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Trim9 (BC052034) Mouse Tagged ORF Clone
CAT＃: MR208585
- TrueORF®
Trim9 (Myc-DDK-tagged) - Mouse tripartite motif protein 9 (cDNA clone MGC:62392 IMAGE:5684877)
ORF Plasmid: tGFP
"BC052034" in other vectors (4)
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CN¥ 3,992.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208585 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAGATGGAAGAAGAGTTAAAATGCCCTGTGTGTGGCTCCTTCTATCGGGAGCCCATCATCTTGC CCTGCTCTCACAATTTATGTCAGGCGTGCGCCCGCAACATCCTGGTGCAGACCCCGGAGTCCGAGTCCCC CCAGAGCCGCCGGGCCTCGGGCTCTGGGGTTTCTGACTATGATTATCTGGACCTGGACAAGATGAGCCTG TACAGCGAGGCGGACAGCGGCTATGGCTCCTACGGAGGTTTCGCCAGCGCCCCCACTACCCCGTGCCAGA AGTCGCCCAACGGCGTCCGCGTTTTCCCCCCTGCTATGCCGCCACCGCCCACCCACCTGTCACCGGCTTT GGCCCCTGTGCCCCGCAATTCCTGCATCACCTGCCCCCAGTGCCACCGCAGCCTCATTCTGGATGACCGG GGGCTCCGCGGTTTCCCCAAGAACCGCGTCCTGGAAGGGGTCATCGACCGCTACCAACAGAGCAAAGCCG CGGCCCTCAAGTGCCAGCTCTGCGAGAAGGCGCCCAAGGAAGCCACGGTCATGTGTGAACAGTGCGATGT CTTCTACTGCGACCCTTGCCGTCTGCGCTGCCACCCGCCCCGGGGTCCCCTAGCCAAACACCGTCTGGTG CCCCCGGCCCAGGGCCGCGTCAGCCGGCGGCTGAGCCCGCGCAAGGTCTCCACCTGCACAGACCACGAGC TGGAGAACCACAGCATGTACTGCGTGCAATGCAAGATGCCCGTTTGCTACCAGTGCCTAGAGGAGGGCAA ACACTCCAGCCACGAAGTCAAGGCTTTGGGGGCTATGTGGAAATTGCACAAGAGCCAGCTCTCCCAGGCC CTGAATGGATTGTCGGACAGGGCCAAAGAAGCCAAGGAGTTCCTGGTGCAGCTCCGCACCATGGTACAAC AGATCCAGGAAAACAGTGTGGAGTTTGAGGCCTGCCTGGTGGCCCAGTGTGATGCGCTCATCGATGCCCT AAACCGAAGGAAGGCTCAGCTGCTGGCCCGGGTCAACAAGGAGCATGAACACAAGCTGAAGGTGGTTCGG GACCAGATCTCTCACTGCACGGTGAAGCTGCGTCAGACCACGGGCCTCATGGAGTACTGCTTGGAAGTGA TTAAGGAGAACGACCCCAGTGGCTTTTTGCAGATTTCAGATGCTCTCATAAGGCGAGTTCACTTAACTGA GGACCAGTGGGGGAAGGGCACCCTCACTCCCAGGATGACCACAGACTTTGATCTGAGCCTGGACAACAGC CCTCTGCTGCAGTCTATTCACCAACTGGACTTCGTGCAAGTGAAAGCCTCCTCTCCAGTCCCAGCAACCC CCATCCTACAGCTGGAGGAGTGTTGCACCCACAACAACAGTGCTACGCTGTCCTGGAAACAGCCCCCTCT GTCCACCGTAGCCGCCGATGGATACATCCTGGAGCTGGATGACGGCAGTGGTGGTCAGTTCCGGGAAGTG TATGTTGGAAAAGAAACAATGTGCACAGTGGACGGCCTTCACTTCAACAGCACATACAACGCTCGGGTTA AGGCCTTCAACAAAACAGGAGTCAGCCCCTACAGCAAGACACTGGTCCTCCAGACGTCTGAGGGAAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208585 protein sequence
Red=Cloning site Green=Tags(s) MEEMEEELKCPVCGSFYREPIILPCSHNLCQACARNILVQTPESESPQSRRASGSGVSDYDYLDLDKMSL YSEADSGYGSYGGFASAPTTPCQKSPNGVRVFPPAMPPPPTHLSPALAPVPRNSCITCPQCHRSLILDDR GLRGFPKNRVLEGVIDRYQQSKAAALKCQLCEKAPKEATVMCEQCDVFYCDPCRLRCHPPRGPLAKHRLV PPAQGRVSRRLSPRKVSTCTDHELENHSMYCVQCKMPVCYQCLEEGKHSSHEVKALGAMWKLHKSQLSQA LNGLSDRAKEAKEFLVQLRTMVQQIQENSVEFEACLVAQCDALIDALNRRKAQLLARVNKEHEHKLKVVR DQISHCTVKLRQTTGLMEYCLEVIKENDPSGFLQISDALIRRVHLTEDQWGKGTLTPRMTTDFDLSLDNS PLLQSIHQLDFVQVKASSPVPATPILQLEECCTHNNSATLSWKQPPLSTVAADGYILELDDGSGGQFREV YVGKETMCTVDGLHFNSTYNARVKAFNKTGVSPYSKTLVLQTSEGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC052034 |
| ORF Size | 1608 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052034, AAH52034 |
| RefSeq Size | 2413 bp |
| RefSeq ORF | 1610 bp |
| Locus ID | 94090 |
| MW | 59.7 kDa |
| Gene Summary | E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218512 | Trim9 (untagged) - Mouse tripartite motif protein 9 (cDNA clone MGC:62392 IMAGE:5684877), (10ug) |
CN¥ 6,940.00 |
|
| MG208585 | Trim9 (tGFP-tagged) - Mouse tripartite motif protein 9 (cDNA clone MGC:62392 IMAGE:5684877) |
CN¥ 3,330.00 |
|
| MR208585L3 | Lenti ORF clone of Trim9 (Myc-DDK-tagged) - Mouse tripartite motif protein 9 (cDNA clone MGC:62392 IMAGE:5684877) |
CN¥ 4,940.00 |
|
| MR208585L4 | Lenti ORF clone of Trim9 (mGFP-tagged) - Mouse tripartite motif protein 9 (cDNA clone MGC:62392 IMAGE:5684877) |
CN¥ 4,940.00 |
