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6430527G18Rik (BC057128) Mouse Tagged ORF Clone
CAT＃: MR210583
- TrueORF®
6430527G18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 6430527G18 gene (cDNA clone MGC:63318 IMAGE:6810092)
ORF Plasmid: tGFP
"BC057128" in other vectors (4)
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CNY 5,680.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6430527G18Rik; Eap1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210583 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGCGCAGGTGTCCTCGTCCCGAAGACAGTCTTGCTACCTGTGCGACCTGCCTCGCATGCCCT GGGCCATGATCTGGGACTTCTCCGAACCCGTATGCCGTGGTTGCGTCAATTACGAAGGCGCCGACCGCAT AGAATTCGTGATCGAGACAGCACGCCAGCTGAAGCGGGCTCACGGTTGCTTCCAAGACGGTCGCTCCCCG GGGCCGCCGCCGCCAGTCGGGGTCAAGACGGTGGCCTTGTCCGCCAAGGAAGCGGCAGCAGCAGCTGCCG CCGCACAGCAACAGCAGCAGCAACAACAGCAGCAACAGCAGCAGCTCAACCACGTCGATGGTTCCACCAA ACCTGCAGTGTTGGCCGCCCCGTCCGGTCTGGAGCGTTATGGCCTGAGCGCAGCGGCCGCCGCGGCTGCC GCCGCCGCTGCGGTGGAACAACGCAGCCGGTTCGAGTATCCGCCTCCTCCGGTGAGTCTGGGGAGCAGCA GCCACGCCGCCCGGCTGCCCAACGGCCTGGGAGGTCCTAACGGCTTCCCCAAGCCGGCACCAGAGGAAGG CCCCCCGGAGCTGAACCGCCAGAGCCCCAACTCATCCTCAGCAGCAACATCGGTGGCTTCTCGGCGTGGG ACGCATAGTGGGTTGGTGACCGGGCTGCCCAACCCGGGTGGTGGCGGGGGACCTCAGCTAACCGTGCCCC CCAACCTATTGCCGCAGACGCTGCTTAACGGCCCGGCCAGCGCCGCGGTGCTGCCTCCGCCCCACGGGTT GGGGGGAAGCCGAGGGCCCCCGACTCCAGCACCTCCGGGTGCTCCCGGGGGCCCCGCATGTCTCGGGGGT CCCCCGGGTGTGTCAGCCACTGTATCCTCCGCTCCATCATCGACTTCGTCGACGGTGGCAGAGGTGGGCG TGGGTGCTGCTGGCAAGAGGCCTGGATCGGTGTCGAGCACGGACCAGGAGCGTGAGCTAAAGGAGAAGCA GCGCAACGCTGAGGCCTTGGCAGAGCTGAGCGAGAGCCTGCGCAACCGCGCGGAGGAGTGGGCCAACAAG CCCAAGATGGTCCGCGACACGCTGCTCACGCTGGCTGGCTGCACGCCCTACGAGGTGCGCTTCAAGAAGG ACCACTCGCTTTTGGGTCGCGTCTTTGCCTTCGACGCGGTCTCTAAGCCGGGCATGGACTATGAGCTGAA GCTGTTCATTGAGTATCCCACGGGCTCCGGCAATGTGTACTCCAGCGCGTCTGGGGTGGCCAAACAGATG TACCAGGATTGCATGAAGGACTTTGGCCGGGGTCTGTCCTCTGGCTTCAAGTACCTGGAGTACGAGAAGA AGCACGGCTCTGGCGACTGGCGCCTACTTGGGGACCTACTGCCTGAGGCTGTGCGCTTCTTCAAGGAGGG CGTGCCCGGCGCGGACATGCTGCCCCAGCCCTACCTAGATGCTAGCTGTCCCATGCTACCCACGGCGCTG GTGAGTCTCAGCCGCGCCCCCAGCGCACCTCCAGGAACTGGAGCCTTGCCACCCGCGGCGCCCACGGGCC GGGGTGCAGCGTCCAGCCTGCGCAAAAGGAAGGCATCCCCGGAGCCTCCAGACTCAGCTGAAAGCGCACT GAAGCTCGGTGAGGAACAGCAGAGGCAGCAGTGGATGGCAAACCAGAGCGAGGCGCTGAAGCTCACGATG TCCGCGGGGGGCTTTGCGGCGCCGGGACACTCAGCAGGGGGACCACCTCCGCCCCCTCCACCTCTGGGAC CTCATTCCAATCGGACCACCCCGCCGGAGTCAGCCCCCCAGAACGGTCCGTCCCCCATGGCTGCCCTCAT GTCGGTAGCAGACACTTTGGGCACAGCTCATTCGCCCAAGGACGGCAGTTCAGTGCACTCGACCACTGCA TCTGCTAGGCGAAACAGCAGCAGCCCGGTGTCACCCGCCTCCGTGCCGGGCCAGCGTCGCCTGGCATCAC GCAATGGGGACCTGAATTTACAGGTGGCGCCCCCGCCGCCTAGCGCCCACCCGGGCATGGACCAAGTACA CCCCCAAAACATTCCGGATTCCCCTATGGCCAACAGCGGACCCCTCTGCTGTACCATTTGCCACGAACGT TTGGAGGACACGCATTTCGTTCAGTGTCCGTCTGTCCCTAGCCACAAATTTTGTTTCCCTTGTTCTAGAG AGAGTATCAAGGCCCAGGGGGCCACAGGTGAGGTGTATTGCCCCAGCGGAGAGAAATGCCCCCTAGTCGG GTCTAATGTACCTTGGGCCTTCATGCAGGGCGAAATTGCAACTATCTTAGCTGGGGATGTTAAAGTGAAA AAAGAGAGAGACCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210583 protein sequence
