Sfmbt1 (BC066188) Mouse Tagged ORF Clone

CAT#: MG207437

  • TrueORF®

Sfmbt1 (tGFP-tagged) - Mouse Scm-like with four mbt domains 1 (cDNA clone MGC:76655 IMAGE:30476111)

ORF Plasmid: DDK tGFP



  "BC066188" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,950.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Sfmbt, Smr
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207437 representing BC066188
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGGGGAGCAGCAGCTTGATGCAGATCTTGGGTCTGGTGTAGAAGTAGAAGAATTCAGCTGGGAAG
ATTATCTAGAAGAGACAGGCTCCACCACAGTTCCCTACGCATCGTTTAAACATGTGGATATACGTTTGCA
AAATGGATTTGCTCCTGGGATGAAGCTGGAGGTAGCTCTGAAAAATGACCCTGAGACTTATTGGGTTGCC
ACCATCATCACCGCCTGTGAGCAGTTGCTCCTGCTTCGTTATGAAGGCTATGGGGAAGACCGAAAAGCAG
ACTTCTGGTGTGACATCAGGAAAGCTGGCCTCTACCCCATTGGGTGGTGTCAGCAGAACAAGAAGACCCT
CGAAGCTCCAGAAGGCATCAGAGATAAAGTGTCTGATTGGAATGCATTCCTACAGCAGACCCTGATAGGA
GCCTGTGGTCCTCCCGTGTCTCTGCTAGAGGGCCTCCGTAATGGAAGGAATCCTTTAGATCTCATTGCCC
CAGGGTCCAAACTAGAATGCCAGGATTTTCGGGACTCTTTAAGCACTTGGCTTGTTACTGTTGTGGAGAA
CATTGGGGGACGGTTGAAGCTGCGGTATGAAGGCCTTGAGAGCCGTGACGGTTTTGAACACTGGTTGTAT
TACTTAGATCCATTTCTTCATCACATTGGTTGGGCTGCTCAGCAAGGATGTGACCTTCAGCCCCCATTAG
CCATCAAACATCTGAAGAGTGAAGCTGACTGGCAAGAGATTTTGGCCAAAGTGAAAGAGGAAGAGCCATT
ACCCTCTTACTTATTTAAGGATAAGCAAGTTATTGGAACCCATGAATTTTCTATAAACATGAAGTTGGAA
GCTGTGGACCCGTGGTCTCCTTTTGGGATTTCTCCTGCAACGATTGCTAAGGTGTTTGATGATAAGTACT
TTCTAGTGGAAATGGATGACCTTCGGCCAGAAGACCATACAAGGCGATCATTTGTGTGCCATGCTAACAG
TCCTGGTATCTTTCCTGTGCAGTGGAGTCTAAAGAATGGTTTACATATCAATCCTCCTCCAGGCTTCCGG
AGCCAGGACTTTGACTGGGCCGACTACCTCAAGCAGTGTGGGGCTGAAGCTGCTCCCCAGAAGTGCTTCC
CGCAGTCAATTTCTGAGCACCAGTTTAAGGAGAACATGAAACTGGAGGCAGTGAACCCTCTTTTCCCTGA
AGAAGTGTGCATTGCCACCGTTACTGCAGTGAGAGGCTCGTACCTGTGGCTCCAGCTAGAAGGTTCTAAG
AAGCCTGTACCCGAATTTATTGTAAGTGCAGAGTCGATGAATATATTTCCTTTGGGCTGGTGTGAAACCA
ATGGCCATCCTCTCAGTACTCCTCGCCGGGCACGAGGGCATAAACTGAGGAAAATCGCAGTGGTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207437 representing BC066188
Red=Cloning site Green=Tags(s)

MSGEQQLDADLGSGVEVEEFSWEDYLEETGSTTVPYASFKHVDIRLQNGFAPGMKLEVALKNDPETYWVA
TIITACEQLLLLRYEGYGEDRKADFWCDIRKAGLYPIGWCQQNKKTLEAPEGIRDKVSDWNAFLQQTLIG
ACGPPVSLLEGLRNGRNPLDLIAPGSKLECQDFRDSLSTWLVTVVENIGGRLKLRYEGLESRDGFEHWLY
YLDPFLHHIGWAAQQGCDLQPPLAIKHLKSEADWQEILAKVKEEEPLPSYLFKDKQVIGTHEFSINMKLE
AVDPWSPFGISPATIAKVFDDKYFLVEMDDLRPEDHTRRSFVCHANSPGIFPVQWSLKNGLHINPPPGFR
SQDFDWADYLKQCGAEAAPQKCFPQSISEHQFKENMKLEAVNPLFPEEVCIATVTAVRGSYLWLQLEGSK
KPVPEFIVSAESMNIFPLGWCETNGHPLSTPRRARGHKLRKIAVV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC066188
ORF Size 1397 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC066188, AAH66188
RefSeq Size 2111 bp
RefSeq ORF 1397 bp
Locus ID 54650
Gene Summary Histone-binding protein, which is part of various corepressor complexes. Mediates the recruitment of corepressor complexes to target genes, followed by chromatin compaction and repression of transcription. Plays a role during myogenesis: required for the maintenance of undifferentiated states of myogenic progenitor cells via interaction with MYOD1. Interaction with MYOD1 leads to the recruitment of associated corepressors and silencing of MYOD1 target genes. Part of the SLC complex in germ cells, where it may play a role during spermatogenesis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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