAGCTCAGACAC TGCCCGGACATCTCCAGTTACAGCACGGACACTGGAAACTCTGATTCGACTGGCCACGGCCCATGCGAAG GCCCGGATGAGCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCCGTGGAGCTGGTTCAGTATGCTTATT TCAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGGCAAGTGAGGATGAATCAGACCTAGAAGATGA AGAGGAAAAGAGCCAGGAAGACACTGAGCAGAAGCGGAAAAGGAGGAAGACTCATGCCAAGGATGGAGAG TCCTATGACCCTTACGACTTCAGTGAAGCAGAGACGCAGATGCCTCAAGTGCACACCCCAAAGACTGACG ATTCCCAAGAGAAGACCGATGATTCTCAGGAGACCCAGGACTCCCAGAAAGTGGAACTCAGTGAGCCCAG GCTGAAGGCTTTTAAGGCGGCCCTCTTAGAAGTGTTCCAGGAAGCTCATGAGCAGTCTGTGGGCATGCTC CACCTCACAGAATCCATCAACCGGAATAGAGAAGAGCCTTTCTCCTCAGAGGAAATCCAGGCTTGCCTGA GCCGGATGCAGGATGATAACCAGGTCATGGTGTCTGAGGGCATCGTCTTCCTTATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210780 protein sequence
Red=Cloning site Green=Tags(s) MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQNKVRELISDNQYRLIVSVNDLRRKNEKRANRLLNNAF EELVAFQRALKDFVASIDATYAKQYEEFYIGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPK VVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPA GQLPRSVDVILDDDLVDKVKPGDRIQVVGTYRCLPGKKGCYTSGTFRTVLIACNVKQMSKDIQPAFSADD IAKIKKFSKTRSKDVFEQLARSLAPSIHGHDYVKKAILCLLLGGVERELENGSHIRGDINILLIGDPSVA KSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRT AIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMD PEQDREISDHVLRMHQYRAPGEQDGDALPLGSSVDILATDDPDFTQDDQQDTRIYEKHDSLLHGTKKKKE KMVSAAFMKKYIHVAKIIKPTLTQESAAYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAK ARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKASEDESDLEDEEEKSQEDTEQKRKRRKTHAKDGE SYDPYDFSEAETQMPQVHTPKTDDSQEKTDDSQETQDSQKVELSEPRLKAFKAALLEVFQEAHEQSVGML HLTESINRNREEPFSSEEIQACLSRMQDDNQVMVSEGIVFLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC031700 |
| ORF Size | 2436 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031700, AAH31700 |
| RefSeq Size | 2899 bp |
| RefSeq ORF | 2438 bp |
| Locus ID | 17215 |
| MW | 91.5 kDa |
| Gene Summary | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210780 | Mcm3 (tGFP-tagged) - Mouse minichromosome maintenance deficient 3 (S |
CNY 5,130.00 |
|
| MR210780L3 | Lenti ORF clone of Mcm3 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 3 (S |
CNY 6,560.00 |
|
| MR210780L4 | Lenti ORF clone of Mcm3 (mGFP-tagged) - Mouse minichromosome maintenance deficient 3 (S |
CNY 6,560.00 |
