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6430527G18Rik (BC057128) Mouse Untagged Clone
CAT＃: MC220922
6430527G18Rik (untagged) - Mouse RIKEN cDNA 6430527G18 gene (cDNA clone MGC:63318 IMAGE:6810092), (10ug)
CN¥ 9,120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 6430527G18Rik; Eap1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC057128
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCGGCGCAGGTGTCCTCGTCCCGAAGACAGTCTTGCTACCTGTGCGACCTGCCTCGCATGCCCT GGGCCATGATCTGGGACTTCTCCGAACCCGTATGCCGTGGTTGCGTCAATTACGAAGGCGCCGACCGCAT AGAATTCGTGATCGAGACAGCACGCCAGCTGAAGCGGGCTCACGGTTGCTTCCAAGACGGTCGCTCCCCG GGGCCGCCGCCGCCAGTCGGGGTCAAGACGGTGGCCTTGTCCGCCAAGGAAGCGGCAGCAGCAGCTGCCG CCGCACAGCAACAGCAGCAGCAACAACAGCAGCAACAGCAGCAGCTCAACCACGTCGATGGTTCCACCAA ACCTGCAGTGTTGGCCGCCCCGTCCGGTCTGGAGCGTTATGGCCTGAGCGCAGCGGCCGCCGCGGCTGCC GCCGCCGCTGCGGTGGAACAACGCAGCCGGTTCGAGTATCCGCCTCCTCCGGTGAGTCTGGGGAGCAGCA GCCACGCCGCCCGGCTGCCCAACGGCCTGGGAGGTCCTAACGGCTTCCCCAAGCCGGCACCAGAGGAAGG CCCCCCGGAGCTGAACCGCCAGAGCCCCAACTCATCCTCAGCAGCAACATCGGTGGCTTCTCGGCGTGGG ACGCATAGTGGGTTGGTGACCGGGCTGCCCAACCCGGGTGGTGGCGGGGGACCTCAGCTAACCGTGCCCC CCAACCTATTGCCGCAGACGCTGCTTAACGGCCCGGCCAGCGCCGCGGTGCTGCCTCCGCCCCACGGGTT GGGGGGAAGCCGAGGGCCCCCGACTCCAGCACCTCCGGGTGCTCCCGGGGGCCCCGCATGTCTCGGGGGT CCCCCGGGTGTGTCAGCCACTGTATCCTCCGCTCCATCATCGACTTCGTCGACGGTGGCAGAGGTGGGCG TGGGTGCTGCTGGCAAGAGGCCTGGATCGGTGTCGAGCACGGACCAGGAGCGTGAGCTAAAGGAGAAGCA GCGCAACGCTGAGGCCTTGGCAGAGCTGAGCGAGAGCCTGCGCAACCGCGCGGAGGAGTGGGCCAACAAG CCCAAGATGGTCCGCGACACGCTGCTCACGCTGGCTGGCTGCACGCCCTACGAGGTGCGCTTCAAGAAGG ACCACTCGCTTTTGGGTCGCGTCTTTGCCTTCGACGCGGTCTCTAAGCCGGGCATGGACTATGAGCTGAA GCTGTTCATTGAGTATCCCACGGGCTCCGGCAATGTGTACTCCAGCGCGTCTGGGGTGGCCAAACAGATG TACCAGGATTGCATGAAGGACTTTGGCCGGGGTCTGTCCTCTGGCTTCAAGTACCTGGAGTACGAGAAGA AGCACGGCTCTGGCGACTGGCGCCTACTTGGGGACCTACTGCCTGAGGCTGTGCGCTTCTTCAAGGAGGG CGTGCCCGGCGCGGACATGCTGCCCCAGCCCTACCTAGATGCTAGCTGTCCCATGCTACCCACGGCGCTG GTGAGTCTCAGCCGCGCCCCCAGCGCACCTCCAGGAACTGGAGCCTTGCCACCCGCGGCGCCCACGGGCC GGGGTGCAGCGTCCAGCCTGCGCAAAAGGAAGGCATCCCCGGAGCCTCCAGACTCAGCTGAAAGCGCACT GAAGCTCGGTGAGGAACAGCAGAGGCAGCAGTGGATGGCAAACCAGAGCGAGGCGCTGAAGCTCACGATG TCCGCGGGGGGCTTTGCGGCGCCGGGACACTCAGCAGGGGGACCACCTCCGCCCCCTCCACCTCTGGGAC CTCATTCCAATCGGACCACCCCGCCGGAGTCAGCCCCCCAGAACGGTCCGTCCCCCATGGCTGCCCTCAT GTCGGTAGCAGACACTTTGGGCACAGCTCATTCGCCCAAGGACGGCAGTTCAGTGCACTCGACCACTGCA TCTGCTAGGCGAAACAGCAGCAGCCCGGTGTCACCCGCCTCCGTGCCGGGCCAGCGTCGCCTGGCATCAC GCAATGGGGACCTGAATTTACAGGTGGCGCCCCCGCCGCCTAGCGCCCACCCGGGCATGGACCAAGTACA CCCCCAAAACATTCCGGATTCCCCTATGGCCAACAGCGGACCCCTCTGCTGTACCATTTGCCACGAACGT TTGGAGGACACGCATTTCGTTCAGTGTCCGTCTGTCCCTAGCCACAAATTTTGTTTCCCTTGTTCTAGAG AGAGTATCAAGGCCCAGGGGGCCACAGGTGAGGTGTATTGCCCCAGCGGAGAGAAATGCCCCCTAGTCGG GTCTAATGTACCTTGGGCCTTCATGCAGGGCGAAATTGCAACTATCTTAGCTGGGGATGTTAAAGTGAAA AAAGAGAGAGACCCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC057128 |
| Insert Size | 2328 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057128, AAH57128 |
| RefSeq Size | 3119 bp |
| RefSeq ORF | 2327 bp |
| Locus ID | 238330 |
| Gene Summary | Probable E3 ubiquitin protein ligase involved in the proteasome-mediated ubiquitin-dependent degradation of target proteins. Through the degradation of CTNNB1, functions downstream of FOXF2 to negatively regulate the Wnt signaling pathway. Probably plays a role in the development of the central nervous system and in neuronal maintenance (By similarity). Also acts as a transcriptional regulator of genes controlling female reproductive function. May play a role in gene transcription by transactivating GNRH1 promoter and repressing PENK promoter (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210583 | 6430527G18Rik (tGFP-tagged) - Mouse RIKEN cDNA 6430527G18 gene (cDNA clone MGC:63318 IMAGE:6810092) |
CN¥ 4,940.00 |
|
| MR210583 | 6430527G18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 6430527G18 gene (cDNA clone MGC:63318 IMAGE:6810092) |
CN¥ 5,680.00 |
|
| MR210583L3 | Lenti ORF clone of 6430527G18Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 6430527G18 gene (cDNA clone MGC:63318 IMAGE:6810092) |
CN¥ 6,370.00 |
|
| MR210583L4 | Lenti ORF clone of 6430527G18Rik (mGFP-tagged) - Mouse RIKEN cDNA 6430527G18 gene (cDNA clone MGC:63318 IMAGE:6810092) |
CN¥ 6,370.00 |
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