United States
Germany
Japan
United Kingdom
China
Med25 (NM_029365) Mouse Untagged Clone
CAT＃: MC201580
Med25 (untagged) - Mouse mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) (Med25), (10ug)
CNY 5,464.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2610034E13Rik; 2610529E18Rik; ESTM2 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC021333 sequence for NM_029365
CCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGGCATGGTCCCCGGATCCGAAGGCCCGGCGCGGGCC GGGGGCCTGGTCGCCGATGTGGTGTTTGTGATCGAGGGGACAGCAAACCTGGGGCCGTACTTCGAGGAGC TCCGCAAGCACTACCTGCTGCCTGCCATAGAGTACTTCAACGGGGGACCCCCTGCAGAGACGGACTTTGG GGGAGACTATGGTGGAACCCAGTACAGCCTTGTGGTGTTCAACACCGTGGACTGCGCTCCAGAGTCCTAT GTACAATGTCACGCACCTACCAGCAGTGCCTATGAGTTTGTCACCTGGCTCGATGGCATCAAGTTCATGG GCGGTGGTGGCGAGAGCTGTAGTCTCATCGCTGAAGGTCTGAGCACTGCCCTGCAGCTGTTCGATGACTT TAAGAAGATGCGTGAACAGATTGGCCAGACCCACCGAGTCTGTCTCCTCATCTGCAACTCACCCCCGTAC CTCCTGCCCGCCGTGGAGAGCACCACCTACTCAGGCTGCACTACCGAGAGCCTGGTGCAGAAAATAGGAG AGCGAGGCATCCACTTCTCCATCGTTTCTCCGAGAAAGCTGCCAGCCCTCCGGCTTCTGTTTGAGAAGGC GGCTCCTCCTGCCCTACTGGAGCCTTTGCAGCAACCAGCAGACGTCAGCCAGGACCCCAGGCACATGGTG CTTGTGCGTGGGCTCGTGCTGCCTGTTGGTGGCAGCTCGACCTCAGGCTCTCTGCAGACCAAGCAAGCAG TCCCCTTGCCACCAGCTCCAGCTTCTGCTGCCACGCTGTCAGCAGCGCCCCCGCAGGCTCTGCCCCCTGT CCCACCACAGTACCAGGTTCCTGGGAACCTAAGTGCTGCTCAGGTGGCTGCGCAGAATGCTGTGGAGGCT GCCAAGAGCCAGAAGGCCGGGCTGGGCCCACGCTTCTCACCCATCAACCCTCTCCAGCAAGCTGCTCCAG GAGTGGGGCCCCCCTTCAGCCAGGCCCCAGCACCCCCATTGGCTCCTGTGCCCCCTGGAGCTCCAAAGCC ACCTCCTGCCTCACAGCCTAGCCTAGTCTCCACCGTGGCCCCCGGACCAGTCCTGGCAGCTCCTGCGCAG CCTGGGGCGCCGTCTCTGGCAGGCACTGTAACCCCAGGTGGGGTCAATGGTCCTTCAGCAGCCCAGCTTG GGGGTCCTGCACTTGGTGGGCAGCAGTCAGTATCCAACAAGCTCCTAGCCTGGAGTGGAGTCCTCGAGTG GCAGGAGAAACCCAAGCCTGCATCTGTGGATGCCAACACCAAGCTGACGAGGTCTCTGCCTTGCCAGGTC TATGTGAACCATGGAGAAAACCTGAAGACTGAGCAGTGGCCCCAGAAACTGATCATGCAGCTTATCCCTC AGCAGCTGCTGACCACCCTGGGTCCGCTGTTCCGGAACTCAAGAATGGTTCAGTTCCACTTCACCAACAA GGACCTGGAGTCCCTCAAAGGCCTCTACCGCATCATGGGCAATGGCTTTGCGGGCTGCGTGCATTTCCCG CACACGGCCCCATGCGAGGTGCGCGTGCTTATGCTCCTGTACTCGTCTAAGAAGAAGATCTTCATGGGCC TCATCCCCTACGACCAGAGCGGCTTCGTCAATGGCATCCGCCAGGTCATCACCAACCACAAGCAGGTCCA GCAGCAGAAGCTGGAGCAACAGCGCGGGATGGGGGCACAGCAGGCACCTCCAGTCCTGGGCCCCATTCTG GAGGAGCAAGCGAGGCCCCCGCAGAATCTACTACAGCTCCGTGCACCACAGCCTCAGCCTCAGGGTGCCG TGGGAGCTTCTGCGGCTACAGGGCAGCCCCAGCCCCAAGGTGCTACCCAGGCCCCCACAGGCGCACCTCA AGGTCCTCCTGGAGCAGCCCCTGGCCCACCTCCTTCTGGACCCATCCTGCGGCCCCAGAACCCTGGGGCC AACCCCCAGCTGCGGAGCCTTCTCCTTAACCCAGCACCGCCCCAGACTGGGGTGCCCCCGCCCCAGGCCT CCCTACACCACCTACAGCCTCCAGGGGCCCCTACATTGCTGCCACCACATCAGAGCATGGGGCAGCCCCA GCTGGGGCCGCAGCTCCTGCATCCTCCACCTGCCCAGTCATGGCCCACACAGCTTCCCCAGCGGGCGCCA CTGCCAGGTCAGATGCTGCTGAGCGGGGGTCCCAGGGGCCCGGTCCCCCAGCCGGGCCTGCAGCCCAGCG TCATGGAGGACGACATCCTCATGGACCTCATCTGAACCCAACACCCAATAAAGTTCCTTTTAACAAAAAA AAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_029365 |
| Insert Size | 2238 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC021333, AAH21333 |
| RefSeq Size | 2326 bp |
| RefSeq ORF | 2238 bp |
| Locus ID | 75613 |
| UniProt ID | Q8VCB2 |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Mediator complex subunit MED25 is targeted by the N-terminal transactivation domain of the PEA3 group members
,Alexis Verger, Jean-Luc Baert, Kathye Verreman, Frédérique Dewitte, Elisabeth Ferreira, Zoé Lens, Yvan de Launoit, Vincent Villeret, and Didier Monté,
Nucleic Acids Res., Mar 2013; 10.1093/nar/gkt199.
[MED25]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210426 | Med25 (tGFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) (Med25) |
CNY 7,220.00 |
|
| MR210426 | Med25 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) (Med25) |
CNY 5,464.00 |
|
| MR210426L3 | Lenti ORF clone of Med25 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) (Med25) |
CNY 8,460.00 |
|
| MR210426L4 | Lenti ORF clone of Med25 (mGFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 25 homolog (yeast) (Med25) |
CNY 8,460.00 |
