Ndst3 (BC079622) Mouse Untagged Clone

CAT#: MC217933

Ndst3 (untagged) - Mouse N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (cDNA clone MGC:90637 IMAGE:30535990),, (10ug)



  "BC079622" in other vectors (3)

CNY 6,940.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4921531K01Rik; 4930511P15Rik; N-HSST 3; NDST-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC079622
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTTTATCATGAAACCTCACAGACACTTTCAGAGAACACTGATTCTGCTTGCCACCTTTTGTATGG
TAAGCATTATTATTTCTGCTTACTACCTGTACAGCGGCTACAAACAGGAAAGTGAAGTCTCCGGGCGGGC
TTCGGAAGTGGACTGTGGTGACCTCCAGCACATACCATCCAGGCTGATGGAAGTGAGGAGGACAATGATT
TCCGATGCTTCAAGGACAGACCCCACAGTCCTGGTGTTTGTGGAGAGCCAGTACTCATCCCTTGGTCAAG
ACATCATTATGATGCTAGAATCCATCCGGTTCCATTATCACACTGAAATCGCTCCTGGAAAAGGAGATCT
TCCGGCACTTACAGACAATGTGAAGGGCAAATATGTTCTCATTATATATGAGAATATTCTAAAGTATATA
AACATGGACTCTTGGAATAGAAGCCTTTTAGATAAATACTGTATAGAGTATGGTGTGGGTATCATTGGAT
TCCATAAAACCAGTGAGAAAAATCTACAGAGCTTTCAGTTCAGGGGCTTCCCCTTTTCCATAAGTGGAAA
CCTGGCAGTAAAAGATTGCTGTATTAATCCTCACTCCCCACTCCTTCGTGTGACCAAATCATCCAAGCTG
GACAGAGGTTCTCTACCTGGAACTGACTGGACAGTTTTTCAGATTAACCACTCCACCTACCAGCCAGTAA
TATTTGCCAAAGTTAAGACTCCAGAAAACCTTTCTCCTCCCATCTCTAAACATGCATTTTATGCCACTAT
CATACACGACCTGGGGCTTCATGACGGGATCCAGCGAGTTCTTTTTGGCAACAACTTGAACTTCTGGCTA
CACAAGCTCATCTTCATAGACGCCATCTCCTTCTTGTCGGGGAAGAGGCTGACACTGTCCTTGGACAGGT
ACATCCTTGTGGACATTGACGACATATTTGTAGGGAAGGAGGGCACAAGGATGAACACCAATGATGTGAA
GGCCCTGCTTGACACTCAGAATCTTTTGCGCACACAAATCACAAATTTTACATTCAACCTTGGATTTTCA
GGGAAATTTTATCACACAGGAACTGAAGAGGAAGATGAAGGGGATGATTGTCTGCTGGGTTCTGTGGACG
AGTTCTGGTGGTTTCCTCACATGTGGAGTCACATGCAGCCCCACCTCTTCCACAATGAGTCGTCTTTAAT
AGAGCAGATGATTCTCAACAAAAAGTTTGCCTTAGAGCACGGCATCCCTACCGATATGGGATATGCAGTG
TCCCCTCACCATTCAGGTGTCTACCCTGTCCATGTTCAGCTTTATGAGGCCTGGAAGAAGGTTTGGAATA
TTAAAATCACCAGCACTGAAGAATATCCACATCTGAAACCAGCTAGATATCGGAGAGGCTTCATTCACAA
AAACATCATGGTTCTCCCAAGACAAACCTGTGGGCTCTTCACCCACACAATTTTCTACAAGGAGTATCCA
GGGGGTCCCAGGGAACTGGACAAGAGTATTCATGGAGGGGAGCTCTTCTTCACTGTGGTTCTCAACCCAA
TCAGTATTTTCATGACCCATTTGTCTAACTATGGAAACGACCGACTGGGATTATATACATTTGTGAATCT
AGCCAATTTTGTGCAGACCTGGACCAACCTGCGTCTTCAGACCTTGCCTCCAGCTCAGCTGGCTCACAAG
TATTTTGAGCTCTTTCCTGATCAGAAAGACCCTCTCTGGCAGAACCCCTGTGATGACAAACGCCACAGAG
ACATTTGGTCTAAAGAGAAAACCTGCGATCGTTTACCAAAATTCTTGGTAATAGGACCCCAGAAAACTGG
TACCACTGCCTTGTGTCTGTTCCTGATTATGCATCCTTCCATCCTTAGTAACTCCCCCAGCCCAAAATCC
TTTGAGGAGGTACATGGATTTCTTCCCAGTCCCATCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC079622
Insert Size 1929 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC079622, AAH79622
RefSeq Size 5317 bp
RefSeq ORF 1928 bp
Locus ID 83398
Gene Summary Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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