Thoc1 (NM_153552) Mouse Tagged ORF Clone
CAT#: MR209815
- TrueORF®
Thoc1 (Myc-DDK-tagged) - Mouse THO complex 1 (Thoc1)
ORF Plasmid: tGFP
"NM_153552" in other vectors (4)
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CNY 6,560.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 3110002N20Rik; AW107452; NMP-84 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209815 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCCGACGCCGGCGCTTTTCAGTTTGCCGGAGGCGCGGACGCGATTTACGAAATCTACCAGAGAAG CCTTGAATAACAAAAATATCAAGCCGTTGTTGACTGCCTTCAGTCAGTTACCTGGCAGTGAAAATGAAAA AAAATGTACCCTTGACCAAGCTTTCAGAGGTGTTCTGGAAGAAGAAATTATAAATCATTCCGCATGTGAG AACGTTTTAGCTATTATTTCTCTTGCTATTGGGGGAGTGACCGAAAGTGTTTGTACTGCGTCCACACCAT TTGTGCTATTGGGAGATGTCTTAGACTGCCTTCCTTTGGATCAGTGTGACACAATATTCACTTTTGTTGA AAAAAATGTTGCTACTTGGAAGTCAAATACATTCTATTCTGCTGGCAAAAATTATTTACTGCGTATGTGC AATGATCTCCTCAGAAGATTGTCTAAATCTCAGAATACAGTTTTCTGTGGAAGAATTCAGCTCTTTTTGG CCAGGCTTTTTCCTCTATCTGAGAAATCAGGTCTTAATTTGCAGAGTCAGTTTAACCTGGAAAACGTCAC TGTTTTCAATACAAACGAGCAGGAGAGCACCCTGGGTCAGAAGCATACCGAGGATAGAGAGGAAGGGATG GATGTAGAAGAAGGTGAAATGGGAGATGATGAAGCCCCGACAACTTGTTCTATTCCAATTGATTACAACC TGTATAGAAAATTCTGGTCACTTCAGGATTACTTCAGGAACCCAGTACAATGCTATGAGAAGATTTCTTG GAAAACGTTTCTCAAGTATTCTGAAGAAGTTTTAGCTGTTTTTAAAAGTTATAAATTAGATGATACTCAA GCCTCCAGGAAAAAGATGGAAGAATTGAAAACAGGAGGAGAGCATGTATACTTCGCCAAGTTTCTAACGA GTGAAAAGTTGATGGATTTACAACTGAGTGACAGTAACTTTCGTCGACACATCCTGTTGCAGTATCTCAT TTTATTTCAATATCTCAAGGGACAGGTCAAATTCAAAAGTTCAAATTATGTTTTAACTGATGAGCAATCA CTCTGGATTGAAGATACTACAAAATCAGTCTACCAACTGCTATCTGAAAATCCCCCTGATGGAGAAAGAT TTTCAAAGATGGTAGAGCATATATTAAACACTGAGGAAAATTGGAACTCATGGAAGAATGAAGGTTGCCC AAGTTTTGTTAAAGAAAGAGCGTCAGATACCAAGCCCACAAGAGTGGTGAGGAAGAGAGCAGCCCCAGAA GACTTCTTAGGCAAAGGGCCCAACAAGAAAATCCTCATTGGAAATGAGGAGCTCACAAGGCTTTGGAATC TCTGTCCTGATAATATGGAAGCCTGTAAATCAGAGACAAGAGAATACATGCCAACTTTGGAGGAGTTCTT TGAAGAAGCTATTGAACAAGCAGATCCTGAAAACATGGTTGAAAGTGAATATAAAGCCGTAAATAATTCA AATTATGGATGGAGAGCTCTTAGACTCTTAGCCCGGAGAAGCCCTCACTTCTTTCAGCCAACCAACCAGC AGTTTAAGAGTCTACCAGAGTATCTGGAAAACATGGTGATAAAGCTGGCCAAGGAACTACCACCCCCTTC TGAAGAAATAAAGACAGGTGAGGATGAAGACGAGGAAGATAATGATGCTTTACTAAAGGAAAATGAAAGT CCAGATGTTCGGCGAGATAAACCTATAACAGGGGAGCAGATAGAGTCATTTGCCAACAAACTTGGTGAGC AGTGGAAGATTTTGGCTCCTTACCTGGAAATTAAAGACTCTGATATTAGGCAGATTGAGTGTGACAGTGA AGACATGAAGATGAGAGCCAAGCAGCTCCTTGTTGCCTGGCAAGATCAAGAAGGAGTTCATGCAACAACT GATAACCTGATTAGTGCACTAAACAAGTCTGGATTAAGTGACCTTGCAGAAAGTCTAACTAATGACACTG AAACAAACAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209815 protein sequence
Red=Cloning site Green=Tags(s) MSPTPALFSLPEARTRFTKSTREALNNKNIKPLLTAFSQLPGSENEKKCTLDQAFRGVLEEEIINHSACE NVLAIISLAIGGVTESVCTASTPFVLLGDVLDCLPLDQCDTIFTFVEKNVATWKSNTFYSAGKNYLLRMC NDLLRRLSKSQNTVFCGRIQLFLARLFPLSEKSGLNLQSQFNLENVTVFNTNEQESTLGQKHTEDREEGM DVEEGEMGDDEAPTTCSIPIDYNLYRKFWSLQDYFRNPVQCYEKISWKTFLKYSEEVLAVFKSYKLDDTQ ASRKKMEELKTGGEHVYFAKFLTSEKLMDLQLSDSNFRRHILLQYLILFQYLKGQVKFKSSNYVLTDEQS LWIEDTTKSVYQLLSENPPDGERFSKMVEHILNTEENWNSWKNEGCPSFVKERASDTKPTRVVRKRAAPE DFLGKGPNKKILIGNEELTRLWNLCPDNMEACKSETREYMPTLEEFFEEAIEQADPENMVESEYKAVNNS NYGWRALRLLARRSPHFFQPTNQQFKSLPEYLENMVIKLAKELPPPSEEIKTGEDEDEEDNDALLKENES PDVRRDKPITGEQIESFANKLGEQWKILAPYLEIKDSDIRQIECDSEDMKMRAKQLLVAWQDQEGVHATT DNLISALNKSGLSDLAESLTNDTETNS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153552 |
ORF Size | 1974 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153552.1 |
RefSeq Size | 3855 bp |
RefSeq ORF | 1974 bp |
Locus ID | 225160 |
UniProt ID | Q8R3N6 |
MW | 75.4 kDa |
Gene Summary | Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220201 | Thoc1 (untagged) - Mouse THO complex 1 (Thoc1), (10ug) |
CNY 8,460.00 |
|
MG209815 | Thoc1 (tGFP-tagged) - Mouse THO complex 1 (Thoc1) |
CNY 7,220.00 |
|
MR209815L3 | Lenti ORF clone of Thoc1 (Myc-DDK-tagged) - Mouse THO complex 1 (Thoc1) |
CNY 8,460.00 |
|
MR209815L4 | Lenti ORF clone of Thoc1 (mGFP-tagged) - Mouse THO complex 1 (Thoc1) |
CNY 8,460.00 |