Mcm3 (BC031700) Mouse Tagged ORF Clone

CAT#: MG210780

  • TrueORF®

Mcm3 (tGFP-tagged) - Mouse minichromosome maintenance deficient 3 (S

ORF Plasmid: DDK tGFP



  "BC031700" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,130.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms P1, p1.m
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG210780 representing BC031700
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCACAGTAGTGCTGGATGATGTGGAGCTGCGGGAAGCGCAGAGAGACTACTTGGACTTCCTGG
ATGACGAGGAGGACCAAGGCATTTACCAGAACAAGGTTCGGGAACTGATCAGTGACAACCAGTATCGGCT
GATTGTCAGCGTGAATGACCTGCGCAGGAAGAATGAAAAGAGGGCTAACCGCCTCCTGAACAATGCGTTT
GAGGAGCTGGTTGCCTTCCAGCGGGCCTTGAAGGATTTCGTGGCCTCCATTGACGCCACCTACGCCAAGC
AGTATGAGGAGTTCTACATAGGATTGGAAGGCAGCTTTGGCTCAAAGCACGTTTCTCCCCGGACTCTCAC
TTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAAGGCATTGTTACTAAATGCTCTCTGGTTCGCCCCAAA
GTCGTTCGCAGTGTTCACTACTGTCCTGCTACTAAGAAGACCATAGAGCGACGCTACTCTGACCTCACCA
CCCTAGTGGCTTTCCCATCCAGTTCTGTTTATCCCACAAAGGATGAGGAGAACAACCCCCTGGAGACAGA
GTATGGCCTGTCTGTCTACAAGGACCACCAGACCATCACCATCCAGGAGATGCCAGAGAAGGCCCCTGCT
GGTCAGCTCCCTCGCTCCGTGGACGTCATTCTAGACGATGACCTGGTGGATAAAGTGAAGCCTGGTGACA
GAATACAGGTGGTGGGGACCTACCGCTGCCTTCCAGGGAAGAAGGGCTGCTACACCTCGGGGACCTTCAG
GACCGTCCTGATTGCCTGTAATGTGAAGCAGATGAGTAAGGATATTCAGCCTGCATTCTCTGCGGACGAT
ATAGCCAAGATCAAGAAGTTCAGCAAAACTCGTTCCAAGGATGTCTTTGAGCAGCTGGCCCGGTCGTTAG
CCCCGAGCATCCATGGGCATGACTATGTCAAGAAGGCAATCCTCTGCTTGCTCCTGGGAGGGGTGGAGCG
GGAACTTGAAAACGGCAGCCACATCCGTGGAGACATCAATATTCTTCTAATAGGTGACCCCTCCGTTGCC
AAGTCCCAGCTTCTGCGGTACGTGCTTTGCACGGCGCCCAGGGCCATTCCCACCACGGGACGGGGCTCCT
CTGGAGTGGGTCTCACAGCTGCTGTCACTACAGACCAGGAAACAGGGGAGCGCCGCCTGGAAGCTGGTGC
TATGGTCCTGGCTGACCGTGGAGTGGTTTGCATTGATGAGTTTGACAAGATGTCTGACATGGACCGCACA
GCCATCCACGAGGTGATGGAGCAGGGTCGAGTGACCATTGCAAAGGCTGGCATCCATGCCCGACTCAATG
CCCGCTGCAGTGTTTTGGCAGCTGCCAATCCAGTCTATGGCAGGTATGATCAGTATAAGACCCCCATGGA
GAACATTGGGCTGCAGGACTCACTGCTGTCTAGATTTGACCTGCTCTTCATCATGCTGGATCAGATGGAT
CCTGAACAGGATCGGGAGATCTCAGACCACGTCCTTAGGATGCACCAATACAGGGCCCCGGGGGAGCAGG
ACGGTGATGCTTTGCCATTGGGTAGTTCAGTGGATATCCTGGCCACAGACGATCCTGACTTTACCCAGGA
TGACCAGCAAGATACCCGCATTTATGAGAAGCATGACAGCCTTCTACATGGGACCAAGAAGAAAAAGGAG
AAGATGGTGAGCGCAGCTTTTATGAAGAAGTACATTCACGTGGCCAAAATTATCAAGCCCACCCTGACAC
AGGAGTCAGCAGCCTACATTGCAGAAGAATACTCACGCCTGCGCAGCCAGGACAGTATGAGCTCAGACAC
TGCCCGGACATCTCCAGTTACAGCACGGACACTGGAAACTCTGATTCGACTGGCCACGGCCCATGCGAAG
GCCCGGATGAGCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCCGTGGAGCTGGTTCAGTATGCTTATT
TCAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGGCAAGTGAGGATGAATCAGACCTAGAAGATGA
AGAGGAAAAGAGCCAGGAAGACACTGAGCAGAAGCGGAAAAGGAGGAAGACTCATGCCAAGGATGGAGAG
TCCTATGACCCTTACGACTTCAGTGAAGCAGAGACGCAGATGCCTCAAGTGCACACCCCAAAGACTGACG
ATTCCCAAGAGAAGACCGATGATTCTCAGGAGACCCAGGACTCCCAGAAAGTGGAACTCAGTGAGCCCAG
GCTGAAGGCTTTTAAGGCGGCCCTCTTAGAAGTGTTCCAGGAAGCTCATGAGCAGTCTGTGGGCATGCTC
CACCTCACAGAATCCATCAACCGGAATAGAGAAGAGCCTTTCTCCTCAGAGGAAATCCAGGCTTGCCTGA
GCCGGATGCAGGATGATAACCAGGTCATGGTGTCTGAGGGCATCGTCTTCCTTATC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG210780 representing BC031700
Red=Cloning site Green=Tags(s)

MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQNKVRELISDNQYRLIVSVNDLRRKNEKRANRLLNNAF
EELVAFQRALKDFVASIDATYAKQYEEFYIGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPK
VVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPA
GQLPRSVDVILDDDLVDKVKPGDRIQVVGTYRCLPGKKGCYTSGTFRTVLIACNVKQMSKDIQPAFSADD
IAKIKKFSKTRSKDVFEQLARSLAPSIHGHDYVKKAILCLLLGGVERELENGSHIRGDINILLIGDPSVA
KSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRT
AIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMD
PEQDREISDHVLRMHQYRAPGEQDGDALPLGSSVDILATDDPDFTQDDQQDTRIYEKHDSLLHGTKKKKE
KMVSAAFMKKYIHVAKIIKPTLTQESAAYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAK
ARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKASEDESDLEDEEEKSQEDTEQKRKRRKTHAKDGE
SYDPYDFSEAETQMPQVHTPKTDDSQEKTDDSQETQDSQKVELSEPRLKAFKAALLEVFQEAHEQSVGML
HLTESINRNREEPFSSEEIQACLSRMQDDNQVMVSEGIVFLI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC031700
ORF Size 2438 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC031700, AAH31700
RefSeq Size 2899 bp
RefSeq ORF 2438 bp
Locus ID 17215
Gene Summary Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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