Red=Cloning site Green=Tags(s) MSAAQVSSSRRQSCYLCDLPRMPWAMIWDFSEPVCRGCVNYEGADRIEFVIETARQLKRAHGCFQDGRSP GPPPPVGVKTVALSAKEAAAAAAAAQQQQQQQQQQQQQLNHVDGSTKPAVLAAPSGLERYGLSAAAAAAA AAAAVEQRSRFEYPPPPVSLGSSSHAARLPNGLGGPNGFPKPAPEEGPPELNRQSPNSSSAATSVASRRG THSGLVTGLPNPGGGGGPQLTVPPNLLPQTLLNGPASAAVLPPPHGLGGSRGPPTPAPPGAPGGPACLGG PPGVSATVSSAPSSTSSTVAEVGVGAAGKRPGSVSSTDQERELKEKQRNAEALAELSESLRNRAEEWANK PKMVRDTLLTLAGCTPYEVRFKKDHSLLGRVFAFDAVSKPGMDYELKLFIEYPTGSGNVYSSASGVAKQM YQDCMKDFGRGLSSGFKYLEYEKKHGSGDWRLLGDLLPEAVRFFKEGVPGADMLPQPYLDASCPMLPTAL VSLSRAPSAPPGTGALPPAAPTGRGAASSLRKRKASPEPPDSAESALKLGEEQQRQQWMANQSEALKLTM SAGGFAAPGHSAGGPPPPPPPLGPHSNRTTPPESAPQNGPSPMAALMSVADTLGTAHSPKDGSSVHSTTA SARRNSSSPVSPASVPGQRRLASRNGDLNLQVAPPPPSAHPGMDQVHPQNIPDSPMANSGPLCCTICHER LEDTHFVQCPSVPSHKFCFPCSRESIKAQGATGEVYCPSGEKCPLVGSNVPWAFMQGEIATILAGDVKVK KERDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC057128 |
| ORF Size | 2325 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057128, AAH57128 |
| RefSeq Size | 3119 bp |
| RefSeq ORF | 2327 bp |
| Locus ID | 238330 |
| MW | 80.6 kDa |
| Gene Summary | Probable E3 ubiquitin protein ligase involved in the proteasome-mediated ubiquitin-dependent degradation of target proteins. Through the degradation of CTNNB1, functions downstream of FOXF2 to negatively regulate the Wnt signaling pathway. Probably plays a role in the development of the central nervous system and in neuronal maintenance (By similarity). Also acts as a transcriptional regulator of genes controlling female reproductive function. May play a role in gene transcription by transactivating GNRH1 promoter and repressing PENK promoter (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220922 | 6430527G18Rik (untagged) - Mouse RIKEN cDNA 6430527G18 gene (cDNA clone MGC:63318 IMAGE:6810092), (10ug) |
CNY 9,120.00 |
|
| MG210583 | 6430527G18Rik (tGFP-tagged) - Mouse RIKEN cDNA 6430527G18 gene (cDNA clone MGC:63318 IMAGE:6810092) |
CNY 4,940.00 |
|
| MR210583L3 | Lenti ORF clone of 6430527G18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 6430527G18 gene (cDNA clone MGC:63318 IMAGE:6810092) |
CNY 6,370.00 |
|
| MR210583L4 | Lenti ORF clone of 6430527G18Rik (mGFP-tagged) - Mouse RIKEN cDNA 6430527G18 gene (cDNA clone MGC:63318 IMAGE:6810092) |
CNY 6,370.00 |
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