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Sfmbt1 (BC066188) Mouse Tagged ORF Clone
CAT＃: MR207437
- TrueORF®
Sfmbt1 (Myc-DDK-tagged) - Mouse Scm-like with four mbt domains 1 (cDNA clone MGC:76655 IMAGE:30476111)
ORF Plasmid: tGFP
"BC066188" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Sfmbt, Smr |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207437 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGGGAGCAGCAGCTTGATGCAGATCTTGGGTCTGGTGTAGAAGTAGAAGAATTCAGCTGGGAAG ATTATCTAGAAGAGACAGGCTCCACCACAGTTCCCTACGCATCGTTTAAACATGTGGATATACGTTTGCA AAATGGATTTGCTCCTGGGATGAAGCTGGAGGTAGCTCTGAAAAATGACCCTGAGACTTATTGGGTTGCC ACCATCATCACCGCCTGTGAGCAGTTGCTCCTGCTTCGTTATGAAGGCTATGGGGAAGACCGAAAAGCAG ACTTCTGGTGTGACATCAGGAAAGCTGGCCTCTACCCCATTGGGTGGTGTCAGCAGAACAAGAAGACCCT CGAAGCTCCAGAAGGCATCAGAGATAAAGTGTCTGATTGGAATGCATTCCTACAGCAGACCCTGATAGGA GCCTGTGGTCCTCCCGTGTCTCTGCTAGAGGGCCTCCGTAATGGAAGGAATCCTTTAGATCTCATTGCCC CAGGGTCCAAACTAGAATGCCAGGATTTTCGGGACTCTTTAAGCACTTGGCTTGTTACTGTTGTGGAGAA CATTGGGGGACGGTTGAAGCTGCGGTATGAAGGCCTTGAGAGCCGTGACGGTTTTGAACACTGGTTGTAT TACTTAGATCCATTTCTTCATCACATTGGTTGGGCTGCTCAGCAAGGATGTGACCTTCAGCCCCCATTAG CCATCAAACATCTGAAGAGTGAAGCTGACTGGCAAGAGATTTTGGCCAAAGTGAAAGAGGAAGAGCCATT ACCCTCTTACTTATTTAAGGATAAGCAAGTTATTGGAACCCATGAATTTTCTATAAACATGAAGTTGGAA GCTGTGGACCCGTGGTCTCCTTTTGGGATTTCTCCTGCAACGATTGCTAAGGTGTTTGATGATAAGTACT TTCTAGTGGAAATGGATGACCTTCGGCCAGAAGACCATACAAGGCGATCATTTGTGTGCCATGCTAACAG TCCTGGTATCTTTCCTGTGCAGTGGAGTCTAAAGAATGGTTTACATATCAATCCTCCTCCAGGCTTCCGG AGCCAGGACTTTGACTGGGCCGACTACCTCAAGCAGTGTGGGGCTGAAGCTGCTCCCCAGAAGTGCTTCC CGCAGTCAATTTCTGAGCACCAGTTTAAGGAGAACATGAAACTGGAGGCAGTGAACCCTCTTTTCCCTGA AGAAGTGTGCATTGCCACCGTTACTGCAGTGAGAGGCTCGTACCTGTGGCTCCAGCTAGAAGGTTCTAAG AAGCCTGTACCCGAATTTATTGTAAGTGCAGAGTCGATGAATATATTTCCTTTGGGCTGGTGTGAAACCA ATGGCCATCCTCTCAGTACTCCTCGCCGGGCACGAGGGCATAAACTGAGGAAAATCGCAGTGGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207437 protein sequence
Red=Cloning site Green=Tags(s) MSGEQQLDADLGSGVEVEEFSWEDYLEETGSTTVPYASFKHVDIRLQNGFAPGMKLEVALKNDPETYWVA TIITACEQLLLLRYEGYGEDRKADFWCDIRKAGLYPIGWCQQNKKTLEAPEGIRDKVSDWNAFLQQTLIG ACGPPVSLLEGLRNGRNPLDLIAPGSKLECQDFRDSLSTWLVTVVENIGGRLKLRYEGLESRDGFEHWLY YLDPFLHHIGWAAQQGCDLQPPLAIKHLKSEADWQEILAKVKEEEPLPSYLFKDKQVIGTHEFSINMKLE AVDPWSPFGISPATIAKVFDDKYFLVEMDDLRPEDHTRRSFVCHANSPGIFPVQWSLKNGLHINPPPGFR SQDFDWADYLKQCGAEAAPQKCFPQSISEHQFKENMKLEAVNPLFPEEVCIATVTAVRGSYLWLQLEGSK KPVPEFIVSAESMNIFPLGWCETNGHPLSTPRRARGHKLRKIAVV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC066188 |
| ORF Size | 1395 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC066188, AAH66188 |
| RefSeq Size | 2111 bp |
| RefSeq ORF | 1397 bp |
| Locus ID | 54650 |
| MW | 52.7 kDa |
| Gene Summary | Histone-binding protein, which is part of various corepressor complexes. Mediates the recruitment of corepressor complexes to target genes, followed by chromatin compaction and repression of transcription. Plays a role during myogenesis: required for the maintenance of undifferentiated states of myogenic progenitor cells via interaction with MYOD1. Interaction with MYOD1 leads to the recruitment of associated corepressors and silencing of MYOD1 target genes. Part of the SLC complex in germ cells, where it may play a role during spermatogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217796 | Sfmbt1 (untagged) - Mouse Scm-like with four mbt domains 1 (cDNA clone MGC:76655 IMAGE:30476111), (10ug) |
CNY 6,940.00 |
|
| MG207437 | Sfmbt1 (tGFP-tagged) - Mouse Scm-like with four mbt domains 1 (cDNA clone MGC:76655 IMAGE:30476111) |
CNY 2,950.00 |
|
| MR207437L3 | Lenti ORF clone of Sfmbt1 (Myc-DDK-tagged) - Mouse Scm-like with four mbt domains 1 (cDNA clone MGC:76655 IMAGE:30476111) |
CNY 4,560.00 |
|
| MR207437L4 | Lenti ORF clone of Sfmbt1 (mGFP-tagged) - Mouse Scm-like with four mbt domains 1 (cDNA clone MGC:76655 IMAGE:30476111) |
CNY 4,560.00 |
