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Coro1c (NM_011779) Mouse Tagged ORF Clone
CAT＃: MR207591
- TrueORF®
Coro1c (Myc-DDK-tagged) - Mouse coronin, actin binding protein 1C (Coro1c)
ORF Plasmid: tGFP
"NM_011779" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AL022675; AW455561; AW548837 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207591 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCGAGTGGTGCGGCAGAGCAAGTTCCGCCACGTCTTTGGACAGGCGGTGAAGAATGACCAGTGCT ATGACGACATCCGTGTCTCCCGAGTCACCTGGGACAGTTCCTTTTGTGCCGTCAACCCCAGATTCGTGGC CATCATCATAGAAGCGAGTGGGGGAGGGGCCTTCCTGGTGCTCCCTCTGCACAAGACGGGACGAATTGAC AAATCCTACCCTACAGTGTGTGGCCACACAGGACCAGTGCTGGACATAGACTGGTGTCCACATAATGACC AGGTCATTGCCAGCGGCTCAGAGGACTGCACTGTTATGGTTTGGCAGATCCCGGAAAATGGACTCACCCT TTCCCTGACCGAGCCTGTGGTGATTCTAGAAGGGCACTCAAAAAGAGTTGGCATCGTGGCCTGGCACCCG ACGGCCCGCAATGTGCTTCTCAGTGCAGGCTGTGATAATGCCATTATCATCTGGAACGTGGGGACAGGGG AAGCCCTCATCAACTTGGACGATATGCATTCAGACATGATTTACAATGTGAGCTGGAGCCGTAACGGTAG TCTCATCTGCACCGCATCCAAAGACAAGAAAGTGAGAGTCATTGACCCCAGGAAACAGGAGATTGTGGCG GAGAAGGAGAAAGCACACGAAGGAGCCAGGCCTATGCGTGCCATCTTCCTGGCGGACGGCAACGTCTTCA CTACTGGCTTTAGCCGCATGAGCGAGCGGCAGTTGGCGCTCTGGAACCCAAAAAACATGCAGGAACCAAT TGCTCTCCATGAAATGGACACTAGCAATGGGGTGCTGCTCCCCTTCTACGATCCGGACACCAGCATCATC TACTTATGTGGGAAGGGTGACAGCAGTATCCGCTATTTTGAGATCACGGATGAATCCCCATATGTCCACT ACCTCAACACATTCAGCAGCAAGGAGCCTCAGCGAGGGATGGGCTACATGCCCAAGCGGGGCCTTGACGT CAACAAGTGTGAAATTGCCAGATTCTTCAAGCTTCATGAGAGAAAGTGCGAGCCCATTATCATGACGGTC CCCAGGAAGTCTGACCTCTTCCAAGACGACCTGTACCCTGACACGGCAGGGCCGGAAGCCGCCCTTGAGG CCGAGGAGTGGTTTGAGGGGAAGAACGCAGACCCCATCCTCATATCGTTGAAGCACGGGTACATTCCAGG CAAGAACAGGGACCTCAAGGTGGTCAAGAAGAACATCCTGGACAGCAAGCCGGCTGCAAACAAGAAGAGC GAGCTCAGCTGTGCCCCCAAGAAGCCCACAGACACGGCCAGTGTGCAAAACGAAGCCAAGTTGGATGAGA TTCTGAAAGAGATCAAATCCATAAAGGAAACAATCTGCAGTCAAGACGAGCGCATTTCCAAGTTGGAGCA GCAGCTGGCGAAGATGGCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207591 protein sequence
Red=Cloning site Green=Tags(s) MRRVVRQSKFRHVFGQAVKNDQCYDDIRVSRVTWDSSFCAVNPRFVAIIIEASGGGAFLVLPLHKTGRID KSYPTVCGHTGPVLDIDWCPHNDQVIASGSEDCTVMVWQIPENGLTLSLTEPVVILEGHSKRVGIVAWHP TARNVLLSAGCDNAIIIWNVGTGEALINLDDMHSDMIYNVSWSRNGSLICTASKDKKVRVIDPRKQEIVA EKEKAHEGARPMRAIFLADGNVFTTGFSRMSERQLALWNPKNMQEPIALHEMDTSNGVLLPFYDPDTSII YLCGKGDSSIRYFEITDESPYVHYLNTFSSKEPQRGMGYMPKRGLDVNKCEIARFFKLHERKCEPIIMTV PRKSDLFQDDLYPDTAGPEAALEAEEWFEGKNADPILISLKHGYIPGKNRDLKVVKKNILDSKPAANKKS ELSCAPKKPTDTASVQNEAKLDEILKEIKSIKETICSQDERISKLEQQLAKMAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011779 |
| ORF Size | 1425 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011779.1, NM_011779.2, NM_011779.3, NP_035909.2 |
| RefSeq Size | 3436 bp |
| RefSeq ORF | 1425 bp |
| Locus ID | 23790 |
| UniProt ID | Q9WUM4 |
| MW | 53.1 kDa |
| Gene Summary | Plays a role in directed cell migration by regulating the activation and subcellular location of RAC1 (PubMed:25074804, PubMed:25925950). Increases the presence of activated RAC1 at the leading edge of migrating cells (PubMed:25074804, PubMed:25925950). Required for normal organization of the cytoskeleton, including the actin cytoskeleton, microtubules and the vimentin intermediate filaments (PubMed:27178841). Required for normal cell proliferation, cell migration, and normal formation of lamellipodia (PubMed:27178841). Plays a role in endoplasmic reticulum-associated endosome fission: localizes to endosome membrane tubules and promotes recruitment of TMCC1, leading to recruitment of the endoplasmic reticulum to endosome tubules for fission. Endosome membrane fission of early and late endosomes is essential to separate regions destined for lysosomal degradation from carriers to be recycled to the plasma membrane (By similarity). Required for normal distribution of mitochondria within cells (PubMed:27178841).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202869 | Coro1c (untagged) - Mouse coronin, actin binding protein 1C (Coro1c), (10ug) |
CNY 3,656.00 |
|
| MG207591 | Coro1c (tGFP-tagged) - Mouse coronin, actin binding protein 1C (Coro1c) |
CNY 3,710.00 |
|
| MR207591L3 | Lenti ORF clone of Coro1c (Myc-DDK-tagged) - Mouse coronin, actin binding protein 1C (Coro1c) |
CNY 5,230.00 |
|
| MR207591L4 | Lenti ORF clone of Coro1c (mGFP-tagged) - Mouse coronin, actin binding protein 1C (Coro1c) |
CNY 5,230.00 |
