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Med25 (NM_029365) Mouse Tagged ORF Clone
CAT＃: MR210426
- TrueORF®
Med25 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) (Med25)
ORF Plasmid: tGFP
"NM_029365" in other vectors (4)
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CNY 5,464.00
CNY 6,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610034E13Rik; 2610529E18Rik; ESTM2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210426 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCCCGGATCCGAAGGCCCGGCGCGGGCCGGGGGCCTGGTCGCCGATGTGGTGTTTGTGATCGAGG GGACAGCAAACCTGGGGCCGTACTTCGAGGAGCTCCGCAAGCACTACCTGCTGCCTGCCATAGAGTACTT CAACGGGGGACCCCCTGCAGAGACGGACTTTGGGGGAGACTATGGTGGAACCCAGTACAGCCTTGTGGTG TTCAACACCGTGGACTGCGCTCCAGAGTCCTATGTACAATGTCACGCACCTACCAGCAGTGCCTATGAGT TTGTCACCTGGCTCGATGGCATCAAGTTCATGGGCGGTGGTGGCGAGAGCTGTAGTCTCATCGCTGAAGG TCTGAGCACTGCCCTGCAGCTGTTCGATGACTTTAAGAAGATGCGTGAACAGATTGGCCAGACCCACCGA GTCTGTCTCCTCATCTGCAACTCACCCCCGTACCTCCTGCCCGCCGTGGAGAGCACCACCTACTCAGGCT GCACTACCGAGAGCCTGGTGCAGAAAATAGGAGAGCGAGGCATCCACTTCTCCATCGTTTCTCCGAGAAA GCTGCCAGCCCTCCGGCTTCTGTTTGAGAAGGCGGCTCCTCCTGCCCTACTGGAGCCTTTGCAGCAACCA GCAGACGTCAGCCAGGACCCCAGGCACATGGTGCTTGTGCGTGGGCTCGTGCTGCCTGTTGGTGGCAGCT CGACCTCAGGCTCTCTGCAGACCAAGCAAGCAGTCCCCTTGCCACCAGCTCCAGCTTCTGCTGCCACGCT GTCAGCAGCGCCCCCGCAGGCTCTGCCCCCTGTCCCACCACAGTACCAGGTTCCTGGGAACCTAAGTGCT GCTCAGGTGGCTGCGCAGAATGCTGTGGAGGCTGCCAAGAGCCAGAAGGCCGGGCTGGGCCCACGCTTCT CACCCATCAACCCTCTCCAGCAAGCTGCTCCAGGAGTGGGGCCCCCCTTCAGCCAGGCCCCAGCACCCCC ATTGGCTCCTGTGCCCCCTGGAGCTCCAAAGCCACCTCCTGCCTCACAGCCTAGCCTAGTCTCCACCGTG GCCCCCGGACCAGTCCTGGCAGCTCCTGCGCAGCCTGGGGCGCCGTCTCTGGCAGGCACTGTAACCCCAG GTGGGGTCAATGGTCCTTCAGCAGCCCAGCTTGGGGGTCCTGCACTTGGTGGGCAGCAGTCAGTATCCAA CAAGCTCCTAGCCTGGAGTGGAGTCCTCGAGTGGCAGGAGAAACCCAAGCCTGCATCTGTGGATGCCAAC ACCAAGCTGACGAGGTCTCTGCCTTGCCAGGTCTATGTGAACCATGGAGAAAACCTGAAGACTGAGCAGT GGCCCCAGAAACTGATCATGCAGCTTATCCCTCAGCAGCTGCTGACCACCCTGGGTCCGCTGTTCCGGAA CTCAAGAATGGTTCAGTTCCACTTCACCAACAAGGACCTGGAGTCCCTCAAAGGCCTCTACCGCATCATG GGCAATGGCTTTGCGGGCTGCGTGCATTTCCCGCACACGGCCCCATGCGAGGTGCGCGTGCTTATGCTCC TGTACTCGTCTAAGAAGAAGATCTTCATGGGCCTCATCCCCTACGACCAGAGCGGCTTCGTCAATGGCAT CCGCCAGGTCATCACCAACCACAAGCAGGTCCAGCAGCAGAAGCTGGAGCAACAGCGCGGGATGGGGGCA CAGCAGGCACCTCCAGTCCTGGGCCCCATTCTGGAGGAGCAAGCGAGGCCCCCGCAGAATCTACTACAGC TCCGTGCACCACAGCCTCAGCCTCAGGGTGCCGTGGGAGCTTCTGCGGCTACAGGGCAGCCCCAGCCCCA AGGTGCTACCCAGGCCCCCACAGGCGCACCTCAAGGTCCTCCTGGAGCAGCCCCTGGCCCACCTCCTTCT GGACCCATCCTGCGGCCCCAGAACCCTGGGGCCAACCCCCAGCTGCGGAGCCTTCTCCTTAACCCAGCAC CGCCCCAGACTGGGGTGCCCCCGCCCCAGGCCTCCCTACACCACCTACAGCCTCCAGGGGCCCCTACATT GCTGCCACCACATCAGAGCATGGGGCAGCCCCAGCTGGGGCCGCAGCTCCTGCATCCTCCACCTGCCCAG TCATGGCCCACACAGCTTCCCCAGCGGGCGCCACTGCCAGGTCAGATGCTGCTGAGCGGGGGTCCCAGGG GCCCGGTCCCCCAGCCGGGCCTGCAGCCCAGCGTCATGGAGGACGACATCCTCATGGACCTCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210426 protein sequence
Red=Cloning site Green=Tags(s) MVPGSEGPARAGGLVADVVFVIEGTANLGPYFEELRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVV FNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHR VCLLICNSPPYLLPAVESTTYSGCTTESLVQKIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQQP ADVSQDPRHMVLVRGLVLPVGGSSTSGSLQTKQAVPLPPAPASAATLSAAPPQALPPVPPQYQVPGNLSA AQVAAQNAVEAAKSQKAGLGPRFSPINPLQQAAPGVGPPFSQAPAPPLAPVPPGAPKPPPASQPSLVSTV APGPVLAAPAQPGAPSLAGTVTPGGVNGPSAAQLGGPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDAN TKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIM GNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQRGMGA QQAPPVLGPILEEQARPPQNLLQLRAPQPQPQGAVGASAATGQPQPQGATQAPTGAPQGPPGAAPGPPPS GPILRPQNPGANPQLRSLLLNPAPPQTGVPPPQASLHHLQPPGAPTLLPPHQSMGQPQLGPQLLHPPPAQ SWPTQLPQRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029365 |
| ORF Size | 2238 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029365.3 |
| RefSeq Size | 2291 bp |
| RefSeq ORF | 2238 bp |
| Locus ID | 75613 |
| UniProt ID | Q8VCB2 |
| MW | 78.1 kDa |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201580 | Med25 (untagged) - Mouse mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) (Med25), (10ug) |
CNY 5,464.00 |
|
| MG210426 | Med25 (tGFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) (Med25) |
CNY 7,220.00 |
|
| MR210426L3 | Lenti ORF clone of Med25 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) (Med25) |
CNY 8,460.00 |
|
| MR210426L4 | Lenti ORF clone of Med25 (mGFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) (Med25) |
CNY 8,460.00 |
