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Ndst3 (BC079622) Mouse Tagged ORF Clone
CAT＃: MR209708
- TrueORF®
Ndst3 (Myc-DDK-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (cDNA clone MGC:90637 IMAGE:30535990)
ORF Plasmid: tGFP
"BC079622" in other vectors (3)
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CNY 4,784.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4921531K01Rik; 4930511P15Rik; N-HSST 3; NDST-3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209708 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTTTATCATGAAACCTCACAGACACTTTCAGAGAACACTGATTCTGCTTGCCACCTTTTGTATGG TAAGCATTATTATTTCTGCTTACTACCTGTACAGCGGCTACAAACAGGAAAGTGAAGTCTCCGGGCGGGC TTCGGAAGTGGACTGTGGTGACCTCCAGCACATACCATCCAGGCTGATGGAAGTGAGGAGGACAATGATT TCCGATGCTTCAAGGACAGACCCCACAGTCCTGGTGTTTGTGGAGAGCCAGTACTCATCCCTTGGTCAAG ACATCATTATGATGCTAGAATCCATCCGGTTCCATTATCACACTGAAATCGCTCCTGGAAAAGGAGATCT TCCGGCACTTACAGACAATGTGAAGGGCAAATATGTTCTCATTATATATGAGAATATTCTAAAGTATATA AACATGGACTCTTGGAATAGAAGCCTTTTAGATAAATACTGTATAGAGTATGGTGTGGGTATCATTGGAT TCCATAAAACCAGTGAGAAAAATCTACAGAGCTTTCAGTTCAGGGGCTTCCCCTTTTCCATAAGTGGAAA CCTGGCAGTAAAAGATTGCTGTATTAATCCTCACTCCCCACTCCTTCGTGTGACCAAATCATCCAAGCTG GACAGAGGTTCTCTACCTGGAACTGACTGGACAGTTTTTCAGATTAACCACTCCACCTACCAGCCAGTAA TATTTGCCAAAGTTAAGACTCCAGAAAACCTTTCTCCTCCCATCTCTAAACATGCATTTTATGCCACTAT CATACACGACCTGGGGCTTCATGACGGGATCCAGCGAGTTCTTTTTGGCAACAACTTGAACTTCTGGCTA CACAAGCTCATCTTCATAGACGCCATCTCCTTCTTGTCGGGGAAGAGGCTGACACTGTCCTTGGACAGGT ACATCCTTGTGGACATTGACGACATATTTGTAGGGAAGGAGGGCACAAGGATGAACACCAATGATGTGAA GGCCCTGCTTGACACTCAGAATCTTTTGCGCACACAAATCACAAATTTTACATTCAACCTTGGATTTTCA GGGAAATTTTATCACACAGGAACTGAAGAGGAAGATGAAGGGGATGATTGTCTGCTGGGTTCTGTGGACG AGTTCTGGTGGTTTCCTCACATGTGGAGTCACATGCAGCCCCACCTCTTCCACAATGAGTCGTCTTTAAT AGAGCAGATGATTCTCAACAAAAAGTTTGCCTTAGAGCACGGCATCCCTACCGATATGGGATATGCAGTG TCCCCTCACCATTCAGGTGTCTACCCTGTCCATGTTCAGCTTTATGAGGCCTGGAAGAAGGTTTGGAATA TTAAAATCACCAGCACTGAAGAATATCCACATCTGAAACCAGCTAGATATCGGAGAGGCTTCATTCACAA AAACATCATGGTTCTCCCAAGACAAACCTGTGGGCTCTTCACCCACACAATTTTCTACAAGGAGTATCCA GGGGGTCCCAGGGAACTGGACAAGAGTATTCATGGAGGGGAGCTCTTCTTCACTGTGGTTCTCAACCCAA TCAGTATTTTCATGACCCATTTGTCTAACTATGGAAACGACCGACTGGGATTATATACATTTGTGAATCT AGCCAATTTTGTGCAGACCTGGACCAACCTGCGTCTTCAGACCTTGCCTCCAGCTCAGCTGGCTCACAAG TATTTTGAGCTCTTTCCTGATCAGAAAGACCCTCTCTGGCAGAACCCCTGTGATGACAAACGCCACAGAG ACATTTGGTCTAAAGAGAAAACCTGCGATCGTTTACCAAAATTCTTGGTAATAGGACCCCAGAAAACTGG TACCACTGCCTTGTGTCTGTTCCTGATTATGCATCCTTCCATCCTTAGTAACTCCCCCAGCCCAAAATCC TTTGAGGAGGTACATGGATTTCTTCCCAGTCCCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209708 protein sequence
Red=Cloning site Green=Tags(s) MSFIMKPHRHFQRTLILLATFCMVSIIISAYYLYSGYKQESEVSGRASEVDCGDLQHIPSRLMEVRRTMI SDASRTDPTVLVFVESQYSSLGQDIIMMLESIRFHYHTEIAPGKGDLPALTDNVKGKYVLIIYENILKYI NMDSWNRSLLDKYCIEYGVGIIGFHKTSEKNLQSFQFRGFPFSISGNLAVKDCCINPHSPLLRVTKSSKL DRGSLPGTDWTVFQINHSTYQPVIFAKVKTPENLSPPISKHAFYATIIHDLGLHDGIQRVLFGNNLNFWL HKLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNTNDVKALLDTQNLLRTQITNFTFNLGFS GKFYHTGTEEEDEGDDCLLGSVDEFWWFPHMWSHMQPHLFHNESSLIEQMILNKKFALEHGIPTDMGYAV SPHHSGVYPVHVQLYEAWKKVWNIKITSTEEYPHLKPARYRRGFIHKNIMVLPRQTCGLFTHTIFYKEYP GGPRELDKSIHGGELFFTVVLNPISIFMTHLSNYGNDRLGLYTFVNLANFVQTWTNLRLQTLPPAQLAHK YFELFPDQKDPLWQNPCDDKRHRDIWSKEKTCDRLPKFLVIGPQKTGTTALCLFLIMHPSILSNSPSPKS FEEVHGFLPSPI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC079622 |
| ORF Size | 1926 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC079622, AAH79622 |
| RefSeq Size | 5317 bp |
| RefSeq ORF | 1928 bp |
| Locus ID | 83398 |
| MW | 73.8 kDa |
| Gene Summary | Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217933 | Ndst3 (untagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (cDNA clone MGC:90637 IMAGE:30535990),, (10ug) |
CNY 6,940.00 |
|
| MG209708 | Ndst3 (tGFP-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (cDNA clone MGC:90637 IMAGE:30535990) |
CNY 4,090.00 |
|
| MR209708L3 | Lenti ORF clone of Ndst3 (Myc-DDK-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (cDNA clone MGC:90637 IMAGE:30535990) |
CNY 5,610.00 |
