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Fgfr4 (NM_008011) Mouse Tagged ORF Clone
CAT＃: MR210769
- TrueORF®
Fgfr4 (Myc-DDK-tagged) - Mouse fibroblast growth factor receptor 4 (Fgfr4)
ORF Plasmid: tGFP
"NM_008011" in other vectors (6)
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CNY 8,776.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Fgfr-4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210769 representing NM_008011
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTGCTCTTGGCCCTGTTGAGCATCTTTCAGGGGACACCAGCTTTGTCCCTTGAGGCCTCTGAGG AAATGGAGCAGGAGCCCTGCCTAGCCCCAATCCTGGAGCAGCAAGAGCAGGTGTTGACGGTGGCCCTGGG GCAGCCTGTGAGGCTGTGCTGTGGGCGCACCGAGCGTGGTCGTCACTGGTACAAAGAGGGCAGCCGCCTA GCATCTGCTGGGCGAGTACGGGGTTGGAGAGGCCGCCTGGAGATCGCCAGCTTCCTTCCTGAGGATGCTG GCCGATACCTCTGCCTGGCCCGTGGCTCCATGACCGTCGTACACAATCTTACGTTGCTTATGGATGACTC CTTAACCTCCATCAGTAATGATGAAGACCCCAAGACACTCAGCAGCTCCTCGAGTGGTCATGTCTACCCA CAGCAAGCACCCTACTGGACACACCCCCAACGCATGGAGAAGAAACTGCATGCAGTGCCTGCCGGGAATA CTGTCAAATTCCGCTGTCCAGCTGCAGGGAACCCCATGCCTACCATCCACTGGCTCAAGGATGGACAGGC CTTCCACGGGGAGAATCGTATTGGAGGCATTCGGCTGCGCCACCAACACTGGAGCCTGGTGATGGAAAGT GTGGTACCCTCGGACCGTGGCACATACACATGCCTTGTGGAGAACTCTCTGGGTAGCATTCGCTACAGCT ATCTCCTGGATGTGCTGGAGCGGTCCCCGCACCGGCCCATCCTGCAGGCGGGGCTCCCAGCCAACACCAC AGCTGTGGTTGGCAGCGATGTGGAGCTACTCTGCAAGGTGTACAGCGACGCCCAGCCCCACATACAGTGG CTGAAACACGTCGTCATCAACGGCAGCAGCTTCGGCGCCGACGGTTTCCCCTACGTACAAGTCCTGAAGA CAACAGACATCAATAGCTCGGAGGTAGAGGTCTTGTATCTGAGGAACGTGTCCGCTGAGGATGCAGGAGA GTATACCTGTCTGGCGGGCAACTCCATCGGCCTTTCCTACCAGTCAGCGTGGCTCACGGTGCTGCCAGAG GAAGACCTCACGTGGACAACAGCAACCCCTGAGGCCAGATACACAGATATCATCCTGTATGTATCAGGCT CACTGGTTCTGCTTGTGCTCCTGCTGCTGGCCGGGGTGTATCATCGGCAAGTCATCCGTGGCCACTACTC TCGCCAGCCTGTCACTATACAAAAGCTGTCCCGTTTCCCTTTGGCCCGACAGTTCTCTTTGGAGTCGAGG TCCTCTGGCAAGTCAAGTTTGTCCCTGGTGCGAGGTGTCCGTCTCTCCTCCAGCGGCCCGCCCTTGCTCA CGGGCCTTGTGAATCTAGACCTGCCTCTCGATCCGCTTTGGGAATTCCCCCGGGACAGGTTGGTGCTCGG AAAGCCCCTGGGTGAGGGCTGCTTTGGGCAAGTGGTTCGTGCAGAGGCCTTTGGTATGGATCCCTCCCGG CCCGACCAAACCAGCACCGTGGCTGTGAAGATGCTGAAAGACAATGCCTCCGACAAGGATTTGGCAGACC TGGTCTCCGAGATGGAGGTGATGAAGCTAATCGGAAGACACAAGAACATCATCAACCTGCTGGGTGTCTG CACTCAGGAAGGGCCCCTGTACGTGATTGTGGAATGTGCCGCCAAGGGAAATCTTCGGGAATTCCTCCGT GCCCGGCGCCCCCCAGGCCCTGATCTCAGCCCTGATGGACCTCGGAGCAGCGAAGGACCACTCTCCTTCC CGGCCCTAGTCTCCTGTGCCTACCAGGTGGCCCGAGGCATGCAGTATCTGGAGTCTCGGAAGTGCATCCA CCGGGACCTGGCTGCCCGAAATGTGCTGGTGACCGAGGATGATGTGATGAAGATCGCTGACTTTGGGCTG GCACGTGGTGTCCACCACATTGACTACTATAAGAAAACCAGCAACGGCCGCCTGCCAGTCAAATGGATGG CTCCAGAGGCGTTGTTCGACCGTGTGTACACACACCAGAGTGACGTGTGGTCTTTCGGGATCCTGCTGTG GGAAATCTTCACCCTCGGGGGCTCCCCATACCCTGGCATTCCGGTGGAGGAGCTCTTCTCACTGCTGCGA GAGGGGCACAGGATGGAGCGGCCCCCAAACTGCCCCTCAGAGCTGTATGGGCTAATGAGGGAGTGCTGGC ACGCAGTCCCATCTCAGAGGCCTACTTTTAAGCAGCTGGTGGAAGCTCTGGACAAGGTCCTGCTGGCTGT CTCTGAAGAGTACCTTGACCTCCGCCTGACCTTTGGACCCTTTTCTCCCTCCAATGGGGATGCCAGCAGC ACCTGCTCCTCCAGTGACTCGGTTTTCAGCCACGACCCTTTGCCCCTCGAGCCAAGCCCCTTCCCTTTCT CTGACTCGCAGACGACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210769 representing NM_008011
Red=Cloning site Green=Tags(s) MWLLLALLSIFQGTPALSLEASEEMEQEPCLAPILEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRL ASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLLMDDSLTSISNDEDPKTLSSSSSGHVYP QQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKDGQAFHGENRIGGIRLRHQHWSLVMES VVPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQW LKHVVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPE EDLTWTTATPEARYTDIILYVSGSLVLLVLLLLAGVYHRQVIRGHYSRQPVTIQKLSRFPLARQFSLESR SSGKSSLSLVRGVRLSSSGPPLLTGLVNLDLPLDPLWEFPRDRLVLGKPLGEGCFGQVVRAEAFGMDPSR PDQTSTVAVKMLKDNASDKDLADLVSEMEVMKLIGRHKNIINLLGVCTQEGPLYVIVECAAKGNLREFLR ARRPPGPDLSPDGPRSSEGPLSFPALVSCAYQVARGMQYLESRKCIHRDLAARNVLVTEDDVMKIADFGL ARGVHHIDYYKKTSNGRLPVKWMAPEALFDRVYTHQSDVWSFGILLWEIFTLGGSPYPGIPVEELFSLLR EGHRMERPPNCPSELYGLMRECWHAVPSQRPTFKQLVEALDKVLLAVSEEYLDLRLTFGPFSPSNGDASS TCSSSDSVFSHDPLPLEPSPFPFSDSQTT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008011 |
| ORF Size | 2397 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008011.2, NP_032037.2 |
| RefSeq Size | 3146 bp |
| RefSeq ORF | 2400 bp |
| Locus ID | 14186 |
| UniProt ID | Q03142 |
| MW | 89 kDa |
| Gene Summary | Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Plays a role in postnatal lung development. May be involved in the development of skeletal muscle cell lineages.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221681 | Fgfr4 (untagged) - Mouse fibroblast growth factor receptor 4 (Fgfr4), (10ug) |
CNY 10,260.00 |
|
| MG210769 | Fgfr4 (tGFP-tagged) - Mouse fibroblast growth factor receptor 4 (Fgfr4) |
CNY 6,370.00 |
|
| MR210769L1 | Lenti ORF clone of Fgfr4 (Myc-DDK-tagged) - Mouse fibroblast growth factor receptor 4 (Fgfr4) |
CNY 7,700.00 |
|
| MR210769L2 | Lenti ORF clone of Fgfr4 (mGFP-tagged) - Mouse fibroblast growth factor receptor 4 (Fgfr4) |
CNY 11,176.00 |
|
| MR210769L3 | Lenti ORF clone of Fgfr4 (Myc-DDK-tagged) - Mouse fibroblast growth factor receptor 4 (Fgfr4) |
CNY 7,700.00 |
|
| MR210769L4 | Lenti ORF clone of Fgfr4 (mGFP-tagged) - Mouse fibroblast growth factor receptor 4 (Fgfr4) |
CNY 11,176.00 |
